Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 12 (december 2020) : 1532-1537

In-vivo Efficacy of Poultry and Fish Probiotics on Green Mussel, Perna viridis, Resistance against Vibrio alginolyticus

A.A. Laith, S. Badr, M.K. Ros-Amira, I.S. Hassan, A.W.M. Effendy, M. Najiah
1School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
Cite article:- Laith A.A., Badr S., Ros-Amira M.K., Hassan I.S., Effendy A.W.M., Najiah M. (2020). In-vivo Efficacy of Poultry and Fish Probiotics on Green Mussel, Perna viridis, Resistance against Vibrio alginolyticus. Indian Journal of Animal Research. 54(12): 1532-1537. doi: 10.18805/ijar.B-1158.

ABSTRACT

The efficiency of using lactic acid bacteria derived from chicken to be used in fish to limit microbial infections in aquaculture was investigated. Gram-positive isolates were cultured on Man Rogosa Sharp agar (MRS). Identification of bacterial strains was done by analyzing nucleotide sequence of the 16S rRNA gene. Lactobacillus plantarum (98%) was isolated from Oreochromis niloticus and Enterococcus durans (96%) was isolated from Gallus gallus. The sequences were deposited in GenBank with the Accession No. of MF197402 and MF197403, respectively. Experimental mussels were divided into 3 groups; control group fed commercial feed only, Treatment 1 and Treatment 2 were fed with commercial feed supplemented with the candidate probiotics isolated from chicken and fish, respectively, for 21 days. P. viridis were immersed in V. alginolyticus 1.0 ×106 CFU/ml. L. plantarum significantly affected haemocyte count, lysozyme activity and mortality rate, whereas E. durans significantly affect lysozyme activity only. 

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