Indian Journal Of Animal Research

In-vivo Efficacy of Poultry and Fish Probiotics on Green Mussel, Perna viridis, Resistance against Vibrio alginolyticus

DOI: 10.18805/ijar.B-1158    | Article Id: B-1158 | Page : 1532-1537
Citation :- In-vivo Efficacy of Poultry and Fish Probiotics on Green Mussel, Perna viridis, Resistance against Vibrio alginolyticus.Indian Journal Of Animal Research.2020.(54):1532-1537
A.A. Laith, S. Badr, M.K. Ros-Amira, I.S. Hassan, A.W.M. Effendy, M. Najiah laith.abdul@umt.edu.my
Address : School of Fisheries and Aquaculture Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
Submitted Date : 7-06-2019
Accepted Date : 9-04-2020
First Online:

Abstract

The efficiency of using lactic acid bacteria derived from chicken to be used in fish to limit microbial infections in aquaculture was investigated. Gram-positive isolates were cultured on Man Rogosa Sharp agar (MRS). Identification of bacterial strains was done by analyzing nucleotide sequence of the 16S rRNA gene. Lactobacillus plantarum (98%) was isolated from Oreochromis niloticus and Enterococcus durans (96%) was isolated from Gallus gallus. The sequences were deposited in GenBank with the Accession No. of MF197402 and MF197403, respectively. Experimental mussels were divided into 3 groups; control group fed commercial feed only, Treatment 1 and Treatment 2 were fed with commercial feed supplemented with the candidate probiotics isolated from chicken and fish, respectively, for 21 days. P. viridis were immersed in V. alginolyticus 1.0 ×106 CFU/ml. L. plantarum significantly affected haemocyte count, lysozyme activity and mortality rate, whereas E. durans significantly affect lysozyme activity only. 

Keywords

Enterococcus durans Haemocyte Lactobacillus plantarum lysozyme Perna viridis

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