Effects of Glycosaminoglycan from Urechis Unicinctus on the P2Y12 Receptor Signaling Pathway in Rat Platelets

DOI: 10.18805/ijar.B-1149    | Article Id: B-1149 | Page : 24-30
Citation :- Effects of Glycosaminoglycan from Urechis Unicinctus on the P2Y12 Receptor Signaling Pathway in Rat Platelets.Indian Journal Of Animal Research.2020.(54):24-30
Chunying Yuan, Fei Miao, Jinghong Li and Qingman Cui ycy201388@163.com
Address : Tianjin Marine Environmental Protection and Restoration Technology Engineering Center; Tianjin Key Laboratory of Marine Resource and Chemistry; College of Marine & Environment, Tianjin University of Science and Technology, Tianjin, China.
Submitted Date : 15-05-2019
Accepted Date : 3-10-2019

Abstract

This study aims to investigate the effects of glycosaminoglycan (GAG) from Urechis unicinctus on the P2Y12 receptor signaling pathway in rat platelets. The concentrations of cyclic adenosine monophosphate (cAMP), thromboxane B2 (TXB2) and glycoprotein IIb/IIIa (GPIIb/IIIa) in rat platelets were determined using enzyme-linked immunosorbent assay (ELISA) kits. The phosphorylation levels of protein kinase A (PKA) and vasodilator-stimulated phosphoprotein (VASP) in rat platelets were detected through Western blot. The expression of P2Y12 gene in rat platelets was analyzed via real-time fluorescence quantitative PCR and reverse-transcription PCR. It was observed that GAG significantly increased the cAMP content (p < 0.05, p < 0.01) and decreased the TXB2 and GPIIb/IIIa concentrations (p < 0.05, p < 0.01) in rat platelets. GAG significantly enhanced the phosphorylation levels of PKA and VASP in rat platelets (p < 0.01) and had a synergistic effect with the P2Y12 receptor blocker. GAG significantly reduced the expression level of P2Y12 gene in rat platelets (p < 0.01). We speculated that GAG from U. unicinctus inhibited platelet aggregation in rats through the P2Y12 receptor signaling pathway.

Keywords

ADP receptor Glycosaminoglycan P2Y12 signaling pathway Urechis unicinctus

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