Microsatellite DNA Markers and Their Correlation with Body Traits in Landrace, Duroc and Henan Western Black Pigs 

DOI: 10.18805/ijar.B-1148    | Article Id: B-1148 | Page : 255-260
Citation :- Microsatellite DNA Markers and Their Correlation with Body Traits in Landrace, Duroc and Henan Western Black Pigs.Indian Journal Of Animal Research.2021.(55):255-260
You.Bing. Yang , Jun.Ping. Bian, You.Zhi. Pang and Ying. Lei bianjunping527@163.com
Address : College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471023 China.
Submitted Date : 15-05-2019
Accepted Date : 26-09-2019

Abstract

Twelve microsatellite loci were selected from the Food and Agriculture Organization of the United Nations to examine the genetic structure and relationship between microsatellite DNA and body traits including height, back height, body length, chest width, chest depth, head Length, Ear area, and forehead width. Twelve microsatellite loci analyzed were polymorphic in Landrace, Duroc and Henan Western Black Pigs. The total number of alleles found for the twelve microsatellite loci amounted to 150.The average expected heterozygositie ranged from 0.1287 to 0.8301 among twelve loci, and the average polymorphic information content ranged from 0.1168 to 0.7894. The results of cluster analysis showed that the genetic distance between Henan Western Black Pigs and Duroc was least 0.1414. Microsatellite loci of SW72 were significantly associated with back height and chest depth (P<0.05), while SO002 were significantly associated with chest depth and head Length (P<0.05). Finally, the results showed that Henan Western Black Pigs has a high level of genetic polymorphism and genetic differentiation. Cluster analysis shows that the relationship between Black Pigs and Duroc is closer. These were significantly associated with body traits in five microsatellite loci, including SW72, SO002, SW632, SO090 and SW1828 (P<0.05). These results provided scientific reference for the further cultivation and utilization of black pigs. 

Keywords

Genetic Diversity Henan Western Black Pigs Heterozygositie Microsatellite Polymorphism

References

  1. Bai, J.Y., Yang, Y.B., Wang, Y.Q., Zhang, X.H. and Pang, Y.Z. (2015). Polymorphism analysis of three Chinese indigenous sheep breeds by microsatellite markers. Indian Journal of Animal Research. 49: 585-590.
  2. Bai, J.Y., Pang, Y.Z., Zhang, X.H., Yun, Y.X. and Qi, Y.X. (2016a). Microsatellite analysis of genetic diversity in quail populations from China. Brazilian Journal of Poultry Science. 18: 519-524.
  3. Bai, J.Y., Pang, Y.Z., Wu, S.J., Yu, M.Q. and Zhang, X.H. (2016b). Analysis of genetic diversity of four quails by microsatellite markers. Indian Journal of Animal Research. 50: 1-7.
  4. Behl, R., Behl, J., Tantia, M.S., Nahardeka, N., Das, G.C. Kumar, K.S. and Vijh, R.K.(2017). Evaluation of 24 microsatellite markers for parentage exclusion in three indigenous pig types of India. Indian Journal of Animal Science. 87: 443-446.
  5. Chang, W.H., Chu, H.P., Jiang, Y.N., Li, S.H., Wang, Y., Chen, C.H., Chen, K.J., Lin, C.Y. and Ju, Y.T. (2009). Genetic variation and phylogenetics of Lanyu and exotic pig breeds in Taiwan analyzed by nineteen microsatellite markers. Journal of Animal Science. 87: 1-8.
  6. Cortés, O., Martinez, A.M., Cañon, J., Sevane, N., Gama L.T., Ginja, C., Landi, V., et al. (2016). Conservation priorities of Iberoamerican pig breeds and their ancestors based on microsatellite information. Heredity. 117: 14-24.
  7. Deshmukh, B., Kumar, D., Kashyap, N. and Sharma, D. (2015). Study of genetic polymorphism of various chicken breeds using microsatellite markers. Indian Journal of Animal Research. 49: 1-7.
  8. Gélvez, I.M., Pérez, E.P. and Martinez, T.I.C. (2015). Genetic variation in domestic pig Sus scrofa domestica populations in Cordoba-    Colombia based on microsatellite markers. Revista Mexicana de Ciencias Pecuarias. 6: 443-452. 
  9. Guo, X. and Elston, R.C. (1999). Linkage information content of polymorphic genetic markers. Human Heredity. 49: 112-8.
  10. Jiménez-Mena, B., Hospital, F. and Bataillon, T. (2016). Heterogeneity in effective population size and its implications in conservation genetics and animal breeding. Conservation Genetics Resources. 8: 1-7.
  11. Kavitha, S.T., Subramanian, A., Sivaselvam, S.N., Thiagarajan, R. and Balasubramanian, S. (2015). Genetic characterisation of Tiruchy Black sheep of Tamil Nadu using microsatellite markers. Indian Journal of Animal Research. 49: 320-324.
  12. Kierepka, E.M., Unger, S.D., Keiter, D.A., Beasley, J.C., Rhodes, O.E., Cunningham, F.L. and Piaggio, A.J. (2016). Identification of robust microsatellite markers for wild pig fecal DNA. Journal of Wildlife Management. 80: 1120-1128.
  13. Kim, K.S., Yeo, J.S. and Kim, J.W. (2002). Assessment of genetic diversity of Korean native pig (Sus scrofa) using AFLP markers. Genes & Genetic Systems. 77: 361-368. 
  14. Li, Y.P, He, Q.M., Cai, S.H. and Chen, J.Q. (1999). Preliminary evaluation of xenotransplantation and utilization of native pig breeds in China. Chinese Journal of Reparative and Reconstructive Surgery. 13: 43-46.
  15. Michailidou, S., Kalivas, A., Ganopoulos, I., Stea, E., Michailidis, G., Tsaftaris, A. and Argiriou, A. (2014). A multi-farm assessment of Greek black pig genetic diversity using microsatellite molecular markers. Genetics and Molecular Research. 13: 2752-2765.
  16. Montenegro, M., Llambí, S., Castro, G., Barlocco, N., Vadell, A., Landi, V., Delgado, J.V. and Martínez, A. (2015). Genetic characterization of Uruguayan Pampa Rocha pigs with microsatellite markers. Genetics and Molecular Biology. 38: 48-54.
  17. Nagy, S., Poczai, P., Cernák, I., Gorji, A.M., Hegedûs, G. and Taller, J. (2012). PIC calc: an online program to calculate polymorphic information content for molecular genetic studies. Biochemical Genetics. 50: 670-672. 
  18. Oh, J.D., Song, K.D., Seo, J.H., Kim, D.K., Kim, S.H, Seo, K.S., Lim, H.T. and Lee, J.B. et al. (2014).Genetic traceability of black pig meats using microsatellite markers. Asian Australasian Journal of Animal Sciences. 27: 926-931.
  19. Phythian, C.J., Cripps, P.J., Michalopoulou, E., Jones, P.H., Grove-White, D. and Clarkson, M.J. et al. (2012). Reliability of indicators .of sheep welfare assessed by a group observation method. Veterinary Journal. 193: 257-263.
  20. Rátky, J., Egerszegi, I., Toth, P., Keonuchan, S., Nagai, T., Kikuchi, K., Manábe, N. and Brüssow, K.P. (2013).Saving genetic resources of native pigs in Occidental and Oriental countries - practical examples of the characterization and utilization of native pigs in Hungary and Laos. Journal of Reproduction and Development. 59: 437-441.
  21. Revidatti, M.A., Delgado Bermejo, J.V., Gama, L.T., Landi Periati, V. and Ginja, C. et al. (2014). Genetic characterization of local criollo pig breeds from the americas using microsatellite markers. Journal of Animal Science. 92: 4823-4832.
  22. Sahoo, N.R., Naska,r S., Banik, S. and Pankaj, P.K. (2015a). Microsatellite based diversity analysis of native pigs of North-Eastern India. Indian Journal of Animal Research. 50: 831-839.
  23. Sahoo, N.R., Nesa, N., Naskar, S., Banik, S., Pankaj, P.K. and Sahoo, M. (2016). Microsatellite and mitochondrial diversity analysis of native pigs of Indo-Burma biodiversity hotspot. Animal Biotechnology. 27: 52-59.
  24. Sahoo, N.R., Nesa, N., Naskar, S., Banik, S. and Pankaj, P.K. (2015b). Genetic diversity analysis of Ghoongroo pig based on microsatellite markers. Indian Journal of Animal Sciences. 85: 1215-1219. 
  25. Session, T.R. (2011). Draft guidelines on molecular genetic characterization of animal genetic resources. Rome: Commission on genetic for food and agriculture sixth session. 61-63.
  26. Vlot, J., Staals, L.M., Wijnen, R.M., Stolker, R.J. and Bax, K.N. (2015). Optimizing working space in laparoscopy: CT measurement of the influence of small body size in a porcine model. Journal of Pediatric Surgery. 50: 465-471.
  27. Wang, X., Cao, H.H., Geng, S.M. and Li, H.B. (2004). Genetic diversity of 10 indigenous pig breeds in China by using microsatellite markers. Asian-Australasian Journal of Animal Sciences. 17: 1219-1222.
  28. Yu, G.C., Tang, Q.Z., Long, K.R., Che, T.D., Li, M.Z. and Shuai S.R. (2015). Effectiveness of microsatellite and single nucleotide polymorphism markers for parentage analysis in European domestic pigs. Genetics and Molecular Research. 14: 1362-1370.
  29. Zhang, C.Y. and Plastow, G. (2011). Genomic diversity in pig (Sus scrofa) and its comparison with human and other livestock. Current Genomics. 12: 138-146. 

Global Footprints