Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 8 (august 2020) : 957-961

Molecular Characterization of the TLR2 Gene in Datong Yak

Shuai Peng, Lang Chen, Tian-Yu Zheng, Li Zhang, Zhuo Li, Xiao-Jing Tian, Zhong-Ren Ma, Li-Xia Liu
1College of Life Science and Engineering, Northwest Minzu University, Lanzhou-730 030, China.
Cite article:- Peng Shuai, Chen Lang, Zheng Tian-Yu, Zhang Li, Li Zhuo, Tian Xiao-Jing, Ma Zhong-Ren, Liu Li-Xia (2019). Molecular Characterization of the TLR2 Gene in Datong Yak. Indian Journal of Animal Research. 54(8): 957-961. doi: 10.18805/ijar.B-1138.

ABSTRACT

The coding region of Datong yak’s TLR2 gene was amplified and subjected to sequence characterization. The coding region of the Datong yak TLR2 gene comprised a single ORF of 2355 nucleotides that coded for 784 amino acids with translatable products. The coding region of the TLR2 gene of the Datong yak contained two nucleotide mutation sites, namely, G677A and G1587A. G677A exhibited a missense mutation. After comparing nucleotide and amino acid sequences among related species and constructing the phylogenetic relationships, Datong yak sequences were shown to be highly similar to those of Bos taurus. The Datong yak TLR2 protein simultaneously possessed leucine-rich repeats, a TIR domain and an aldehyde dehydrogenase active site. Results showed that the protein plays an important role in the body’s immune regulation mechanism.

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