Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 55 issue 3 (march 2021) : 271-276

Novel alternative splice variant of IGF-1R and its mRNA expression patterns in BaMa and Landrace pigs

Rui Yang, Lijie Dong, Songcai Liu, Yunyun Cheng, Wenzhen Wei, Jie Song, Haoyang Li, Hongwei Geng, Linlin Hao, Ying Zhang
1College of Animal Science, Jilin University, Changchun, Jilin 130 062, China.
Cite article:- Yang Rui, Dong Lijie, Liu Songcai, Cheng Yunyun, Wei Wenzhen, Song Jie, Li Haoyang, Geng Hongwei, Hao Linlin, Zhang Ying (2019). Novel alternative splice variant of IGF-1R and its mRNA expression patterns in BaMa and Landrace pigs. Indian Journal of Animal Research. 55(3): 271-276. doi: 10.18805/ijar.B-1107.
The transcript variants of Insulin-like growth factor 1 receptor (IGF-1R) and their expression profiles had never been illuminated in pigs until now. Herein, we identified IGF-1R AS02 as a novel splice variant of IGF-1R gene by RT-PCR and analyzed its mRNA expression level by qRT-PCR in liver, cartilage and muscle tissues, while also detecting the single-nucleotide polymorphism (SNP) site near the splice site of the IGF-1R gene (in intron 19) of BaMa and Landrace pigs. Results demonstrated that the IGF-1R AS02 variant showed a significantly (P<0.05) higher expression level in cartilage than in muscle and liver across two pig breeds respectively. The expression level of the normal transcript (IGF-1R ISO01) of IGF-1R in cartilage was markedly lower than that in the other two tissues (P<0.05). In cartilage, IGF-1R ISO01 expression was higher in BaMa than in Landrace (P<0.05), while the expression level of IGF-1R AS02 was lower in BaMa than in Landrace (P<0.05). The SNP was detected in intron 19 of the IGF-1R gene of BaMa and Landrace pigs. These results contributed to facilitating a better understanding of IGF-1R gene in pigs.
  1. Angeloni, D., Duh, F.M., Moody, M., Dean, M., Zabarovsky, E.R., Sentchenko. V. (2003). C to A single nucleotide polymorphism in intron 18 of the human MST1R (RON) gene that maps at 3p21.3. Mol Cell Probes. 17: 55-57.
  2. Aneet Kour, A.K., Chakravarty, T., Karuthadurai, E. R. and Varinder, R. (2017). Genetic variability in exon 5 region of GH1 gene and its effect on milk production and milk composition traits in Karan Fries cattle. Indian Journal Of Animal Research. 53: 14-18.
  3. Ast, G. (2004). How did alternative splicing evolve? Nat Rev Genet. 5: 773.
  4. Baker, J.,p Liu, J. P., Robertson, E. J. and Efstratiadis, A. (1993). Role of insulin-like growth factors in embryonic and postnatal growth. Cell. 75: 73-82.
  5. Brogna, S., Wen, J. (2009). Nonsense-mediated mRNA decay (NMD) mechanisms. Nat Struct Mol Biol. 16:107-113.
  6. Charge, S.B. and Rudnicki, M.A. (2004). Cellular and molecular regulation of muscle regeneration. Physiol Rev. 84: 209-238.
  7. Cheng, Y., Liu, S., Zhang, X., Wu, Q., Li, S., Fu, H. (2016). Expression Profiles of IGF-1R Gene and Polymorphisms of its Regulatory Regions in Different Pig Breeds. Protein J. 35: 231-236.
  8. Delafontaine, P., Song, Y.H., Li, Y. (2004). Expression, regulation, and function of IGF1, IGF1R, and IGF1 binding proteins in blood vessels. Arterioscler Thromb Vasc Biol. 4: 435.
  9. Guntur, A.R., Rosen, C.J. (2013). IGF-1 regulation of key signaling pathways in bone. Bonekey Rep. 2: 437.
  10. Liu, H.B., Lv, P.R., He, R.G., Yang, X.G., Qin, X.E., Pan, T.B. (2010). Cloned Guangxi Bama mini pig (Sus scrofa) and its offspring have normal reproductive performance. Cell Reprogram. 12: 543-550.
  11. Liu, J.P., Baker, J., Perkins, A.S., Robertson, E.J. and Efstratiadis, A. (1993). Mice carrying null mutations of the genes encoding insulin-like growth factor I (IGF-1) and type1 IGF receptor (IGF-1R). Cell. 75: 59-72.
  12. Meenakshi, J., Surender, K., Pushpa, K., Parveen, B. and Vijay, K. C. (2017). Determination of Cry1Ac copy number in transgenic pigeonpea plants using quantitative real time PCR. Legume Research An International Journal. 4: 643-648.
  13. Li, X., Suh, Y., Kim, E., Moeller, S.J., Lee, K. (2012). Alternative splicing and developmental and hormonal regulation of porcine comparative gene identification-58 (CGI-58) mRNA. J Anim Sci. 90: 4346-4354.
  14. LeRoith, D., Werner, H., Beitner-Johnson, D. and Roberts, C.T. Jr. (1995). Molecular and cellular aspects of the insulin-like growth factor I receptor. Endocrine Reviews. 16: 143-163.
  15. Ma, J., Yang, J., Zhou, L., Ren, J., Liu, X., Zhang, H. (2014). A splice mutation in the PHKG1 gene causes high glycogen content, low meat quality in pig skeletal muscle. PLoS Genet. 10: e1004710.
  16. Singh, R., Rai, T.S., Sharma, N.S., Arora, A.K. and Paviter, K. (2017). Evaluation of a Real time polymerase chain reaction assay for the detection of aflatoxin/sterigmatocystin producing strains of Aspergillus spp. Indian Journal Of Animal Research. 51: 676-678.
  17. Sammeth, M., Foissac, S., Guigo, R. (2008). A general definition and nomenclature for alternative splicing events. PLoS Comput Biol, 4: e1000147.
  18. Seo, S., Takayama, K., Uno, K., Ohi, K., Hashimoto, R., Nishizawa, D. (2013). Functional analysis of deep intronic SNP rs13438494 in intron 24 of PCLO gene. PLoS One. 8: e76960.
  19. Swindle, M.M., Makin, A., Herron, A.J., Clubb, F.J. Jr., Frazier, K.S. (2012). Swine as models in biomedical research and toxicology testing. Vet Pathol. 49: 344-356.
  20. Wu, F.C., Wei, H., Gan, S.X., Wang, A.D. (2001). Analysis of genetic diversity in Bama and Guizhou miniature pigs by RAPD. ShiYanShengWuXueBao. 34: 115-119.
  21. Wang, E.T., Sandberg, R., Luo, S., Khrebtukova, I., Zhang, L., Mayr, C. (2008). Alternative isoform regulation in human tissue transcriptomes. Nature. 456: 470-476.
  22. Yang, F., Du, X., Wang, Y., Wang, C., Huang, C., Xiao, Q. (2015). Characterization and functional analysis of porcine estrogen-related receptors and their alternative splicing variants. J Anim Sci. 93: 4258-4266.
  23. Zhang, X., Zhou, Y., Pan, C., Lei, C., Dang, R., Chen, H., Lan, X. (2015). Novel alternative splice variants of NFIX and their diverse mRNA expression patterns in dairy goat. Gene. 569: 250-258.
  24. Zhou, Y., Sun, J., Li, C., Wang, Y., Li, L., Cai, H. (2014). Characterization of transcriptional complexity during adipose tissue development in bovines of different ages and sexes. PLoS One. 9: e101261. 

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