Expression patterns and immunohistochemical analysis of ERK, HRAS and MEK1 proteins during ovarian prehierarchical follicular development in Zi geese (Anser cygnoides)

DOI: 10.18805/ijar.B-1069    | Article Id: B-1069 | Page : 790-795
Citation :- Expression patterns and immunohistochemical analysis of ERK, HRAS and MEK1 proteins during ovarian prehierarchical follicular development in Zi geese (Anser cygnoides).Indian Journal of Animal Research.2022.(56):790-795
H. Lu, C.T. Sello, C. Liu, Y. Sui, C. Xu, Y. Sun, J. Liu, Y. Zhou, S. Li, W. Yang, P. Msuthwana, J. Hu, Y. Sun sunyongfeng1977@126.com
Address : Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Xincheng Street, No: 2888. Changchun City, Jilin Agricultural University, Changchun, People’s Republic of China.
Submitted Date : 21-11-2018
Accepted Date : 7-02-2019


The objective of this study was to investigate the spatiotemporal expression levels and protein localization of extracellular regulated MAP kinase (ERK), HRas proto-oncogene, GTPase (HRAS), and mitogen-activated protein kinase kinase 1 (MEK1) genes in ovarian prehierarchical follicles of geese. The prehierarchical follicles from healthy laying geese (n=6) at the age of 35 to 37 weeks were harvested. The relative expression levels of ERK, HRAS, and MEK1 in various sized prehierarchical follicles were detected by real-time quantitative polymerase chain reaction (RT-qPCR), western blotting, and follicular wall localization was investigated by using immunohistochemistry. The results revealed that the candidate genes were expressed differently at mRNA and protein levels at five stages of prehierarchical follicle development. These results suggest that ERK, HRAS, and MEK1 might be associated to the key biological mechanisms regulating Zi geese folliculogenesis.


Folliculogenesis Immunohistochemistry MAPK RT-qPCR Western blotting.


  1. Bi, D.Y., Liu, Q., Zhan, Y., Li, D., Zou, Y.F., Ma, M.Z., Chang, X.R. (2017). Effect of herbal cake- partitioned moxibustion on MEK1/    2 and ERK1/2 expressions of gastric tissues in rats with spleen deficiency syndrome. J. Acupunct. Tuina Sci., 15: 305-310.
  2. Chen, J., Cao, J., Luo, Y., Xie, L., Song, J., Xue, W., Song, J. (2014). Expression of ERK and p-ERK proteins of ERK signaling pathway in the kidneys of fluoride-exposed carp (Cyprinus carpio). Acta Histochem., 116: 1337-1341. 
  3. De Luca, A., Maiello, M.R., D’Alessio, A., Pergameno, M., Normanno, N. (2012). The RAS/RAF/MEK/ERK and the PI3K/AKT signalling pathways: role in cancer pathogenesis and implications for therapeutic approaches. Expert Opin Ther Targets., 16: 17-27. 
  4. Fan, H.Y., Liu, Z., Shimada, M., Sterneck, E., Johnson, P.F., Hedrick, S.M., Richards, J.S. (2009). MAPK3/1 (ERK1/2) in ovarian granulosa cells are essential for female fertility. Science, 324: 938-941. 
  5. Gong, R., Sun, D., Zhong, X., Sun, Y., Li, L. (2015). MEK1 expression and its relationship with clinical pathological features in hepatocellular carcinoma. Int. J. Clin. Exp. Med., 8: 4087-4093. 
  6. Gu, J., Han, T., Ma, R.H., Zhu, Y.L., Jia, Y.N., Du, J.J., Guo, X. (2014). SHP2 promotes laryngeal cancer growth through the Ras/Raf/    Mek/Erk pathway and serves as a prognostic indicator for laryngeal cancer. Int. J. Oncol., 44: 481-490. 
  7. Hsu, Y.F., Lee, T.S., Lin, S.Y., Hsu, S.P., Juan, S.H., Hsu, Y.H., Lee, W.S. (2007). Involvement of Ras/Raf-1/ERK actions in the magnolol-induced upregulation of p21 and cell-cycle arrest in colon cancer cells. Mol. Carcinogen., 46: 275-283. 
  8. Hussein, M.R. (2005). Apoptosis in the ovary: molecular mechanisms. Hum. Reprod. Update., 11: 162-177. 
  9. Johnson, A.L. (2003). Intracellular mechanisms regulating cell survival in ovarian follicles. Anim. Reprod Sci., 78: 185-201.
  10. Johnson, A.L. and Woods, D.C. (2009). Dynamics of avian ovarian follicle development: cellular mechanisms of granulosa cell differentiation. Gen. Com. Endocrinol., 163: 12-17. 
  11. Lee, S.H., Oh, H.J., Kim, M.J., Kim, G.A., Choi, Y.B., Jo, Y.K., Lee, B.C. (2017). Oocyte maturation-related gene expression in the canine oviduct, cumulus cells, and oocytes and effect of co-culture with oviduct cells on in vitro maturation of oocytes. J. Assist. Reprod. Genet., 34: 929-938. 
  12. Liang, B., Wang, S., Zhu, X.G., Yu, Y.X., Cui, Z.R.,Yu, Y.Z. (2005). Increased expression of mitogen-activated protein kinase and its upstream regulating signal in human gastric cancer. World J. Gastroenterol., 11: 623-628. 
  13. Lin, Z., Zhang, C., Zhang, M., Xu, D., Fang, Y., Zhou, Z., Zhang, X. (2014). Targeting cadherin-17 inactivates Ras/Raf/MEK/ERK signaling and inhibits cell proliferation in gastric cancer. PloS one, 9: e85296. 
  14. Liu, Y., Beyer, A., Aebersold, R. (2016). On the dependency of cellular protein levels on mRNA abundance. Cell, 165: 535-550.
  15. Lovell, T.M., Gladwell, R.T., Groome, N.P., Knight, P.G. (2003) Ovarian follicle development in the laying hen is accompanied by divergent changes in inhibin A, inhibin B, activin A and follistatin production in granulosa and theca layers. J. Endocrinol., 177: 45-55.
  16. Newlaczyl, A.U., Coulson, J.M., Prior, I.A. (2017). Quantification of spatiotemporal patterns of Ras isoform expression during development. Sci. Rep., 7: 41297. 
  17. Omori, S., Hida, M., Ishikura, K., Kuramochi, S., Awazu, M. (2000). Expression of mitogen-activated protein kinase family in rat renal development. Kidney Int., 58: 27-37. 
  18. Park, J.C., Jeong, W.J., Kim, M.Y., Min, D., Choi, K.Y. (2016). Retinoic acid-mediated hras stabilization induces neuronal differentiation of neural stem cells during brain development. J. Cell Sci., 129: 2995-3007.
  19. Tepekoy, F. and Akkoyunlu, G. (2016). The effect of FSH and activin A on Akt and MAPK1/3 phosphorylation in cultured bovine ovarian cortical strips. J. Ovarian Res., 9: 13. 
  20. Vairaktaris, E., Papakosta, V., Derka, S., Vassiliou, S., Nkenke, E., Spyridonidou, S., Moulavassili, P. (2008). H-ras and c-fos exhibit similar expression patterns during most stages of oral oncogenesis. Vivo, 22: 621-628. 
  21. Zhang, X., Kang, B., Zhang, L.N., Guo, J.R., Jiang, D.M., Ji, H., Zhen, L., Yang, H.M. (2013). Gene expression profiles of LH, prolactin and their receptors in female Zi geese (Anser cygnoides) during development. Folia Biol., 61: 59-64. 

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