Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 8 (august 2020) : 1034-1038

Molecular Detection of Neospora caninum from Naturally Infected Dogs in Van Province, East Turkey

Ali Bilgin Yilmaz, Yaşar Göz, Özlem Orunç Kilinç, Vural Denizhan
1Faculty of Health Sciences, Van Yuzuncu Yil University, 65100, Turkey.
Cite article:- Yilmaz Bilgin Ali, Göz Yaşar, Kilinç Orunç Özlem, Denizhan Vural (2020). Molecular Detection of Neospora caninum from Naturally Infected Dogs in Van Province, East Turkey. Indian Journal of Animal Research. 54(8): 1034-1038. doi: 10.18805/ijar.B-1051.

ABSTRACT

Neospora caninum is a coccidian parasite causing abortion in cattle and neurological problems in horses. Dogs are definitive hosts of N. caninum. Polymerase chain reaction is the most specific method used for the detection of N. caninum oocytes. In the present study, a total of 100 fecal samples were collected from naturally infected dogs. Of the 100 samples analyzed, 11 of them were detected with Hammondia/Neospora-like oocytes. Genomic DNA was isolated using a commercially available DNA extraction kit. The Nc5 gene specific to N. caninum was amplified by PCR and two of the eleven samples with Hammondia/Neospora-like oocytes formed ~337 bp repeatable band. In conclusion, N. caninum, which has been shown to cause neurological disorders in dogs and to abortion in cattle, was detected in naturally infected dogs in Van Province in Polymerase chain reaction. 

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