Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 5 (may 2020) : 549-552

Comparative evaluation of the in-vitro viability of canine and human blood preserved in citrate phosphate dextrose adenine (CPDA)-1 anticoagulated blood bag

R.I. Udegbunam, C.S. Njaka, H.N. Okereke, S.O. Udegbunam
1Department of Veterinary Surgery, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.
Cite article:- Udegbunam R.I., Njaka C.S., Okereke H.N., Udegbunam S.O. (2019). Comparative evaluation of the in-vitro viability of canine and human blood preserved in citrate phosphate dextrose adenine (CPDA)-1 anticoagulated blood bag. Indian Journal of Animal Research. 54(5): 549-552. doi: 10.18805/ijar.B-1039.

ABSTRACT

Two hundred and fifty millilitres of blood each were drawn from healthy dogs (n=3) and volunteer human donors (n=3) into citrate phosphate dextrose adenine -1 anti-coagulated blood bags and preserved for 21 days. On days 0, 3, 7, 14 and 21, selected hematological and biochemical parameters were evaluated. Red blood cells counts of canine and human blood showed no significant (p>0.05) difference till days 14 and 21 respectively. Mean corpuscular value (MCV) of canine blood on day 21 was significantly (p<0.05) higher than that of human blood. Erythrocyte catalase (CAT) and glutathione (GSH) progressively decreased while plasma potassium ion concentration of canine and human blood progressively increased. On day 21, percentage decrease in canine RBC antioxidants was significantly higher when compared with that of human blood. The progressive decrease in RBC’s CAT and GSH suggests increased oxidative stress while progressive increase in K+ concentration and MCV suggests RBC membrane damage. 

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