Gender-Specific differences in pulsed magnetic field exposed to diabetic neuropathic rats

DOI: 10.18805/ijar.B-1026    | Article Id: B-1026 | Page : 723-728
Citation :- Gender-Specific differences in pulsed magnetic field exposed to diabetic neuropathic rats.Indian Journal Of Animal Research.2020.(54):723-728
Iþýl Öcal, M. Bertan Yýlmaz, Aykut Pelit, Fatma Çoban, Bora Taºtekin and Ýbrahim Tabakan
Address : Cukurova Univesity, Faculty of Medicine, Department of Biophysics, Balcalý, 01350 Adana/Turkey.
Submitted Date : 3-08-2018
Accepted Date : 13-02-2019


Type-1 diabetes mellitus is an insulin-dependent autoimmune disease, which is very common in the human populations regardless of gender. The aim of this study was to evaluate the effects of pulsed magnetic field (PMF), a non-invasive procedure, on male and female rats with type 1 diabetes, particularly on weight loss ratios, blood glucose levels and diabetic neuropathy. Before, the experimental groups were divided into three groups as control (C (F or M), diabetes (DM (F or M), controlled diabetes (DM(F)-INS or DM(M)-INS) groups according to their sex differences, then these experimental groups were exposed to magnetic field effect (PMF). The rats in the PMF groups were exposed to the pulsed magnetic field at 50 Hz (1.5 mT intensity) for 1h/5days/month. The body weights and blood glucose levels were measured once a week over a month. Female and male diabetic rats developing diabetic neuropathy were evaluated with thermal (thermal latency) and dynamic (mechanical threshold) plantar tests. After six-weeks of PMF treatment, the weight loss rate and increased blood glucose levels due to diabetes reversed in both female and male diabetic rats upon PMF treatment (p <0.05). In diabetic neuropathic female and male rats, the thermal latency values increased, while the mechanical threshold values   decreased. The reduction in diabetic neuropathic rats were statistically significant in diabetic rats (p <0.05). The increased or decreased mechanical threshold and thermal latency values in diabetic neuropathic rats were statistically significant in only male diabetic rats (p <0.05).  Our studies may imply that the effect of PMF in neuropathic pain is gender dependent further inferring that hormonal mechanisms were also important in PMF dependent regulation. 


Blood glucose level Diabetes Diabetic neuropathy Reflex measurements Weight loss rate.


  1. Aba P.E., Asuzu I.U. (2016). Effect of administration of methanol root bark extract of Cussonia arborea on serum lipid profil and oxidative biomarker parameters in alloxan-induced diabetic rats. Indian Journal of Animal Research.
  2. Bellossi A., Pouvreau-Quilien V., Rocher C., Reulloux M. (1998). Effect of pulsed magnetic fields o triglyceride and cholesterol levels in plasma of rats. Panminerva Med, 40: 276-279.
  3. Bassett C. (1993). Beneficial effects of electromagnetic fields. CellBiochem, 51: 387-393.
  4. Bassett C. (1989). Fundamental and practical aspects of therapeutic uses of pulsed electromagnetic fields (PEMFs). Crit Rev Biomed Eng, 17:451-529.
  5. Blank F.S., Miller M., Nichols J., Smithline H., Crabb G., Pekow P. (2009). Blood glucose measurement in patients with suspected    diabetic ketoacidosis: a comparison of Abbott MediSensePCx point-of-care meter values to reference laboratory values. J. Emerg. Nurs., 35:93-6. 
  6. Cañedo-Dorantes L., Soenksen L.R., García-Sánchez C., Trejo-Núñez D., Pérez-Chávez F., Guerrero A., Cardona-Vicario M, García-    Lara C., Collí-Magaña D., Serrano-Luna G., Angeles Chimal J.S., Cabrera G. (2015). Efficacy and safety evaluation of systemic extremely low frequency magnetic fields used in the healing of diabetic foot ulcers—phase II data. Arch Med Res., 46:470-478.
  7. Cevik A., Aydin M., Timurkaan N., Apaydin A.M., Yuksel M. (2017). Pathological and immunohistochemical effects of electromagnetic fields on rat liver. Indian Journal of Animal Research, 51:1134-1137.
  8. Gorczynska E., Wegrzynowicz R. (1991). Glucose homeostatis in rats exposed to magnetic fields. Invest Radiol, 26:1095-1100.
  9. Goudarzi I., Hajizadeh S., Salmani M.E., Abrari K. (2010). Pulsed electromagnetic fields accelerate wound healing in the skin of diabetic rats. Bioelectromagnetics, 31:318-323.
  10. Kannan P.,Vijayaraj A., Sesh P.S.L., Narayanan V., Thangavel A., Pandiyan V. (2016). Antioxidant status in streptozotocin induced diabetic rat streated with vanadium complex. Indian Journal of Animal Research., 50:57-62.
  11. Khajuria P., Raghuwanshi P., Rastogi A., Koul A.L., Zargar R., Kour S. (2018). Hepatoprotective effect of seabuckthorn leaf extract in streptozotocin induced diabetes mellitus in Wistar rats. Indian Journal of Animal Research. 52:1745-1750.
  12. Laiti-Kobierska A., Cieslar G., Sieron A., Grzybek H. (2002). Influence of alternating extremely low frequency ELF magnetic field on structure and function of pancreas in rats. Bioelectromagnetics., 23:49-58.
  13. Lei T., Jing D., Xie K., Jiang M., Li F., et al. (2013). Therapeutic effects of 15 Hz pulsed electromagnetic field on diabetic peripheral neuropathy in streptozotocin-treated rats. PLoS ONE, 8: e61414.
  14. Li S., Yu B., Zhou D., He C., Zhuo Q., Hulme JM. (2013). Electromagnetic fields for treating osteoarthritis. Cochrane Database Syst Rev. 14;(12):CD003523.
  15. Macias M.Y., Battocletti J.H., Sutton C.H., Pintar F.A., Maiman D.J. (2000). Directed and enhanced neurite growth with pulsed magnetic field stimulation. Bioelectromagnetics, 21:272–286.
  16. Mert T., Gunay I., Ocal I. (2010). Neurobiological effects of pulsed magnetic field on diabetes-induced neuropathy. Bioelectromagnetics, 31:39 – 47. 
  17. Navratil L., Hlavaty V., Landsingerova A. (1993). Possible therapeutic applications of pulsed magnetic fields. Cas. Lek. Cesk, 132:590-594.
  18. O’Brien, P.D., Hur, J., Robell, N.J., Hayes, J.M. Sakowski, S.A., Feldman, E.L. (2016). Gender-specific differences in diabetic neuropathy in BTBR ob/ob mice. Journal of Diabetes and Its Complications, 30(1):30-7. 
  19. Ocal I., Kalkan T., Gunay I. (2008). Effects of alternating magnetic field on the metabolism of the healthy and diabetic organisms, Brazilian Archives of Biology and Technology, 51: 523-530.
  20. Pieber K., Herceg M., Paternostro-Sluga T. (2010). Electrotherapy for the treatment of painful diabetic peripheral neuropathy: a review. J Rehabil Med., 42:289–295.
  21. Quittan M., Schuhfrid O., Wiesinger G.F., Fialka – Moser V. (2000). Clinical effectiveness of magnetic field therapy-a review of the literature. Acta. Med. Austriaca, 27:61-68.
  22. Schmader K.E. (2002). Epidemiology and impact on quality of life of postherpetic neuralgia and painful diabetic neuropathy. Clin J Pain, 18: 350–354.
  23. Sima A.A., Kamiya H. (2006). Diabetic neuropathy differs in type 1 and type 2 diabetes. Ann N Y Acad Sci, 1084:235–249.
  24. Sisken B.F., Walker J., Orgel M. (1993). Prospects on clinical application of electrical stimulation for nerve regeneration. J Cellular Biochem, 52:404-409,
  25. Tartakoff A.M. (1980). The Golgi complex: crossroads for vesicular traffic. Int Rev Exp Pathol, 22:227-251.
  26. Tasset I., Medina F.J., Jimena I., Aguera E., Gascon F., et al. (2012). Neuroprotective effects of extremely low-frequency electromagnetic fields on a Huntington’s disease rat model: effects on neurotrophic factors and neuronal density. Neuroscience, 209:54–63.
  27. Walker J., Evans J.M., Resig P., Guarnieri S., Meade P., Sisken B.F. (1994). Enhancement of functional recovery following crush lesion to the rat sciatic nerve by exposure to PMF. Exp Neurol, 125:302-304. 

Global Footprints