Molecular cloning, characterization and tissue specificity of the expression in the TAC1 genes from Henan Huai goat (Capra hircus)

DOI: 10.18805/ijar.B-1025    | Article Id: B-1025 | Page : 679-684
Citation :- Molecular cloning, characterization and tissue specificity of the expression in the TAC1 genes from Henan Huai goat (Capra hircus).Indian Journal Of Animal Research.2020.(54):679-684
Zhilong Tian, Yuqin Wang, Huibin Shi, Zhibo Wu, Xiaohui Zhang, Jianping Wang, Yuanxiao Li, Fang Yang, Yumei Liu and Mingxing Chu
  tianzl0515@163.com
Address : College of Animal Science and Technology,
Henan University of Science and Technology, Luoyang-471 003, China.
Submitted Date : 31-07-2018
Accepted Date : 30-04-2019

Abstract

To further to understand the structure and function of the TAC1 gene, we cloned the full-length cDNAs of the TAC1 genes from goat by rapid amplification of cDNA ends-PCR and the qRT-PCR was used to analyze the TAC1 mRNA expression patterns of goat various tissues. The full-length cDNA of goat TAC1 was 1176 bp, with a 339 bp open reading frame encoding 112 amino acids. The amino acid sequence analysis revealed that goat TAC1 gene encoded a water-drain protein and its relative molecular weight and isoelectric point was 13,012.86 Da and 6.29 respectively. Alignment and phylogenetic analyses revealed that their amino acid sequences were highly similar to those of other vertebrates. TAC1 expression of the goat of the brain, cerebellum, medulla oblongata, heart, liver, spleen, lung, kidney, uterus, ovaries. These results serve as a foundation for further study on the Capra hircus TAC1 gene.

Keywords

Clone and sequence analysis Expression Goat Tachykinin.

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