Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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Indian Journal of Animal Research, volume 54 issue 4 (april 2020) : 456-461

Detection of Bovine Ephemeral Fever Virus and Its Effects on Blood Parameters and Serum Calcium Levels in Cattle Population of District Swabi, Pakistan

Sahibzada Waheed Abdullah, Muti -Ur- Rehman Khan, Asim Aslam, Saima Masood, Amir Ghafoor Bajwa, Ali Ahmed Sheikh
1Department of Pathology, Faculty of Veterinary Sciences, University of Veterinary and Animal Sciences, Lahore-54000, Punjab, Pakistan.
Cite article:- Abdullah Waheed Sahibzada, Khan Rehman -Ur- Muti, Aslam Asim, Masood Saima, Bajwa Ghafoor Amir, Sheikh Ahmed Ali (2020). Detection of Bovine Ephemeral Fever Virus and Its Effects on Blood Parameters and Serum Calcium Levels in Cattle Population of District Swabi, Pakistan. Indian Journal of Animal Research. 54(4): 456-461. doi: 10.18805/ijar.B-1019.
Bovine ephemeral fever (BEF) is a fatal viral disease predominantly affecting cattle and buffaloes. Infection results in a huge economic loss, especially due to the reduction in milk production. In Pakistan. There is a dearth of information on bovine ephemeral fever. The study was designed to detect and examine the effect of bovine ephemeral fever virus on hematological parameters among cattle in Swabi district, Khyber Pakhtunkhwa, Pakistan. A total of fifty blood samples were collected from suspected cattle and scrutinized for bovine ephemeral fever virus, using nested Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Hematological parameters were analyzed using hematology and chemistry analyzer. Of the 50 blood samples, 33 representing 66% were positive for bovine ephemeral fever virus through RT-PCR. A product size of 809 bp was observed in the reaction-I, while 505bp was obtained in reaction-II. Neutrophils of infected cattle were significantly increased (10.44 ± 1.87 × 109/L) (p < 0.05), with a significant decrease (p < 0.05) in lymphocytes levels (2.90 ± 0.97 × 109/L). Changes in other blood parameters were non-significant. Furthermore, there was also a significant decrease (p < 0.05) in serum calcium level (7.84 ± 0.16 mg/dL). We confirmed the detection of bovine ephemeral fever virus by nested reverse transcriptase polymerase chain reaction in Swabi, Khyber Pakhtunkhwa, Pakistan.
  1. Bakhshesh, M. and Abdollahi, D. (2015). Bovine ephemeral fever in Iran: Diagnosis, isolation and molecular characterization. Journal of Arthropod. Borne. Diseases. 9: 195.
  2. Becker, M., Moritz, A. and Giger, U. (2008). Comparative clinical study of canine and feline total blood cell count results with seven in clinic and two commercial laboratory hematology analyzers. Veterinary Clinical Pathology. 37: 373-384.
  3. Davies, F., Ochieng, P. and Walker, A. (1990). The occurrence of ephemeral fever in Kenya, 1968–1988. Veterinary Microbiology. 22: 129-136.
  4. Davies, F., Shaw, T. and Ochieng, P. (1975). Observations on the epidemiology of ephemeral fever in Kenya. Journal of Hygine-Cambridge. 75: 231-235.
  5. Degheidy, Ns., Hassan, H., El-Sanousi, A., Salem, S., Beshir, E., El-Sadawy H.A., Köfer, J. Schobesberger, H. (2011). Recent trials for diagnosis of bovine ephemeral fever in Egypt. In: Animal Hygiene and Sustainable Livestock Production. Proceedings of the XVth International Congress of the International Society for Animal Hygiene, Vienna, Austria. 1: 447-451.
  6. Finlaison, D., Read, A., Zhang, J., Paskin, R. Kirkland, P. (2014). Application of a real time polymerase chain reaction assay to the diagnosis of bovine ephemeral fever during an outbreak in New South Wales and northern Victoria in 2009–10. Australian Veterinary Journal. 92: 24-27.
  7. Gray, E. S., Madiga, M. C., Hermanus, T., Moore, P. L., Wibmer, C. K., Tumba, N. L., Werner, et al. (2011). HIV-1 neutralization breadth develops incrementally over 4 years and is associated with CD4+ T cell decline and high viral load during acute infection. Journal of virology. 85: 4828–4840.
  8. Higbie, Ct., Eshar, D. and Bello, NM. (2015). Evaluation of three point-of-care meters and a portable veterinary chemistry analyzer for measurement of blood glucose concentrations in black-tailed prairie dogs (Cynomys ludovicianus). American Journal of Veterinary Research. 76: 532-539.
  9. Hughes, A. D., Mattison, J., Powderly, J. D., Greene, B. T. King, M. R. (2012). Rapid isolation of viable circulating tumor cells from patient blood samples. Journal of Visualized Experiments: JoVE. 64: 4248.
  10. Jung, U., Jiang, X., Kaufmann, S. H. Patzel, V. (2013). A universal TaqMan-based RT-PCR protocol for cost-efficient detection of small noncoding RNA. RNA. 19: 1-10.
  11. Kirkland, PD. (2002). Akabane and bovine ephemeral fever virus infections. Veterinary Clinics of North America,-Food Animal Practice. 18: 501-514.
  12. Leno, B., Martens, E., Felippe, M., Zanzalari, K., Lawrence, J. Overton, T. (2017). Relationship between methods for measurement of serum electrolytes and the relationship between ionized and total calcium and neutrophil oxidative burst activity in early postpartum dairy cows. Journal of Dairy Science. 100: 9285-9293.
  13. Liao, Y. K., Inaba,Y., LI, N. J., Chain, C. Y., Lee, S. L. and Liou, P.P. (1998). Epidemiology of bovine ephemeral fever virus infection in Taiwan. Microbiology Research. 153: 289-295.
  14. Murphy, G., George, T. St. and Uren, M. (1990). Ephemeral fever-    a biochemical model for inflammatory disease in cattle and sheep. In: Fifth Symposium Arbovirus Research in Australia: Queenland Institute of Medical Research. 268-274.
  15. Rio, DC., Ares, M., Hannon, GJ. Nilsen, TW. (2010). Purification of RNA using TRIzol (TRI reagent) Cold Spring Harbor Protocols. DOI:10.1101/pdb.prot5439
  16. Roya, S. (2008). Survey on serological diagnosis of bovine ephemeral fever (BEF) by IR-BK and Vero cell line in southern provinces of Iran by in vitro method. In: FAVA-OIE Joint Symposium on Emerging Diseases, Bangkok. 267-268.
  17. ST George, T., Cybinski, D., Murphy, G. Dimmock, CK. (1984). Serological and biochemical factors in bovine ephemeral fever. Australian Journal of Biological Sciences. 37: 341-350.
  18. Thabet, NS., Ghazy, E.W., Nayel, M.A. and Abo-Elkhair, M. (2011). Molecular and biochemical studies on bovine ephemeral fever. Research Opinions in Animal and Veterinary Sciencese. 1: 269-275.
  19. Uren, M. and Murphy, G. (1985). Studies on the pathogenesis of bovine ephemeral fever in sentinel cattle. II. Haematological and biochemical data. Veterinary Microbiology. 10: 505-515.
  20. Uren, M., George, Ts. and Murphy, G. (1992). Studies on the pathogenesis of bovine ephemeral fever in experimental cattle III. Virological and biochemical data. Veterinary Microbiology. 30: 297-307.
  21. Valli, V., Hulland, T., Mcsherry, B., Robinson, G. and Gilman, J. (1971). The kinetics of haematopoiesis in the calf. I. An autoradiographical study of myelopoiesis in normal, anaemic and endotoxin treated calves. Research in Veterinary Science. 12: 535.
  22. Walker, P. (2005). Bovine ephemeral fever in Australia and the world. In: The World of Rhabdoviruses: Springer. 292: 57-80.
  23. Walker, Pj., Blasdell, K., Joubert, A., Dietzgen, R. Kuzmin, I. (2012). Ephemeroviruses: arthropod-borne rhabdoviruses of ruminants, with large and complex genomes. Rhabdoviruses: molecular taxonomy, evolution, genomics, ecology, cytopathology and control. 59-88.
  24. Young, P. and Spradbrow, P. (1985). Transmission of virus from serosal fluids and demonstration of antigen in neutrophils and mesothelial cells of cattle infected with bovine ephemeral fever virus. Veterinary Microbiology. 10: 199-207.
  25. Zaher, K. and Ahmed, W. (2011). Investigation of bovine Ephemeral Fever virus in Egyptian cows and buffaloes with emphasis on isolation and identification of a field strain. Golbal Veterinaria. 6: 447-452.

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