Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 45 issue 4 (december 2011) : 295 - 299

PROGESTERONE AND ESTRADIOL PROFILES OF CO-ADMINISTRATION OF EXOGENOUS PROGESTERONE AND ESTRADIOL BENZOATE DURING ESTRUS SYNCHRONIZATION IN COWS

S
Syafnir
N
Noraida
N
N.H. Hashida*
T
T. Normala
Z
Z. Hassan
M
M. Fuad
A
A.R. Hazudi
1Centre for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

  • Submitted|

  • First Online |

  • doi

Cite article:- Syafnir, Noraida, Hashida* N.H., Normala T., Hassan Z., Fuad M., Hazudi A.R. (2025). PROGESTERONE AND ESTRADIOL PROFILES OF CO-ADMINISTRATION OF EXOGENOUS PROGESTERONE AND ESTRADIOL BENZOATE DURING ESTRUS SYNCHRONIZATION IN COWS. Indian Journal of Animal Research. 45(4): 295 - 299. doi: .

ABSTRACT

This study was conducted to assess the effectiveness of estrous synchronization method in cow and to document the profile of progesterone and estradiol levels in three breed (Charolais, Brahman and local indigenous, Kedah-Kelantan) of cows. Twenty eight cycling cows received CIDR (controlled intravaginal drug release) insertion containing 1.38 g of progesterone with 10 mg estradiol benzoate (Day 0). The cows were given 2 mg i.m injection of estradiol benzoate (EB) 24 hours after CIDR removal (Day 8). A single insemination was carried out to each of the synchronized cow using rectovaginal method, 24 hours after EB injection (Day 9). Pregnancy diagnosis was carried out on day 60 post-insemination using rectal palpation. Blood samples were collected on day 0, 3, 7, 8, 9, 12 and 14 after CIDR insertion. Collected blood samples were centrifuged at 2,000 r.p.m for 10 minutes and blood plasma was stored at -20°C until assayed. Concentrations of progesterone and estradiol in blood plasma were assayed using RIA kits. Progesterone reached the highest level on day 3, 9.03 ng/ml (Brahman) and 27.16 ng/ml (Kedah-Kelantan). However, after CIDR removal on day 7, progesterone level continued to decrease and reached the lowest levels, 2.12 ng/ml (Charolais), 0.53 ng/ml (Brahman), and 0.47 ng/ml (Kedah-Kelantan). Insertion of CIDR (Day 0) caused a slight drop in estradiol levels in Charolais (0.00 pg/ml), Brahman (5.01 pg/ml) and Kedah-Kelantan (4.60 pg/ml) cows. Estradiol level reached the highest level, 77.26 pg/ml (Charolais), 34.20 pg/ml (Brahman) and 27.43 pg/ml (Kedah-Kelantan) after estradiol benzoate injection on day 8. It was concluded that synchronization method in the present study managed to give good percentage of pregnancy and similar pattern of progesterone and estradiol profiles regardless breed of the cows.

REFERENCES

  1. Bearden, H.J. and Fuquay, J.W., (2002) Applied animal Reproduction. Fifth edition. Prentice Hall. Upper Saddle River. New Jersey.
  2. Bo, G. A., Adam, G.P. and Mapletoft, R.J., (1995) Exogenous control of follicular wave emergence in cattle. Theriogenology. 43 : 31 – 40.
  3. Cavalieri, J., Hepworth, G., Smart, V.M., Ryan, M. and Macmillan, K.L., (2007) Reproductive performance of lactating dairy cows and heifers resynchronized for a second insemination with an intravaginal progesterone-releasing device for 7 or 8 d with estradiol benzoate injected at the time of device insertion and 24 h after removal. Theriogenology. 67 : 824-834.
  4. Carvalho, J.B., Carvalho, N.A., Reis, E.L., Nichi, M., Souza, A.H. and Baruselli, P.S., (2008) Effect of early luteolysis in progesterone-based timed AI protocols in Bos taurus heifers. Theriogenology. 69 : 167-175.
  5. Jainudeen, M. R., Wahid, H. and Hafez, E. S. E., (2000) Ovulation induction, embryo production and transfer. In:.Reproduction in Farm Animals 7th Edition. Hafez B.. and (Hafez E. S. E. (editor)) Williams and Wilkins. Lippincott. pp. 405 – 430.
  6. Jochle, W., (1993) Forty years of control of the estrus cycle in ruminants: progress made, unresolved problems and the potential impact of sperm encapsulation technology. Reprod Fertil Dev. 5 : 587 – 594.
  7. Kim, I-H. and Kim, U-H., (2007) Comparison of the effect of estradiol benzoate plus progesterone and GnRH on the follicular wave emergence and subsequent follicular development in CIDR-treated, lactating dairy cows with follicular cysts. Anim Reprod Sci. 98 : 197- 203.
  8. Kuroiwa, T., Ishibashi, A., Fukuda, M., Kim, S., Tanaka, T. and Kamomae, H., (2005) Estrus synchronization and conception rate after a progesterone releasing intravaginal device (PRID) treatment from the early luteal phase in heifers. J. Reprod Dev. 51 : 669-673.
  9. Nasser, L.F., Sá Filho, M.F., Reis, E.L., Rezende, C.R., Mapletoft, R.J., Bó, G.A. and Baruselli, P.S., 2011. Exogenous progesterone enhances ova and embryo quality following superovulation of the first follicular wave in Nelore (Bos indicus) donors. Theriogenology. In press, corrected proof.
  10. Ryan, D.P., Galvin, J.A. and O’Farrell, K.J., (1999) Comparison of oestrus synchronization regimens for lactating dairy cows. Anim Reprod Sci. 56 : 153- 168.
  11. Shaham-Albalancy, A., Rosenberg, M., Folman, Y., Graber, Y., Meidan, R. and Wolfenson, D., (2000) Two methods of inducing low plasma progesterone concentrations have different effects on dominant follicle in cows. J. Dairy Sci. 83 : 2771-2778.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 45 issue 4 (december 2011) : 295 - 299

    PROGESTERONE AND ESTRADIOL PROFILES OF CO-ADMINISTRATION OF EXOGENOUS PROGESTERONE AND ESTRADIOL BENZOATE DURING ESTRUS SYNCHRONIZATION IN COWS

    S
    Syafnir
    N
    Noraida
    N
    N.H. Hashida*
    T
    T. Normala
    Z
    Z. Hassan
    M
    M. Fuad
    A
    A.R. Hazudi
    1Centre for Foundation Studies in Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

    • Submitted|

    • First Online |

    • doi

    Cite article:- Syafnir, Noraida, Hashida* N.H., Normala T., Hassan Z., Fuad M., Hazudi A.R. (2025). PROGESTERONE AND ESTRADIOL PROFILES OF CO-ADMINISTRATION OF EXOGENOUS PROGESTERONE AND ESTRADIOL BENZOATE DURING ESTRUS SYNCHRONIZATION IN COWS. Indian Journal of Animal Research. 45(4): 295 - 299. doi: .

    ABSTRACT

    This study was conducted to assess the effectiveness of estrous synchronization method in cow and to document the profile of progesterone and estradiol levels in three breed (Charolais, Brahman and local indigenous, Kedah-Kelantan) of cows. Twenty eight cycling cows received CIDR (controlled intravaginal drug release) insertion containing 1.38 g of progesterone with 10 mg estradiol benzoate (Day 0). The cows were given 2 mg i.m injection of estradiol benzoate (EB) 24 hours after CIDR removal (Day 8). A single insemination was carried out to each of the synchronized cow using rectovaginal method, 24 hours after EB injection (Day 9). Pregnancy diagnosis was carried out on day 60 post-insemination using rectal palpation. Blood samples were collected on day 0, 3, 7, 8, 9, 12 and 14 after CIDR insertion. Collected blood samples were centrifuged at 2,000 r.p.m for 10 minutes and blood plasma was stored at -20°C until assayed. Concentrations of progesterone and estradiol in blood plasma were assayed using RIA kits. Progesterone reached the highest level on day 3, 9.03 ng/ml (Brahman) and 27.16 ng/ml (Kedah-Kelantan). However, after CIDR removal on day 7, progesterone level continued to decrease and reached the lowest levels, 2.12 ng/ml (Charolais), 0.53 ng/ml (Brahman), and 0.47 ng/ml (Kedah-Kelantan). Insertion of CIDR (Day 0) caused a slight drop in estradiol levels in Charolais (0.00 pg/ml), Brahman (5.01 pg/ml) and Kedah-Kelantan (4.60 pg/ml) cows. Estradiol level reached the highest level, 77.26 pg/ml (Charolais), 34.20 pg/ml (Brahman) and 27.43 pg/ml (Kedah-Kelantan) after estradiol benzoate injection on day 8. It was concluded that synchronization method in the present study managed to give good percentage of pregnancy and similar pattern of progesterone and estradiol profiles regardless breed of the cows.

    REFERENCES

    1. Bearden, H.J. and Fuquay, J.W., (2002) Applied animal Reproduction. Fifth edition. Prentice Hall. Upper Saddle River. New Jersey.
    2. Bo, G. A., Adam, G.P. and Mapletoft, R.J., (1995) Exogenous control of follicular wave emergence in cattle. Theriogenology. 43 : 31 – 40.
    3. Cavalieri, J., Hepworth, G., Smart, V.M., Ryan, M. and Macmillan, K.L., (2007) Reproductive performance of lactating dairy cows and heifers resynchronized for a second insemination with an intravaginal progesterone-releasing device for 7 or 8 d with estradiol benzoate injected at the time of device insertion and 24 h after removal. Theriogenology. 67 : 824-834.
    4. Carvalho, J.B., Carvalho, N.A., Reis, E.L., Nichi, M., Souza, A.H. and Baruselli, P.S., (2008) Effect of early luteolysis in progesterone-based timed AI protocols in Bos taurus heifers. Theriogenology. 69 : 167-175.
    5. Jainudeen, M. R., Wahid, H. and Hafez, E. S. E., (2000) Ovulation induction, embryo production and transfer. In:.Reproduction in Farm Animals 7th Edition. Hafez B.. and (Hafez E. S. E. (editor)) Williams and Wilkins. Lippincott. pp. 405 – 430.
    6. Jochle, W., (1993) Forty years of control of the estrus cycle in ruminants: progress made, unresolved problems and the potential impact of sperm encapsulation technology. Reprod Fertil Dev. 5 : 587 – 594.
    7. Kim, I-H. and Kim, U-H., (2007) Comparison of the effect of estradiol benzoate plus progesterone and GnRH on the follicular wave emergence and subsequent follicular development in CIDR-treated, lactating dairy cows with follicular cysts. Anim Reprod Sci. 98 : 197- 203.
    8. Kuroiwa, T., Ishibashi, A., Fukuda, M., Kim, S., Tanaka, T. and Kamomae, H., (2005) Estrus synchronization and conception rate after a progesterone releasing intravaginal device (PRID) treatment from the early luteal phase in heifers. J. Reprod Dev. 51 : 669-673.
    9. Nasser, L.F., Sá Filho, M.F., Reis, E.L., Rezende, C.R., Mapletoft, R.J., Bó, G.A. and Baruselli, P.S., 2011. Exogenous progesterone enhances ova and embryo quality following superovulation of the first follicular wave in Nelore (Bos indicus) donors. Theriogenology. In press, corrected proof.
    10. Ryan, D.P., Galvin, J.A. and O’Farrell, K.J., (1999) Comparison of oestrus synchronization regimens for lactating dairy cows. Anim Reprod Sci. 56 : 153- 168.
    11. Shaham-Albalancy, A., Rosenberg, M., Folman, Y., Graber, Y., Meidan, R. and Wolfenson, D., (2000) Two methods of inducing low plasma progesterone concentrations have different effects on dominant follicle in cows. J. Dairy Sci. 83 : 2771-2778.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)