Article Id: ARCC802 | Page : 174 - 179
Sanjib Kr. Das* and Koutilya Bhattacharjee sanjibkrd@gmail.com
Address : Immunology and Cell Biology Laboratory, Post Graduate Department of Zoology Maulana Azad College, Kolkata 700 013, India


Duttaphrynus himalayanus is an atypical amphibian species, adapted to high altitude and cold climatic conditions of Eastern Himalaya. The peripheral blood leucocytes from D. himalayanus were studied by transmission electron microscopy. The ultra structure and sub cellular organizational architecture of the leukocytes appears to be very similar to mammalian leukocyte and accordingly have been characterized into five different types.


Ultrastructure Duttaphrynus himalayanus Transmission electron microscopy WBC Lymphocytes.


  1. Anderson J S, Reisz R R, Scott D, Fro¨bisch N B & Sumida S S. (2008) A stem batrachian from the Early Permian of Texas and the origin of frogs and salamanders, Nature, 453: 515-518.
  2. Bhattacharjee K & Das S K. (2008) Surface morphology of immunocompetent cells isolated from spleen of Bufo himalayanus (Günther). Indian J Exp Biol, 46: 191-195.
  3. Blomberg B, Bernard C C A & Dutton R W. (1980) In vitro evidence for T –B lymphocyte collaboration in the clawed toad, Xenopus. Eur J Immunol, 10: 869-876.
  4. Cieutat A M, Lobel P, August J T, Kjeldsen L, Sengeløv H, Borregaard N and Bainton D F. (1998) Azurophilic Granules of Human Neutrophilic Leukocytes Are Deficient in Lysosome-Associated Membrane Proteins but Retain the Mannose 6-Phosphate Recognition Marker, Blood, 91: 1044-1058.
  5. Das S K & Chakravarty A. (1998) Tumor Infiltrating Lymphocytes from murine sarcoma: Enrichment and proliferative response. Sci Cult, 64: 50-53.
  6. Das S K & Sarkar K. (2005) A new method for isolation of leukocytes from the peripheral blood of amphibians Bufo himalayanus, (Gunther) and study of their surface morphology by scanning electron microscopy, Indian J Exp Biol, 43: 488-492.
  7. Du Pasquier L, Schwager J, & Flajnik M F. (1989) The immune system of Xenopus, Ann Rev Immunol, 7: 251-275.
  8. Forbes, M R, Mcruer D L & Shutler D. (2006) White blood cell profiles of breeding American toads (Bufo americanus) relative to sex and body size, Comp Clin Pathol, 15: 155-159.
  9. Gentz E J. (2007) Medicine and surgery of amphibians, ILAR Jour, 48: 255-259.
  10. Gleich G J, Loegering D A & Maldonado J E. (1973) Identification of a major basic protein in guinea pig eosinophil granules. J. Exp. Med. 137: 1459-1471.
  11. Global amphibian assessment report 2008, [Species Survival Commission (SSC), International union for Conservation of Nature and Natural Resources (IUCN)].
  12. Hirsch J G & Fedorko M E. (1968) Ultrastructure of human leukocytes after simultaneous fixation with Glutaraldehyde and osmium tetroxide and “postfixation” in uranyl acetate, J Cell Biol, 38: 615-627.
  13. Klaur G G, Humphrey J G, Kunkl A & Dongworth D W. (1980) The follicular dendritic cell: its role in antigen presentation in the generation of immunological memory. Immunol Rev, 53: 3-28.
  14. Mueller J, Brun Del Re G, Buerki H., Keller H U, Hess M W & Cottier H. (1975) Nonspecific acid esterase activity: a criterion for differentiation of T and B lymphocytes in mouse lymph nodes, Eur J Immunol, 5: 270-274.
  15. Nethanel T, Kinsky R, Moav N, Brown R, Ran M & With I P. (1981) Separation of tumor-seeking small lymphocytes and tumor cells using Percoll velocity gradients. J Immunol Met, 41: 43-59.
  16. Opstelten D, Deenen G J, Bos L, Hermes A, Murman L & Nieuwehuis P. (1982) Rapid large scale fraction of lymphoid cells by velocity sedimentation at unit gravity, using percoll as separation medium. J Immunol Met, 48: 189-198.
  17. Pertoft H, Rubin K, Kjellen L, Laurent T C & Klengeborn B. (1977) The viability of cells grown or centrifuged in a new density gradient medium, Percoll. Exp Cell Res, 110: 449-457.
  18. Pettey C L & McKinney E C. (1981) Mitogen induced cytotoxicity in the nurse shark, Dev Comp Immunol, 5: 53-64.
  19. Wolfgang E, Klinert F, Labadie J H & Bower W E. (1982) Accessory and stimulating properties of dendritic cells and macrophages isolated from various rat tisues. J Exp Med, 156: 1-19.

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