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volume 46 issue 3 (september 2012) : 298-301

COMPARATIVE STUDY ON THE EFFECT OF BSA AND FCS AS A SUPPLEMENT IN TCM-199 ON THE IN VITRO MATURATION OF BUFFALO OOCYTES

Y
Yosef Deneke1
T
Trilok Nanda2
P
Prem Singh Yadav
1Central Institute for Research on Buffaloes, Hisar- 125 001, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Deneke1 Yosef, Nanda2 Trilok, Yadav Singh Prem (2025). COMPARATIVE STUDY ON THE EFFECT OF BSA AND FCS AS A SUPPLEMENT IN TCM-199 ON THE IN VITRO MATURATION OF BUFFALO OOCYTES . Indian Journal of Animal Research. 46(3): 298-301. doi: .

ABSTRACT

Availability of developmentally competent buffalo oocytes is critical for in vitro embryo production and application of related biotecniques. The objective of the present study was to assess the effect of BSA in place of FCS as maturation media supplement on in vitro maturation buffalo oocytes. Oocytes were aspirated from abattoir ovarian follicles of 2-8mm diameter followed by maturation in TCM-199 supplemented with hCG, PMSG and containing either 0.4% BSA (group-I) or 10% FCS (group-II). Based on cumulus expansion maturation rate was assessed among the two groups, group II showing a significant higher percentage values (89.1±3.5%) as compared to the group I (73.9±4.2%).

REFERENCES

  1. Agrawal S K, and Tomer, O.S. (1998). Reproductive technologies in buffalo. Indian Vet Res. Inst, Izatnagar, UP,India.
  2. Ball, G. D., Feibfried, M. L., Lenz, R.W., Ax RL, Bavister BD, and First NL(1983). Factors affecting successful in vitro fertilization of bovine follicular oocytes. Biol. Reprod., 28: 717-725.
  3. Bavister, B. D., Rose-Hellenkant, T.A., and Pinyo-pomintr T (1992). Development of in vitro matured, in vitro fertilized bovine embryos into morulae and blastocysts in defined medium. Theriogenology., 37: 124-146.
  4. Chauhan, M.S. and Anand,S.R.(1991). In vitro maturation and fertilization of goat oocytes. Indian J.Exp. Biol.,
  5. 29: 105-110.
  6. Chauhan, M. S., Palta, P, Das, S.K., Katoyar, P. K., and Madan, M. L. (1997). Replacement of serum and hormone additives with follicular fluid in the IVM medium: effects on maturation , fertilization and subsequent development of buffalo oocytes in vitro. Theriogenology, 48: 461-469.
  7. Cox, J.F., Hormazabal, J., and Santa Maria, A. (1993). Effect of cumulus on in vitro fertilization of bovine matured oocytes. Theriogenology, 40: 1259-1267.
  8. De Felici, M., and Siracusa, G. (1982). “Spontaneous” hardening of the zona pellucida of mouse oocytes during in vitro culture. Gamete Res., 6: 107-113.
  9. Fukuda, Y., Ichikawa, M., Naito, K.,and Toyada, Y.(1990). Birth of normal calves resulting from bovine oocytes matured, fertilized and cultured with cumulus cells in vitro up to blastocyst stage. Biol. Reprod., 22: 669.
  10. Handrow, R.R., Lenz, R.W. and Ax, R.L.(1982). Structural comparisons among glycosaminoglycans reaction to promote an acrosome in bovine spermatozoa. Biochem. Biophys. Res. Comm., 107: 1326-1332.
  11. Hurtt, AE; Landim-Alvarenga, F., Seidel, G.E.Jr and Squires, E.L. (2000). Vitrification of immature and mature equine and bovine oocytes in an ethylene glycol, ficoll and sucrose solution using open-pulled straws. Theriogenology. 54: 119-128.
  12. Madan, M.L., Cauhan, M.S., Singla, S.K., and Manik R.S. (1994). Pregnancies established from water buffalo (Bubalus bubalis) blastocysts derived from in vitro matured in vitro fertilized oocytes and co-cultured with cumulus cells. Theriogenology, 57:1095-1103.
  13. Nandi, S., Chauhan, M.S. and Palta,P. ( 1998). Effect of cumulus cells and sperm concentration on cleavage rate and subsequent embryonic development of buffalo (Bubalus bubalis) oocytes matured and fertilized in vitro. Theriogenology, 50: 1251-1262.
  14. Nandi,S., Chauhan, M.S. and Palta,P. ( 2000).Effect of corpus luteum on the recovery and developmental potential of buffalo oocytes. Vet. Rec., 147: 580-581.
  15. Nandi S., Ravindranatha M, Gupta, P.S.P., Raghu, H.M. and Sarma, P.V. (2003). Development competence and post- thaw survivability of buffalo embryos produced in vitro: effect of growth factors in oocytes maturation medium and embryo culture system. Theriogenology, 60(9): 1621-1631.
  16. Schroeder, A.C. and Epipig, J.J. (1984). The developmental capacity of mouse oocytes that matured spontaneously in vitro is normal. Dev. Biol., 102: 493-497.
  17. Shamsuddin, M., Larsson, B., and Rodriguez-Martinez, H. (1993). Maturation-related changes in bovine oocytes under different culture conditions. Anim.l Repro. Sci. 31(1): 49-60.
  18. Singh, G., Totey, S.M., and Talwar, G.P. (1989). In vitro fertilization of oocytes matured in vitro. Theriogenology, 91: 2559 (Abstr).
  19. Singh, S. (1997). In vitro maturation and fertilization of buffalo oocytes. M.V.SC. Thesis submitted to CCS, HAU, Hisar, Haryana, India.
  20. Totey, S.M., Singh, G., Taneja, M.,. Pawshe, C.H. and Talwar, G.P. (1992). In vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalus bubalis). J.Reprod. Fertil., 95: 597-607.
  21. Totey, S.M., Pawshe, C.H., and Singh, G.P. (1993 ). In vitro maturation and fertilization of buffalo oocytes (Bubalus bubalis). Effect of media, hormone and sera. Theriogenology, 39: 1153-1171.
  22. Totey, S.M., Dalri, M., Appa Rao, K.B. C., Pawshe, C.H., Taneja,H. and Chillar, R.S. (1996). Differential cleavage and developmental rates and their correlation with cell numbers and sex ratios in buffalo embryos generated in vitro. Theriogenology, 45: 521-533.
  23. Vanderhyden, B.C., and Armstrong, D.T. (1989). Role of cumulus cells and serum on the in vitro maturation, fertilization, and subsequent development of rat oocytes. Biol. Reprod., 40: 720-728.
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    volume 46 issue 3 (september 2012) : 298-301

    COMPARATIVE STUDY ON THE EFFECT OF BSA AND FCS AS A SUPPLEMENT IN TCM-199 ON THE IN VITRO MATURATION OF BUFFALO OOCYTES

    Y
    Yosef Deneke1
    T
    Trilok Nanda2
    P
    Prem Singh Yadav
    1Central Institute for Research on Buffaloes, Hisar- 125 001, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Deneke1 Yosef, Nanda2 Trilok, Yadav Singh Prem (2025). COMPARATIVE STUDY ON THE EFFECT OF BSA AND FCS AS A SUPPLEMENT IN TCM-199 ON THE IN VITRO MATURATION OF BUFFALO OOCYTES . Indian Journal of Animal Research. 46(3): 298-301. doi: .

    ABSTRACT

    Availability of developmentally competent buffalo oocytes is critical for in vitro embryo production and application of related biotecniques. The objective of the present study was to assess the effect of BSA in place of FCS as maturation media supplement on in vitro maturation buffalo oocytes. Oocytes were aspirated from abattoir ovarian follicles of 2-8mm diameter followed by maturation in TCM-199 supplemented with hCG, PMSG and containing either 0.4% BSA (group-I) or 10% FCS (group-II). Based on cumulus expansion maturation rate was assessed among the two groups, group II showing a significant higher percentage values (89.1±3.5%) as compared to the group I (73.9±4.2%).

    REFERENCES

    1. Agrawal S K, and Tomer, O.S. (1998). Reproductive technologies in buffalo. Indian Vet Res. Inst, Izatnagar, UP,India.
    2. Ball, G. D., Feibfried, M. L., Lenz, R.W., Ax RL, Bavister BD, and First NL(1983). Factors affecting successful in vitro fertilization of bovine follicular oocytes. Biol. Reprod., 28: 717-725.
    3. Bavister, B. D., Rose-Hellenkant, T.A., and Pinyo-pomintr T (1992). Development of in vitro matured, in vitro fertilized bovine embryos into morulae and blastocysts in defined medium. Theriogenology., 37: 124-146.
    4. Chauhan, M.S. and Anand,S.R.(1991). In vitro maturation and fertilization of goat oocytes. Indian J.Exp. Biol.,
    5. 29: 105-110.
    6. Chauhan, M. S., Palta, P, Das, S.K., Katoyar, P. K., and Madan, M. L. (1997). Replacement of serum and hormone additives with follicular fluid in the IVM medium: effects on maturation , fertilization and subsequent development of buffalo oocytes in vitro. Theriogenology, 48: 461-469.
    7. Cox, J.F., Hormazabal, J., and Santa Maria, A. (1993). Effect of cumulus on in vitro fertilization of bovine matured oocytes. Theriogenology, 40: 1259-1267.
    8. De Felici, M., and Siracusa, G. (1982). “Spontaneous” hardening of the zona pellucida of mouse oocytes during in vitro culture. Gamete Res., 6: 107-113.
    9. Fukuda, Y., Ichikawa, M., Naito, K.,and Toyada, Y.(1990). Birth of normal calves resulting from bovine oocytes matured, fertilized and cultured with cumulus cells in vitro up to blastocyst stage. Biol. Reprod., 22: 669.
    10. Handrow, R.R., Lenz, R.W. and Ax, R.L.(1982). Structural comparisons among glycosaminoglycans reaction to promote an acrosome in bovine spermatozoa. Biochem. Biophys. Res. Comm., 107: 1326-1332.
    11. Hurtt, AE; Landim-Alvarenga, F., Seidel, G.E.Jr and Squires, E.L. (2000). Vitrification of immature and mature equine and bovine oocytes in an ethylene glycol, ficoll and sucrose solution using open-pulled straws. Theriogenology. 54: 119-128.
    12. Madan, M.L., Cauhan, M.S., Singla, S.K., and Manik R.S. (1994). Pregnancies established from water buffalo (Bubalus bubalis) blastocysts derived from in vitro matured in vitro fertilized oocytes and co-cultured with cumulus cells. Theriogenology, 57:1095-1103.
    13. Nandi, S., Chauhan, M.S. and Palta,P. ( 1998). Effect of cumulus cells and sperm concentration on cleavage rate and subsequent embryonic development of buffalo (Bubalus bubalis) oocytes matured and fertilized in vitro. Theriogenology, 50: 1251-1262.
    14. Nandi,S., Chauhan, M.S. and Palta,P. ( 2000).Effect of corpus luteum on the recovery and developmental potential of buffalo oocytes. Vet. Rec., 147: 580-581.
    15. Nandi S., Ravindranatha M, Gupta, P.S.P., Raghu, H.M. and Sarma, P.V. (2003). Development competence and post- thaw survivability of buffalo embryos produced in vitro: effect of growth factors in oocytes maturation medium and embryo culture system. Theriogenology, 60(9): 1621-1631.
    16. Schroeder, A.C. and Epipig, J.J. (1984). The developmental capacity of mouse oocytes that matured spontaneously in vitro is normal. Dev. Biol., 102: 493-497.
    17. Shamsuddin, M., Larsson, B., and Rodriguez-Martinez, H. (1993). Maturation-related changes in bovine oocytes under different culture conditions. Anim.l Repro. Sci. 31(1): 49-60.
    18. Singh, G., Totey, S.M., and Talwar, G.P. (1989). In vitro fertilization of oocytes matured in vitro. Theriogenology, 91: 2559 (Abstr).
    19. Singh, S. (1997). In vitro maturation and fertilization of buffalo oocytes. M.V.SC. Thesis submitted to CCS, HAU, Hisar, Haryana, India.
    20. Totey, S.M., Singh, G., Taneja, M.,. Pawshe, C.H. and Talwar, G.P. (1992). In vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalus bubalis). J.Reprod. Fertil., 95: 597-607.
    21. Totey, S.M., Pawshe, C.H., and Singh, G.P. (1993 ). In vitro maturation and fertilization of buffalo oocytes (Bubalus bubalis). Effect of media, hormone and sera. Theriogenology, 39: 1153-1171.
    22. Totey, S.M., Dalri, M., Appa Rao, K.B. C., Pawshe, C.H., Taneja,H. and Chillar, R.S. (1996). Differential cleavage and developmental rates and their correlation with cell numbers and sex ratios in buffalo embryos generated in vitro. Theriogenology, 45: 521-533.
    23. Vanderhyden, B.C., and Armstrong, D.T. (1989). Role of cumulus cells and serum on the in vitro maturation, fertilization, and subsequent development of rat oocytes. Biol. Reprod., 40: 720-728.
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