Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

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B.S. Pradeep, C. Renukaprasad, Placid E.D. Souza
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1Department of Parasitology, Veterinary College, KVAFSU, Hebbal, Bangalore-560 024, India
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Cite article:- Pradeep B.S., Renukaprasad C., Souza E.D. Placid (2023). EVALUATION OF THE COMMONLY USED ACARICIDES AGAINST DIFFERENT STAGES OF THE CATTLE TICK BOOPHILUS MICROPLUS BY USING DIFFERENT IN VITRO TESTS*. Indian Journal of Animal Research. 46(3): 248 - 252. doi: .
The evaluation of the efficacy of three commonly used acaricides viz. cypermethrin, deltamethrin (0.05, 0.1 and 0.2% concentrations) and amitraz (0.1, 0.2 and 0.3% concentrations) was conducted against the cattle tick Boophilus microplus. Three in vitro methods viz. Tea bag method, filter paper impregnation method and immersion method were used to evaluate the efficacy of the acaricides. Adult immersion test with discriminating doses was also done to know the resistance development if present. Comparison of different in vitro methods was done based on the efficacy against larval stages of the ticks. Among the three acaricides amitraz fared better with no indication of resistance development against B. microplus. Cypermethrin showed 30% and deltamethrin showed 10% resistance against B. microplus. Among different in vitro methods, the immersion method fared better in efficacy followed by tea bag method and filter paper method.
  1. Bagherwal, R.K., Ashok Sharma. and Dhanotiya, R.S., (1994). Studies on the efficacy of deltamethrin against different stages of Hyalomma anatolicum anatolicum. Indian Vet. J., 71: 1073-1076.
  2. Bagherwal, R.K., Sisodia, R.S., Ashok Sharma, Dhanotiya, R.S. and Ghosal, S.B., (1995). In vitro studies on the susceptibility of the tick Hyalomma anatolicum anatolicum to acaricides using F.A.O test kit. Indian Vet. J., 72: 332-335.
  3. Bhatia, H.M., (1974). Ixodicides in animal tick control. Vet. Bull., 44(5): 273-282.
  4. FAO, (1999). Acaricide resistance test kit. Instructions for use. 2ndEdn , World Acaricide Resistance Reference Centre (WARRC)., Berlin. 11: 25-77.
  5. Gladney, W.J., Charles, C. Dawkins. and Drummond, R.O., (1972). Insecticides tested for control of nymphal brown dog ticks by the Tea - bag technique. J. Econ. Entomol., 65: 174-176.
  6. Sabnis, A.M., Jagadish, S., Jahagirdar, H.V. and Jayakumar, K.,(1984). In vitro studies on the biological efficacy of propetamphos against the three host tick Rhipicephalus sanguineus, Indian Vet. J., 61: 560-563.
  7. Shaw, R.D., (1966). Culture of an Organophosphorus – resistant strain of Boophilus microplus (Can) and an assessment of its resistance spectrum. Bull. Ent. Res., 56: 389-405.
  8. SHAILAJA, N., (2006) Status of resistance to acaricides in cattle tick Boophilus microplus of Tamil Nadu. M.V.Sc. Thesis TANUVAS, Chennai.
  9. Sisodia, R.S., Bagherwal, R.K. and Mehta, H.K., (1986). Studies on experimental rearing of ticks (Hyalomma anatolicum anatolicum) and preliminary trials on efficacy of Pestoban on their different stages. Livestock Adviser., XI
  10. (Issue XI):47-50 .
  11. Srivastava, P.S. and Sharma, N.N., (1976). On the rearing of Hyalomma anatolicum anatollicum Koch, 1844 and their infection with Theileria annulata (Dscunowsky & Luhs, 1904) in the laboratory. Indian. J. Anim. Res., 10: 21-26.
  12. STUTI VATSYA. and DAS, S.S., (2004) Acaricidal effects on the biological activities of female Boophilus microplus. J. Vet. Parasitol., 18: 91-92.

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