Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 46 issue 3 (september 2012) : 242-247

ISOLATION, CHARACTERIZATION AND ANTIBIOTIC SENSITIVITY TEST OF PATHOGENIC LISTERIA SPECIES IN LIVESTOCK, POULTRY AND FARM ENVIRONMENT OF ODISHA*

L
Laxmi Narayan Sarangi1
H
H.K. Panda
1Department of Bacteriology and Virology, Odisha Veterinary College, O.U.A.T., Bhubaneswar-751 003, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Sarangi1 Narayan Laxmi, Panda H.K. (2025). ISOLATION, CHARACTERIZATION AND ANTIBIOTIC SENSITIVITY TEST OF PATHOGENIC LISTERIA SPECIES IN LIVESTOCK, POULTRY AND FARM ENVIRONMENT OF ODISHA*. Indian Journal of Animal Research. 46(3): 242-247. doi: .

ABSTRACT

The present investigation was undertaken to study the occurrence of Listeria species in different animal and farms of Odisha. A total of 456 samples including 386 clinical samples and 70 environmental                samples collected from different animals like cattle, sheep, goat, pig and poultry of different farms were screened for presence of Listeria species and 33 Listeria species were isolated which on pathogenicity testing revealed 5 L. monocytogenes and 3 L. ivanovii to be pathogenic.  On biochemical characterization they were identified as L. monocytogenes, L. ivanovii, L. innocua, L. welshimeri, L. seeligeri and L. grayi with prevalence rate of 1.75, 0.65, 1.75, 1.53, 1.31 and 0.21 percent respectively. Out of these isolates 5 L. monocytogenes and 2 L. ivanovii were found to be pathogenic in nature. Similarly prevalence of Listeria in different animals revealed 6.49% from cattle, 10% from sheep, 12.5% from goat, 15.83% from pig and 7.14% from poultry. Screening of environmental samples revealed isolation of 4 Listeria species with sewage having the highest prevalence followed by soil. Antibiotic sensitivity study indicated high susceptibility to ciprofloxacin, livofloxacin, amoxicillin, enrofloxacin where as high resistance were observed against gentamycin, penicillin G, cephadroxil, ceftriaxone, cephotaxim and oxytetracycin.

REFERENCES

  1. Aghi, H.M., Garg, S.R. and Mandokhot, U. (2004). Occurrence of Listeria in animal faecal matter, farmyard manure and sewage. Indian J. Anim. Sci. 74(7):737-738.
  2. Al-Ghazali, M.R and Al-Azzawi, S.K. (1990). Listeria monocytogenes contamination of crops grown on soil treated with sewage sludge cake. J. Appl. Bacteriol. 69:642-647.
  3. Bauer, A.W., Kirby, W.M.M., Sherris, K.C. and Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disc method. Am. J. Clin. Pathol. 45:493-496.
  4. BIS (Bureau of Indian Standards). (1994). Committee Draft, General guidance for the detection of Listeria monocytogenes. Microbiology CD, 11290. 11-12.
  5. Bonardi, S., Brindani, F. and Maggi, E. (2002). Isolation of Listeria monocytogenes and Listeria spp. from pigs at slaughtering in Italy. Annali della Facolta’ di Medicina Veterinaria di Parma. 22:205-210.
  6. Chand, P. and Sadana, J.R. (1999). Outbreak of Listeria ivanovii abortion in sheep in India. Vet. Rec. 145:83-84.
  7. Elezebeth, G., Malik, S.V.S., Chaudhari, S.P. and Barbuddhe, S.B. (2007). The occurrence of Listeria species and antibodies against listeriolysin-O in naturally infected goats. Small Ruminant Res. 67(2-3):173-178.
  8. Giridhar, O.P. and Garg, S.R. (2002). Prevalence of Listeria in animal farms. Indian J. Anim. Sci. 72 (10):847-849.
  9. Kaur, S. and Malik, S.V.S. (2007). Antibiotic sensitivity Patterns of Listeria isolates from spontaneous abortion cases. Indian J. Vet. Pub. Hlth. 5(1):1-7.
  10. Low, J.C. and Donachie, W. (1997). A review of Listeria monocytogenes and listeriosis. The Vet. J. 153:9-29.
  11. Malik, S.V.S., Barbuddhe, S.B. and Chaudhary, S.P. (2002). Listeric infections in man and animals in Indian subcontinent: A review. Tropical Anim. Health Pro. 34(5):359-381.
  12. McClain, D. and Lee, W.H. (1988). Development of USDA-FSIS method for isolation of Listeria monocytogenes from raw meat and poultry. J. AOAC Int. 71:660-664.
  13. McLauchlin, J. (1987). Listeria monocytogenes, recent advances in the taxonomy and epidemiology of listeriosis in human. J. Appl. Bacteriol. 63:1-11.
  14. Menudier, A., Bosiraud, C. and Nicolas, J.A. (1991). Virulence of Listeria monocytogenes serovars and Listeria spp. in experimental infection of mice. J. Food Prot. 54:917-921.
  15. Nigam, P., Katoch, R.C., Verma, S. and Batta, M.K. (1998). Chemotherapeutic sensitivity profile of Listeria species from reproductive disorders of domestic animals. Indian Vet. J. 75:658-659.
  16. Nightingale, K. K., Schukken, Y.H., Nightingale, C.R., Fort, E.D., Ho, A.J., Her, Z., Grohn, T., McDonough, P.L. and Wiedmann, M. (2004). Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment, Appl. Environ. Microbiol. 70:4458–4467.
  17. Njagi, L.W., Mbuthia, P.G., Bebora, L.C., Nyaga, P.N., Minga, U. and Olsen, J.E. (2004). Carrier status for Listeria monocytogenes and other Listeria species in free range farm and market healthy indigenous chickens and ducks. East Afr. Med. J. 81(10):529-533.
  18. Notermans, S., Dufrenne, J., Chakraborty, T., Steinmeyer, S. and Terplant, G. (1991). The chick embryo test agrees with the mouse bio-assay for assessment of the pathogenicity of Listeria species. Lett. Appl. Microbiol. 13:161-164.
  19. Phadke, S.P., Bhagwat, S.V., Kapsikar, R.N. and Gheveri, S.D. (1979). Listeriosis in sheep and goats in Maharastra. Indian Vet. J. 56:634-637.
  20. Parihar, V.S., Barbuddhe, S.B., Chakurkar, E.B., Danielsson-Tham, M.L. and Tham, W. (2007). Isolation of Listeria species from farm bulk milk at the receiving dairy plant and cervico-vaginal swabs. Indian J. Comp. Microbiol. Immunol. Infect. Dis. 28:53-55.
  21. Sarangi, L.N., Panda, H.K., Priyadarshini, A., Sahoo, S., Palai, T.K., Ranabijuli, S., Senapati, S. and Mohanty, D.N. (2009). Prevalence of Listeria specioes in milk samples of cattle of Odisha. Indian J. Comp. Microbiol. Immunol. Infect. Dis. 30(2):135-136.
  22. Schaffter, N. and Parriaux, A. (2002). Pathogenic bacterial water contamination in mountainous catchment. Water Res. 36:131-139.
  23. Schlech, W.F., Lavigne, P.M., Bortolussi, R.A., Allen, A.C., Haldane, E.V., Wort, A. J., Hightower, A.W., Johnson, S.E., King, S.H., Nicholls, E.S. and Broom, C.V. (1983). Epidemic listeriosis- evidence for transmission by food, New Engl. J. Med. 308:203-206.
  24. Skovgaard, N. and Morgen, C.A. (1988). Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin, Skovgaard, N. and Morgen, C.A. (1988). . Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin. Int. J. Food Microbiol. 6:229-242.
  25. Thakur, S. (2000). M.V.Sc. Thesis, Deemed University, Indian Veterinary Research Institute, Izatnagar, U.P. India.
  26. Unnerstad, H., Bannerman, E., Billie, J., Danielsson-Tham, M.L. and Tham, W. (1996). Prolong contamination of a dairy with Listeria monocytogenes. Neth. Milk Dairy J. 50:493-496.
  27. Vijay Krishna. S., Venkata Reddy, T., Varalakshmi, K. and Subrahmanyam, K. V. (2000). Listeriosis in broiler chicken. Indian Vet. J. 77:285-286.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 46 issue 3 (september 2012) : 242-247

    ISOLATION, CHARACTERIZATION AND ANTIBIOTIC SENSITIVITY TEST OF PATHOGENIC LISTERIA SPECIES IN LIVESTOCK, POULTRY AND FARM ENVIRONMENT OF ODISHA*

    L
    Laxmi Narayan Sarangi1
    H
    H.K. Panda
    1Department of Bacteriology and Virology, Odisha Veterinary College, O.U.A.T., Bhubaneswar-751 003, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Sarangi1 Narayan Laxmi, Panda H.K. (2025). ISOLATION, CHARACTERIZATION AND ANTIBIOTIC SENSITIVITY TEST OF PATHOGENIC LISTERIA SPECIES IN LIVESTOCK, POULTRY AND FARM ENVIRONMENT OF ODISHA*. Indian Journal of Animal Research. 46(3): 242-247. doi: .

    ABSTRACT

    The present investigation was undertaken to study the occurrence of Listeria species in different animal and farms of Odisha. A total of 456 samples including 386 clinical samples and 70 environmental                samples collected from different animals like cattle, sheep, goat, pig and poultry of different farms were screened for presence of Listeria species and 33 Listeria species were isolated which on pathogenicity testing revealed 5 L. monocytogenes and 3 L. ivanovii to be pathogenic.  On biochemical characterization they were identified as L. monocytogenes, L. ivanovii, L. innocua, L. welshimeri, L. seeligeri and L. grayi with prevalence rate of 1.75, 0.65, 1.75, 1.53, 1.31 and 0.21 percent respectively. Out of these isolates 5 L. monocytogenes and 2 L. ivanovii were found to be pathogenic in nature. Similarly prevalence of Listeria in different animals revealed 6.49% from cattle, 10% from sheep, 12.5% from goat, 15.83% from pig and 7.14% from poultry. Screening of environmental samples revealed isolation of 4 Listeria species with sewage having the highest prevalence followed by soil. Antibiotic sensitivity study indicated high susceptibility to ciprofloxacin, livofloxacin, amoxicillin, enrofloxacin where as high resistance were observed against gentamycin, penicillin G, cephadroxil, ceftriaxone, cephotaxim and oxytetracycin.

    REFERENCES

    1. Aghi, H.M., Garg, S.R. and Mandokhot, U. (2004). Occurrence of Listeria in animal faecal matter, farmyard manure and sewage. Indian J. Anim. Sci. 74(7):737-738.
    2. Al-Ghazali, M.R and Al-Azzawi, S.K. (1990). Listeria monocytogenes contamination of crops grown on soil treated with sewage sludge cake. J. Appl. Bacteriol. 69:642-647.
    3. Bauer, A.W., Kirby, W.M.M., Sherris, K.C. and Turck, M. (1966). Antibiotic susceptibility testing by a standardized single disc method. Am. J. Clin. Pathol. 45:493-496.
    4. BIS (Bureau of Indian Standards). (1994). Committee Draft, General guidance for the detection of Listeria monocytogenes. Microbiology CD, 11290. 11-12.
    5. Bonardi, S., Brindani, F. and Maggi, E. (2002). Isolation of Listeria monocytogenes and Listeria spp. from pigs at slaughtering in Italy. Annali della Facolta’ di Medicina Veterinaria di Parma. 22:205-210.
    6. Chand, P. and Sadana, J.R. (1999). Outbreak of Listeria ivanovii abortion in sheep in India. Vet. Rec. 145:83-84.
    7. Elezebeth, G., Malik, S.V.S., Chaudhari, S.P. and Barbuddhe, S.B. (2007). The occurrence of Listeria species and antibodies against listeriolysin-O in naturally infected goats. Small Ruminant Res. 67(2-3):173-178.
    8. Giridhar, O.P. and Garg, S.R. (2002). Prevalence of Listeria in animal farms. Indian J. Anim. Sci. 72 (10):847-849.
    9. Kaur, S. and Malik, S.V.S. (2007). Antibiotic sensitivity Patterns of Listeria isolates from spontaneous abortion cases. Indian J. Vet. Pub. Hlth. 5(1):1-7.
    10. Low, J.C. and Donachie, W. (1997). A review of Listeria monocytogenes and listeriosis. The Vet. J. 153:9-29.
    11. Malik, S.V.S., Barbuddhe, S.B. and Chaudhary, S.P. (2002). Listeric infections in man and animals in Indian subcontinent: A review. Tropical Anim. Health Pro. 34(5):359-381.
    12. McClain, D. and Lee, W.H. (1988). Development of USDA-FSIS method for isolation of Listeria monocytogenes from raw meat and poultry. J. AOAC Int. 71:660-664.
    13. McLauchlin, J. (1987). Listeria monocytogenes, recent advances in the taxonomy and epidemiology of listeriosis in human. J. Appl. Bacteriol. 63:1-11.
    14. Menudier, A., Bosiraud, C. and Nicolas, J.A. (1991). Virulence of Listeria monocytogenes serovars and Listeria spp. in experimental infection of mice. J. Food Prot. 54:917-921.
    15. Nigam, P., Katoch, R.C., Verma, S. and Batta, M.K. (1998). Chemotherapeutic sensitivity profile of Listeria species from reproductive disorders of domestic animals. Indian Vet. J. 75:658-659.
    16. Nightingale, K. K., Schukken, Y.H., Nightingale, C.R., Fort, E.D., Ho, A.J., Her, Z., Grohn, T., McDonough, P.L. and Wiedmann, M. (2004). Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment, Appl. Environ. Microbiol. 70:4458–4467.
    17. Njagi, L.W., Mbuthia, P.G., Bebora, L.C., Nyaga, P.N., Minga, U. and Olsen, J.E. (2004). Carrier status for Listeria monocytogenes and other Listeria species in free range farm and market healthy indigenous chickens and ducks. East Afr. Med. J. 81(10):529-533.
    18. Notermans, S., Dufrenne, J., Chakraborty, T., Steinmeyer, S. and Terplant, G. (1991). The chick embryo test agrees with the mouse bio-assay for assessment of the pathogenicity of Listeria species. Lett. Appl. Microbiol. 13:161-164.
    19. Phadke, S.P., Bhagwat, S.V., Kapsikar, R.N. and Gheveri, S.D. (1979). Listeriosis in sheep and goats in Maharastra. Indian Vet. J. 56:634-637.
    20. Parihar, V.S., Barbuddhe, S.B., Chakurkar, E.B., Danielsson-Tham, M.L. and Tham, W. (2007). Isolation of Listeria species from farm bulk milk at the receiving dairy plant and cervico-vaginal swabs. Indian J. Comp. Microbiol. Immunol. Infect. Dis. 28:53-55.
    21. Sarangi, L.N., Panda, H.K., Priyadarshini, A., Sahoo, S., Palai, T.K., Ranabijuli, S., Senapati, S. and Mohanty, D.N. (2009). Prevalence of Listeria specioes in milk samples of cattle of Odisha. Indian J. Comp. Microbiol. Immunol. Infect. Dis. 30(2):135-136.
    22. Schaffter, N. and Parriaux, A. (2002). Pathogenic bacterial water contamination in mountainous catchment. Water Res. 36:131-139.
    23. Schlech, W.F., Lavigne, P.M., Bortolussi, R.A., Allen, A.C., Haldane, E.V., Wort, A. J., Hightower, A.W., Johnson, S.E., King, S.H., Nicholls, E.S. and Broom, C.V. (1983). Epidemic listeriosis- evidence for transmission by food, New Engl. J. Med. 308:203-206.
    24. Skovgaard, N. and Morgen, C.A. (1988). Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin, Skovgaard, N. and Morgen, C.A. (1988). . Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin. Int. J. Food Microbiol. 6:229-242.
    25. Thakur, S. (2000). M.V.Sc. Thesis, Deemed University, Indian Veterinary Research Institute, Izatnagar, U.P. India.
    26. Unnerstad, H., Bannerman, E., Billie, J., Danielsson-Tham, M.L. and Tham, W. (1996). Prolong contamination of a dairy with Listeria monocytogenes. Neth. Milk Dairy J. 50:493-496.
    27. Vijay Krishna. S., Venkata Reddy, T., Varalakshmi, K. and Subrahmanyam, K. V. (2000). Listeriosis in broiler chicken. Indian Vet. J. 77:285-286.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)