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Current IssueEditorial BoardOnline First ArticlesArchive

volume 46 issue 2 (june 2012) : 137 - 142

EFFECT OF FSH ON EXPRESSION OF CATHEPSIN K AND S DURING IN VITRO MATURATION OF BUFFALO OOCYTES AND THEIR SUBSEQUENT DEVELOPMENTAL COMPETENCE

A
Asit Jain*1
2
2
T
Tripti Jain2
G
G.K. Sachdeva1
S
S De
S
S.L. Goswami
T
T.K. Datta
1Animal Genomics Lab, ABTC, National Dairy Research Institute, Karnal-132001, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Jain*1 Asit, 2, Jain2 Tripti, Sachdeva1 G.K., De S, Goswami S.L., Datta T.K. (2025). EFFECT OF FSH ON EXPRESSION OF CATHEPSIN K AND S DURING IN VITRO MATURATION OF BUFFALO OOCYTES AND THEIR SUBSEQUENT DEVELOPMENTAL COMPETENCE. Indian Journal of Animal Research. 46(2): 137 - 142. doi: .

ABSTRACT

Follicle stimulating hormone (FSH) has been identified as a modulator of oocyte function and antiapoptotic molecule. It has been observed that FSH decreased follicular atresia by decreasing the aspartic lysosomal enzyme (cathepsin D) in rodent. In present study, it was determined whether FSH treatment of oocytes during maturation affects expression of cathepsin K and S (cysteine cathepsins) genes, DNA fragmentation during maturation and associated developmental capacity of oocytes. COCs were collected from abattoir derived ovaries and in vitro matured  in TCM-199 based serum-free medium containing 0 (control) and 10 mg/ml FSH at 38.5ºC for 24 hours. After maturation transcript level of cathepsin K and S genes was determined by optimized real time PCR assay in cumulus cells. In vitro fertilization (IVF) and in vitro culture (IVC) of fertilized embryos were carried out in mSOF medium. Terminal deoxynucleotidyl transferase–mediated dUTP nick-end labeling (TUNEL) assay was carried out to count apoptotic cells. It was found that FSH may involve in attenuating cumulus cells apoptosis by decreasing the cathepsin K and S expression, which probably helps in better in-vitro oocytes maturation, in vitro fertilization and further embryonic development.

REFERENCES

  1. Ali, A., Sirard, M.A. (2002). The effects of 17-estradiol and protein supplement on the response to puriûed and recombinant follicle stimulating hormone in bovine oocytes. Zygote, 10: 65–71.
  2. Balboula, A.Z., Yamanaka, K., Sakatani, M., Hegab, A.O., Zaabel, S.M., Takahashi, M. (2010a). Cathepsin B Activity is Related to the Quality of Bovine Cumulus Oocyte Complexes and Its Inhibition Can Improve Their Developmental Competence. Mol Reprod Develop., 77: 439–448.
  3. Balboula, A.Z., Yamanaka, K., Sakatani, M., Hegab, A.O., Zaabel, S.M., Takahashi, M. (2010b). Intracellular Cathepsin B Activity Is Inversely Correlated With the Quality and Developmental Competence of Bovine Preimplantation Embryos. Mole Reprod Devlop., 77, 1031–1039.
  4. Bettegowda, A., Osman, V., Patel, K.B., Lee, K.E., Mohamed, S., Jianbo, Y., James, J.I. George, W.S. (2008). Identification of Novel Bovine Cumulus Cell Molecular Markers Predictive of Oocyte Competence: Functional and Diagnostic Implications. Biol Repro., 79: 301–309.
  5. Chen, L., Russell, P.T., Larsen, W.J. (1993). Functional significance of cumulus expansion in the mouse: roles for the preovulatory synthesis of hyaluronic acid within the cumulus mass. Mol Repro. Dev., 34: 87-93.
  6. Corn, C.M., Hauser-Kronberger, C., Moser, M. (2005). Predictive value of cumulus cell apoptosis with regard to blastocyst development of corresponding gametes. Fertilm Steril., 84: 627–633.
  7. Dhanasekaran, N., Moudgal, N. R. (1988). Gonadotropin Regulation of Rat Ovarian: Existence of a Hormone Specific Dual Control Mechanism. Bioscie Reports, 8:279-285.
  8. Dhanasekaran, N., Rani, C.S.S., Moudgal, N. R. (1983). Studies on follicular atresia: Lysosomal enzyme activity and gonadotropin Receptors of granulosa cells following administration or withdrawal of gonadotropins in the rat. Mol Cell Endocrinol., 33: 97- 112.
  9. Haldar, A., Prakash, B.S. (2007). Effect of exogenous growth-hormone-releasing factor on blood metabolites and minerals in late maturing buffalo heifers (Bubalus bubalis). J Anim Physiol Anim Nutr., 91:326-332.
  10. Host, E., Mikkelsen, A.L., Lindenberg, S., Smidt-Jensen, S. (2000). Apoptosis in human cumulus cells in relation to maturation stage and cleavage of the corresponding oocyte. Acta Obstetricia et Gynecologica Scandinavica, 79: 936–940.
  11. Houseweart, M.K., Vilaythong, A., Yin, X.M., Turk, B., Noebels, J.L., Myers, R. M. (2003). Apoptosis caused by cathepsins does not require Bid signaling in an in vivo model of progressive myoclonus epilepsy (EPM1). Cell Death Differ., 10: 1329-1335.
  12. Hufana-Duran D., Pedro P.B., Venturina, H.V., Duran, P.G. and Cruz, L.C. (2007). Full-term delivery of River buffalo calves (2n = 50) from in vitro-derived vitrified embryos by swamp buffalo recipients (2n = 48). Livest Sci., 107: 213-219.
  13. Izadyar, F., Zeinstra, E., Bevers, M.M. (1998). Follicle-stimulating hormone and growth hormone act differently on nuclear maturation while both enhance developmental competence of in vitro matured bovine oocytes. Mol Reprod Dev., 51: 339–345.
  14. Livak, K.J and Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 25: 402–408.
  15. Singh, B., Armstrong, D.T. (1997). Insulin-like growth factor-1, a component of serum that enables porcine cumulus cells to expand in response to follicle-stimulating hormone in vitro. Biol Reprod., 56: 1370-1375.
  16. Totey, S.M, Pawshe, C.H., Singh, G.P. (1993). In vitro maturation and fertilization of buffalo oocytes (Bubalus bubalis) effects of media hormones and sera. Theriogenology, 39:1153-1171.
  17. Turk, B., Stoka, V. (2007). Protease signalling in cell death: caspases versus cysteine cathepsins. FEBS Lett.,
  18. 581: 2761-2767.
  19. Ye, K.H.S., Campbell, J. Craigon, M.R. (2005). Luck Dynamic Changes in Meiotic Progression and Improvement of Developmental Competence of Pig Oocytes in Vitro by Follicle-Stimulating Hormone and Cycloheximide Bio Reprod., 72: 399–406.
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Indian Journal of Animal Research
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    volume 46 issue 2 (june 2012) : 137 - 142

    EFFECT OF FSH ON EXPRESSION OF CATHEPSIN K AND S DURING IN VITRO MATURATION OF BUFFALO OOCYTES AND THEIR SUBSEQUENT DEVELOPMENTAL COMPETENCE

    A
    Asit Jain*1
    2
    2
    T
    Tripti Jain2
    G
    G.K. Sachdeva1
    S
    S De
    S
    S.L. Goswami
    T
    T.K. Datta
    1Animal Genomics Lab, ABTC, National Dairy Research Institute, Karnal-132001, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Jain*1 Asit, 2, Jain2 Tripti, Sachdeva1 G.K., De S, Goswami S.L., Datta T.K. (2025). EFFECT OF FSH ON EXPRESSION OF CATHEPSIN K AND S DURING IN VITRO MATURATION OF BUFFALO OOCYTES AND THEIR SUBSEQUENT DEVELOPMENTAL COMPETENCE. Indian Journal of Animal Research. 46(2): 137 - 142. doi: .

    ABSTRACT

    Follicle stimulating hormone (FSH) has been identified as a modulator of oocyte function and antiapoptotic molecule. It has been observed that FSH decreased follicular atresia by decreasing the aspartic lysosomal enzyme (cathepsin D) in rodent. In present study, it was determined whether FSH treatment of oocytes during maturation affects expression of cathepsin K and S (cysteine cathepsins) genes, DNA fragmentation during maturation and associated developmental capacity of oocytes. COCs were collected from abattoir derived ovaries and in vitro matured  in TCM-199 based serum-free medium containing 0 (control) and 10 mg/ml FSH at 38.5ºC for 24 hours. After maturation transcript level of cathepsin K and S genes was determined by optimized real time PCR assay in cumulus cells. In vitro fertilization (IVF) and in vitro culture (IVC) of fertilized embryos were carried out in mSOF medium. Terminal deoxynucleotidyl transferase–mediated dUTP nick-end labeling (TUNEL) assay was carried out to count apoptotic cells. It was found that FSH may involve in attenuating cumulus cells apoptosis by decreasing the cathepsin K and S expression, which probably helps in better in-vitro oocytes maturation, in vitro fertilization and further embryonic development.

    REFERENCES

    1. Ali, A., Sirard, M.A. (2002). The effects of 17-estradiol and protein supplement on the response to puriûed and recombinant follicle stimulating hormone in bovine oocytes. Zygote, 10: 65–71.
    2. Balboula, A.Z., Yamanaka, K., Sakatani, M., Hegab, A.O., Zaabel, S.M., Takahashi, M. (2010a). Cathepsin B Activity is Related to the Quality of Bovine Cumulus Oocyte Complexes and Its Inhibition Can Improve Their Developmental Competence. Mol Reprod Develop., 77: 439–448.
    3. Balboula, A.Z., Yamanaka, K., Sakatani, M., Hegab, A.O., Zaabel, S.M., Takahashi, M. (2010b). Intracellular Cathepsin B Activity Is Inversely Correlated With the Quality and Developmental Competence of Bovine Preimplantation Embryos. Mole Reprod Devlop., 77, 1031–1039.
    4. Bettegowda, A., Osman, V., Patel, K.B., Lee, K.E., Mohamed, S., Jianbo, Y., James, J.I. George, W.S. (2008). Identification of Novel Bovine Cumulus Cell Molecular Markers Predictive of Oocyte Competence: Functional and Diagnostic Implications. Biol Repro., 79: 301–309.
    5. Chen, L., Russell, P.T., Larsen, W.J. (1993). Functional significance of cumulus expansion in the mouse: roles for the preovulatory synthesis of hyaluronic acid within the cumulus mass. Mol Repro. Dev., 34: 87-93.
    6. Corn, C.M., Hauser-Kronberger, C., Moser, M. (2005). Predictive value of cumulus cell apoptosis with regard to blastocyst development of corresponding gametes. Fertilm Steril., 84: 627–633.
    7. Dhanasekaran, N., Moudgal, N. R. (1988). Gonadotropin Regulation of Rat Ovarian: Existence of a Hormone Specific Dual Control Mechanism. Bioscie Reports, 8:279-285.
    8. Dhanasekaran, N., Rani, C.S.S., Moudgal, N. R. (1983). Studies on follicular atresia: Lysosomal enzyme activity and gonadotropin Receptors of granulosa cells following administration or withdrawal of gonadotropins in the rat. Mol Cell Endocrinol., 33: 97- 112.
    9. Haldar, A., Prakash, B.S. (2007). Effect of exogenous growth-hormone-releasing factor on blood metabolites and minerals in late maturing buffalo heifers (Bubalus bubalis). J Anim Physiol Anim Nutr., 91:326-332.
    10. Host, E., Mikkelsen, A.L., Lindenberg, S., Smidt-Jensen, S. (2000). Apoptosis in human cumulus cells in relation to maturation stage and cleavage of the corresponding oocyte. Acta Obstetricia et Gynecologica Scandinavica, 79: 936–940.
    11. Houseweart, M.K., Vilaythong, A., Yin, X.M., Turk, B., Noebels, J.L., Myers, R. M. (2003). Apoptosis caused by cathepsins does not require Bid signaling in an in vivo model of progressive myoclonus epilepsy (EPM1). Cell Death Differ., 10: 1329-1335.
    12. Hufana-Duran D., Pedro P.B., Venturina, H.V., Duran, P.G. and Cruz, L.C. (2007). Full-term delivery of River buffalo calves (2n = 50) from in vitro-derived vitrified embryos by swamp buffalo recipients (2n = 48). Livest Sci., 107: 213-219.
    13. Izadyar, F., Zeinstra, E., Bevers, M.M. (1998). Follicle-stimulating hormone and growth hormone act differently on nuclear maturation while both enhance developmental competence of in vitro matured bovine oocytes. Mol Reprod Dev., 51: 339–345.
    14. Livak, K.J and Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 25: 402–408.
    15. Singh, B., Armstrong, D.T. (1997). Insulin-like growth factor-1, a component of serum that enables porcine cumulus cells to expand in response to follicle-stimulating hormone in vitro. Biol Reprod., 56: 1370-1375.
    16. Totey, S.M, Pawshe, C.H., Singh, G.P. (1993). In vitro maturation and fertilization of buffalo oocytes (Bubalus bubalis) effects of media hormones and sera. Theriogenology, 39:1153-1171.
    17. Turk, B., Stoka, V. (2007). Protease signalling in cell death: caspases versus cysteine cathepsins. FEBS Lett.,
    18. 581: 2761-2767.
    19. Ye, K.H.S., Campbell, J. Craigon, M.R. (2005). Luck Dynamic Changes in Meiotic Progression and Improvement of Developmental Competence of Pig Oocytes in Vitro by Follicle-Stimulating Hormone and Cycloheximide Bio Reprod., 72: 399–406.
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