Indian Journal of Animal Research, volume 46 issue 1 (march 2012) : 70 - 73

SERODIAGNOSIS OF CRYPTOSPORIDIOSIS IN CATTLE BY IFAT*

M. Veena, Placid. E. D’Souza, G. S. Sreenivasa Murthy, C. Renuka Prasad**

Affiliations

¹Department of Veterinary Parasitology, Centre of Advanced Faculty Training, KVAFSU, Veterinary College, Hebbal, Bangalore- 560 024, India

Submitted|
First Online |
doi

Cite article:- Veena M., D’Souza E. Placid., Murthy Sreenivasa S. G., Prasad** Renuka C. (2025). SERODIAGNOSIS OF CRYPTOSPORIDIOSIS IN CATTLE BY IFAT*. Indian Journal of Animal Research. 46(1): 70 - 73. doi: .

ABSTRACT

The indirect fluorescent antibody technique was standardised for serodiagnosis of Cryptosporidiosis in naturally infected cattle. A total of 201 faecal and serum samples were collected from different farms, animal shelters and abbatoirs in and around Bangalore which included 11 samples positive for Cryptosporidium infection on faecal microscopy. Two species viz., Cryptosporidium parvum and Cryptosporidium andersoni were prevalent based on faecal examination. Oocysts were purified by using sedimentation and gradient centrifugation and then the samples were screened by immunofluorescence antibody technique (IFAT). Out of 201 sera samples 25 samples were positive for Cryptosporidial antibodies indicating an infection rate of 12.44%.

KEYWORDS

REFERENCES

Arrowood, M. J. and Sterling, C. R., (1987) Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic percoll gradients. J. Parasito.., 78 : 314-319.
Current and N.C. Reese, A comparison of endogenous development of three isolates of Cryptosporidium in suckling mice, J. Protozoology. 33 (1986), pp. 98–108.
Elory, D. M., Laila, H. S. and Gudrun, E., (1987) Cryptosporidium antibodies in Manitoba cattle: A pilot study using an indirect fluorescent antibody procedure. Canadian Vete. J.., 28: 126-128.
Lindsay, D. S., Upton, S. J., Owens, D. S., Morgan, U. M., Mead, J. R. and blagburn, B.L., (2000) Cryptosporidium andersoni n. spp. (Apicomplexa:Cryptosporididae) from cattle, Bos Taurus. J.Eukaryotic Microbiol., 47: 91-95.
Lorenzo, L. M. J., Ares-mazas, E. and Villacorta M. M. I., (1993) Detection of oocysts and IgG antibodies to Cryptosporidium parvum in assymptomatic adult cattle. Vety. Parasito., 41: 9-15.
O’donghue, J., (1995) Cryptosporidium and Cryptosporidiosis in man and animals. Intern. J. Parasitol.., 25 : 139-195.
Scott, C.A., Smith, H.V., Mtambo, M.M.A. and Gibbs, H.A., (1995) An epidemiological study of Cryptosporidium parvum in two herds of adult beef cattle. Vety. Parasito., 57 : 277-288.

Disclaimer :

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

SERODIAGNOSIS OF CRYPTOSPORIDIOSIS IN CATTLE BY IFAT*

ABSTRACT

KEYWORDS

REFERENCES

Reviewed By

In this Article

APC

Publish With US

Become a Reviewer/Member

Open Access

Products and Services

Support and Policies

Editorial Board