Chief Editor:

M. R. Saseendranath

Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA

Frequency:Monthly

Indexing:

Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

COMPARISON OF DETECTION THRESHOLD OF DIFFERENT PASTEURELLA MULTOCIDA SPECIFIC PCRS

Lalsiamthara Jonathan, Anil Kumar Arora

Affiliations

¹College of Veterinary Science, G. A. D. Veterinary and Animal Sci. Univ., Ludhiana-141 004, India

Submitted|
First Online |
doi

Cite article:- Jonathan Lalsiamthara, Arora Kumar Anil (2025). COMPARISON OF DETECTION THRESHOLD OF DIFFERENT PASTEURELLA MULTOCIDA SPECIFIC PCRS. Indian Journal of Animal Research. 46(1): 28-33. doi: .

ABSTRACT

The present study was undertaken to determine the detection threshold of five different Pasteurella multocida PCRs using a standard vaccine strain P52 as DNA template. The purified genomic DNA was diluted ten-fold serially and then subject to polymerase chain reaction. Among the primers assessed, IPFWD or IPREV primers showed the highest sensitivity, detecting purified DNA at 5 fg concentration (~2 bacterial cells). KMT1T7 & KMT1SP6, KTSP61 & KTT72 and PSL-(forward & reverse) primers could detect P52 DNA at the levels of 500, 50 and 50 pg respectively. PM23F1 & PM23R2 primers were the least sensitive and could detect P52 DNA at 5 ng concentration only.

KEYWORDS

REFERENCES

Benkirane A and De Alwis M C L. (2002). Veterinary Medicine - Czech 47: 234-240.
Brickell S K, Thomas L M, Long K A, Panaccio M and Widders P R. (1998). Veterinary Microbiology 59: 295–307.
Kasten R W, Carpenter T E, Snipes K P and Hirsch D C. (1997). Avian Diseases 41: 676-682.
Lee C W, Wilkie I W, Townsend K M and Frost A J. (2000). Veterinary Microbiology 72: 47–55.
Miflin J K and Blackall P J. (2001). Letters in Applied Microbiology 33: 216-221.
Townsend K M, Frost A J, Lee C W, Papadimitriou J M and Dawkins H J S. (1998). Journal of Clinical Microbiology 36: 1096-1100.
Wilson K (1987). Preparation of genomic DNA from bacteria. In: F.M. Ausubal, R. Brent, R.L. Kirston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl (Eds.), Current Protocols in Molecular Biology Vol. 1. John Wiley and Sons, New York pp. 2.4.1-2.4.2.

Disclaimer :

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.