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COMPARISON OF DETECTION THRESHOLD OF DIFFERENT PASTEURELLA MULTOCIDA SPECIFIC PCRS

Article Id: ARCC407 | Page : 28-33
Citation :- COMPARISON OF DETECTION THRESHOLD OF DIFFERENT PASTEURELLA MULTOCIDA SPECIFIC PCRS.Indian Journal of Animal Research.2012.(46):28-33
Lalsiamthara Jonathan and Anil Kumar Arora
Address : College of Veterinary Science, G. A. D. Veterinary and Animal Sci. Univ., Ludhiana-141 004, India

Abstract

The present study was undertaken to determine the detection threshold of five different Pasteurella multocida PCRs using a standard vaccine strain P52 as DNA template. The purified genomic DNA was diluted ten-fold serially and then subject to polymerase chain reaction. Among the primers assessed, IPFWD or IPREV primers showed the highest sensitivity, detecting purified DNA at 5 fg concentration (~2 bacterial cells). KMT1T7 & KMT1SP6, KTSP61 & KTT72 and PSL-(forward & reverse) primers could detect P52 DNA at the levels of 500, 50 and 50 pg respectively. PM23F1 & PM23R2 primers were the least sensitive and could detect P52 DNA at 5 ng concentration only.

Keywords

Pasteurella multocida Polymerase chain reaction (PCR) Detection threshold.

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