DEEP FREEZING OF BUFFALO SPERMATOZOA IN TRIS AND CITRATE SERIES DILUTORS

Article Id: ARCC3540 | Page : 139-141
Citation :- DEEP FREEZING OF BUFFALO SPERMATOZOA IN TRIS AND CITRATE SERIES DILUTORS.Indian Journal Of Animal Research.2000.(34):139-141
H.P. Guptal and V.B Saxena 1
Address : Govind Ballabh Pant Krishi Evam Praudyogik Vishwavidyalaya, Pantnagar, 263145, India.

Abstract

Initial quality rating of semen had 76.67 ± 4.82% progressively motile, 83.04± 4.97% live and 16.92 ± 3.93% abnormal spermatozoa and 1048. 75 ± 154.79 × 106/ml sperm concentration. The post-thaw sperm motility was 39.58±8.06, 50.42±8.59, 40.93±5.61 and 52.36± 3.63 per cent in egg yolk tris (EYT), EYT + foetal calf serum, egg yolk citrate (EYC) and EYC + foetal calf serum respectively. The values in corresponding dilutors were 54.63 ± 7.26, 65.38 ± 7.73, 51.46 ± 4.64 and 64.27 ± 4.12% for live sperm and 24.92 ± 5.88, 9.2l±2.11, 21.57±3.31 and 12.61±3.84% for abnormal sperm. Highly significant loss in sperm motility and livability was observed at freezing stage in comparison to dilution and equilibration stages in all the dilutors. However, it was low in dilutors supplemented with 10% foetal calf serum. The deep-freezing of buffalo spermatozoa was highly significantly (P< 0.01) better in tris and citrate dilutors supplemented with foetal calf serum in comparison to respective control ones.

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References

  1. Gupta, H.P. and Saxena, V.B. 11996). In : 13th National Convention of ISSAR, Pantnagar.
  2. Gupta, H.P. and Saxena, V.B. (1999). Indian Vet. J. 76: 633-635.
  3. Gupta, H.P et aI., (1984) Indian J Anim. Res., 18.: 81-85.
  4. Kishor, S. (1997). M.V. Sc. Thesis. G.B. Pant Unlv. Agric & Technology, Pantnagar.
  5. Kumar, S. et a/., (1994). Buffalo J 10 : 161-166.
  6. Mauerer, J. P. (1986) In: Animal dell Culture, (Freshney, R.L. ed.).R.L. Press, Oxford.
  7. - Salisbyry, G. W. et a/., (1941). J. 4>airy Sci., 24 : 905-910.
  8. Shabd, R.(1998) M. V.Sc. Thesis. O. B. Pant Univ. of Agric. and Tech. Pantnagar.
  9. Snedecor, G. W. and Cochran, W .G. (1973). Statistical Methods. 6th edn. Oxford and -IBH
  10. Publishing Co., Bombay.
  11. Tomar, N.S. (1984). Artificial Insemirtation and Reproduction of Cattle and Buffaloes. 3rd edn Sa;oj Prakashan, Allahabad.
  12. Vasanth, J. K. (1978) In : Seminar on Buffalo Reproduction and ArtifICIal Insemination. FAO/ SIDA/Animal Reproduction and Health, Paper 13, P- 304-314.
  13. Verma, M.C. et a/., (1975) Indian J. Anim. Sci., 45 : 970-971.

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