volume 34 issue 2 (july to december 2000) : 139-141
DEEP FREEZING OF BUFFALO SPERMATOZOA IN TRIS AND CITRATE SERIES DILUTORS
1Govind Ballabh Pant Krishi Evam Praudyogik Vishwavidyalaya,
Pantnagar, 263145, India.
Published In
Indian Journal of Animal Research
Article Metrics
0
Views
0
Citations
Reviewed By
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
volume 34 issue 2 (july to december 2000) : 139-141
DEEP FREEZING OF BUFFALO SPERMATOZOA IN TRIS AND CITRATE SERIES DILUTORS
1Govind Ballabh Pant Krishi Evam Praudyogik Vishwavidyalaya,
Pantnagar, 263145, India.
ABSTRACT
Initial quality rating of semen had 76.67 ± 4.82% progressively motile, 83.04± 4.97% live and 16.92 ± 3.93% abnormal spermatozoa and 1048. 75 ± 154.79 × 106/ml sperm concentration. The post-thaw sperm motility was 39.58±8.06, 50.42±8.59, 40.93±5.61 and 52.36± 3.63 per cent in egg yolk tris (EYT), EYT + foetal calf serum, egg yolk citrate (EYC) and EYC + foetal calf serum respectively. The values in corresponding dilutors were 54.63 ± 7.26, 65.38 ± 7.73, 51.46 ± 4.64 and 64.27 ± 4.12% for live sperm and 24.92 ± 5.88, 9.2l±2.11, 21.57±3.31 and 12.61±3.84% for abnormal sperm. Highly significant loss in sperm motility and livability was observed at freezing stage in comparison to dilution and equilibration stages in all the dilutors. However, it was low in dilutors supplemented with 10% foetal calf serum. The deep-freezing of buffalo spermatozoa was highly significantly (P< 0.01) better in tris and citrate dilutors supplemented with foetal calf serum in comparison to respective control ones.
KEYWORDS
REFERENCES
- Gupta, H.P. and Saxena, V.B. 11996). In : 13th National Convention of ISSAR, Pantnagar.
- Gupta, H.P. and Saxena, V.B. (1999). Indian Vet. J. 76: 633-635.
- Gupta, H.P et aI., (1984) Indian J Anim. Res., 18.: 81-85.
- Kishor, S. (1997). M.V. Sc. Thesis. G.B. Pant Unlv. Agric & Technology, Pantnagar.
- Kumar, S. et a/., (1994). Buffalo J 10 : 161-166.
- Mauerer, J. P. (1986) In: Animal dell Culture, (Freshney, R.L. ed.).R.L. Press, Oxford.
- - Salisbyry, G. W. et a/., (1941). J. 4>airy Sci., 24 : 905-910.
- Shabd, R.(1998) M. V.Sc. Thesis. O. B. Pant Univ. of Agric. and Tech. Pantnagar.
- Snedecor, G. W. and Cochran, W .G. (1973). Statistical Methods. 6th edn. Oxford and -IBH
- Publishing Co., Bombay.
- Tomar, N.S. (1984). Artificial Insemirtation and Reproduction of Cattle and Buffaloes. 3rd edn Sa;oj Prakashan, Allahabad.
- Vasanth, J. K. (1978) In : Seminar on Buffalo Reproduction and ArtifICIal Insemination. FAO/ SIDA/Animal Reproduction and Health, Paper 13, P- 304-314.
- Verma, M.C. et a/., (1975) Indian J. Anim. Sci., 45 : 970-971.
Disclaimer :
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Copyright :
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
Published In
Indian Journal of Animal Research