Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 34 issue 2 (july to december 2000) : 139-141

DEEP FREEZING OF BUFFALO SPERMATOZOA IN TRIS AND CITRATE SERIES DILUTORS

H.P. Guptal, V.B Saxena 1
1Govind Ballabh Pant Krishi Evam Praudyogik Vishwavidyalaya, Pantnagar, 263145, India.
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Cite article:- Guptal H.P., 1 Saxena V.B (2024). DEEP FREEZING OF BUFFALO SPERMATOZOA IN TRIS AND CITRATE SERIES DILUTORS. Indian Journal of Animal Research. 34(2): 139-141. doi: .
Initial quality rating of semen had 76.67 ± 4.82% progressively motile, 83.04± 4.97% live and 16.92 ± 3.93% abnormal spermatozoa and 1048. 75 ± 154.79 × 106/ml sperm concentration. The post-thaw sperm motility was 39.58±8.06, 50.42±8.59, 40.93±5.61 and 52.36± 3.63 per cent in egg yolk tris (EYT), EYT + foetal calf serum, egg yolk citrate (EYC) and EYC + foetal calf serum respectively. The values in corresponding dilutors were 54.63 ± 7.26, 65.38 ± 7.73, 51.46 ± 4.64 and 64.27 ± 4.12% for live sperm and 24.92 ± 5.88, 9.2l±2.11, 21.57±3.31 and 12.61±3.84% for abnormal sperm. Highly significant loss in sperm motility and livability was observed at freezing stage in comparison to dilution and equilibration stages in all the dilutors. However, it was low in dilutors supplemented with 10% foetal calf serum. The deep-freezing of buffalo spermatozoa was highly significantly (P< 0.01) better in tris and citrate dilutors supplemented with foetal calf serum in comparison to respective control ones.
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