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volume 34 issue 2 (july to december 2000) : 124-126

EFFECT OF SE.RA AND MEDIA ON IN VITRO MATURATION OF - ~'" -., BUBALINE OOCyrES

S
Suresh Kumai'
S
S.N.Mauroya
1Department of Gynaecology and Obstetrics, College Qf Veterinary Sciences G.B. Pant University of Agriculture aI'ld Technology, Pantnagar-263145, India.

  • Submitted|

  • First Online |

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Cite article:- Kumai' Suresh, S.N.Mauroya (2025). EFFECT OF SE.RA AND MEDIA ON IN VITRO MATURATION OF - ~'" -., BUBALINE OOCyrES. Indian Journal of Animal Research. 34(2): 124-126. doi: .

ABSTRACT

Buffalo ovaries were collected from abattoir and oocytes were aspirated from 2–6 mm size follicles. Good quality oocytes were cultured in TCM-199 and HAMS F-10 medium supplemented either with fetal calf serum or bubaline estrus serum or buffalo calf serum. Oocytes were cultured at 38–39°C, with five per cent CO2 and high humidity for 24 hours and then the maturation rate was recorded. The maturation rate observed in TCM-199 containing feotalcalf serum, bubaline estrus serum and buffalo calf serum was 58.87, 67.89 and 53.35 per cent with an overall 60.56 per cent while the corresponding values for Hams F-10 medium were 51.25, 60.83 and 46.35 per cent with an overall average of 53.19 per cent. The present findings conclude that TCM-199 is more suitable for buffalo oocytes than Hams F-10 and the supplementation of bubaline estrus serum can enhance the buffalo oocyte maturation in vitro.

KEYWORDS

    REFERENCES

    1. Motlik, J. and Fulka, J. (1986) Theriogen%gy. 25 : 87- 96.
    2. Reddy, S.M. (1994). Ph.D. Thesis, Bidan Chandra Krishi Vishwa Vidyalaya, Mohanpur, West
    3. Bengal.
    4. Sanbuissho, A and Threfall, W.R. (1990). Theriogen%gy, 35: 341-348.
    5. Se!varaj, R. et a/. (1992) Indian J. Ani. Sci. 62: 434-435.
    6. Snedecor, G.W. and Cochran, W.G. (1998). Statistical Methods. Oxford and IBH Publishing
    7. Co., New Delhi. India.
    8. Totey, S.M. et a/., (1993) Theriogen%gy. 39: 1153-1171.
    9. Totey, S.M. et a/., (1993) J. Repred. FertiJ. 95: 597-607.
    10. Umadevi, S. and Reddy, S.M.(1998) Indian J. Anim Res. 19 : 109-110
    11. Woodlliams, J.A. and Wilmut, T.(1989). Anim. Prod. 48 : 3-30.
    12. Younis, A.1. et a/., (1989) Gamete Res. 23: 189-201.
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    Indian Journal of Animal Research
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      volume 34 issue 2 (july to december 2000) : 124-126

      EFFECT OF SE.RA AND MEDIA ON IN VITRO MATURATION OF - ~'" -., BUBALINE OOCyrES

      S
      Suresh Kumai'
      S
      S.N.Mauroya
      1Department of Gynaecology and Obstetrics, College Qf Veterinary Sciences G.B. Pant University of Agriculture aI'ld Technology, Pantnagar-263145, India.

      • Submitted|

      • First Online |

      • doi

      Cite article:- Kumai' Suresh, S.N.Mauroya (2025). EFFECT OF SE.RA AND MEDIA ON IN VITRO MATURATION OF - ~'" -., BUBALINE OOCyrES. Indian Journal of Animal Research. 34(2): 124-126. doi: .

      ABSTRACT

      Buffalo ovaries were collected from abattoir and oocytes were aspirated from 2–6 mm size follicles. Good quality oocytes were cultured in TCM-199 and HAMS F-10 medium supplemented either with fetal calf serum or bubaline estrus serum or buffalo calf serum. Oocytes were cultured at 38–39°C, with five per cent CO2 and high humidity for 24 hours and then the maturation rate was recorded. The maturation rate observed in TCM-199 containing feotalcalf serum, bubaline estrus serum and buffalo calf serum was 58.87, 67.89 and 53.35 per cent with an overall 60.56 per cent while the corresponding values for Hams F-10 medium were 51.25, 60.83 and 46.35 per cent with an overall average of 53.19 per cent. The present findings conclude that TCM-199 is more suitable for buffalo oocytes than Hams F-10 and the supplementation of bubaline estrus serum can enhance the buffalo oocyte maturation in vitro.

      KEYWORDS

        REFERENCES

        1. Motlik, J. and Fulka, J. (1986) Theriogen%gy. 25 : 87- 96.
        2. Reddy, S.M. (1994). Ph.D. Thesis, Bidan Chandra Krishi Vishwa Vidyalaya, Mohanpur, West
        3. Bengal.
        4. Sanbuissho, A and Threfall, W.R. (1990). Theriogen%gy, 35: 341-348.
        5. Se!varaj, R. et a/. (1992) Indian J. Ani. Sci. 62: 434-435.
        6. Snedecor, G.W. and Cochran, W.G. (1998). Statistical Methods. Oxford and IBH Publishing
        7. Co., New Delhi. India.
        8. Totey, S.M. et a/., (1993) Theriogen%gy. 39: 1153-1171.
        9. Totey, S.M. et a/., (1993) J. Repred. FertiJ. 95: 597-607.
        10. Umadevi, S. and Reddy, S.M.(1998) Indian J. Anim Res. 19 : 109-110
        11. Woodlliams, J.A. and Wilmut, T.(1989). Anim. Prod. 48 : 3-30.
        12. Younis, A.1. et a/., (1989) Gamete Res. 23: 189-201.
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