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Current IssueEditorial BoardOnline First ArticlesArchive

volume 36 issue 1 (january to june 2002) : 17 - 21

BLOOD BIOCHEMICAL ALTERATIONS OF HILL HEIFER CALVES ON FEEDING POLYSTICHUM (POL YSTICHUM SQUARROSUMj FERN*

S
Sanjeev K. Bhure
M
M. Kataria
R
R. Somvanshi
1Indian Veterinary Research Institute, Izatnagar - 243 122, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Bhure K. Sanjeev, Kataria M., Somvanshi R. (2025). BLOOD BIOCHEMICAL ALTERATIONS OF HILL HEIFER CALVES ON FEEDING POLYSTICHUM (POL YSTICHUM SQUARROSUMj FERN*. Indian Journal of Animal Research. 36(1): 17 - 21. doi: .

ABSTRACT

Biochemical alterations in serum, plasma, erythrocytes and polymorphonuclear (PMN) cells of hill heifer calves fed at 30% level of Polystichum squarrosum fern powder for 24 months were studied. Insignificant effect on serum LDH, arginase, and significant decrease in total immunoglobulin, significant increase in plasma fibrinogen and antitrypsin was observed in fern fed calves. Significant decrease in erythrocyte haemoglobin, lipid peroxidation, increase in catalase, reduced glutathione, glutathione-s-transferase and insignificant effect on glutathione reductase was found. Membrane bound enzymes viz. acetyl cholinesterase and different adenosine triphosphatases of erythrocytes were increased in fern fed calves than control. No effect was observed on erythrocytes osmotic fragility, arginase and membrane composition viz. cholesterol, phospholipid, total lipid, total protein and their ratio. Glutathione peroxidase and myeloperoxidase activity in PMN cells were decreased. Results showed that feeding of fern caused suppression of humoral immunity, tissue damage and possibly initiation of tumor formation. The toxic effects were due to effect on oxidant defence, glutathione detoxification system and membrane permeability of erythrocytes.

KEYWORDS

    REFERENCES

    1. Alvin, C.C. eta/. (1999). Proc. Soc. Expt. Biol Moo. 222: 274-282.
    2. Bartlett, G.R. (1959). J. BioL Chern. 234: 466-468.
    3. Bergmeyer, H.U. (1983). In: Methods of Enzymatic Analysis, vol. lII, 3rd edn.. Verlag chemie Weinheim, Deerfield Beach, Florida, Basal, pp 273. .
    4. Bergmeyer, H.U. (1984a). In: Methods of Enzymatic Analysis, Vol. IV, 3rd edn., Verlag chemie Weinheim, Deerfield Beach, Florida, Basal. Pp 57.
    5. Bergmeyer, H.U. (1984b). Arginase. Methods of Enzymatic Analysis. V611Il, 3rd end., Verlag cherriie Weinheim, Deerfield Beach, Florida, Basal, pp. 285.
    6. Carlson, G.P. and Kaneko, J.J. (1973). Proc. Soc. Expt. Biol Moo. 242:853.
    7. Darmstadt, E. Merk. (1974). In: Clinical Laboratory. Medico Chemical Investigation Methods 11th edn. Pp 180..
    8. Dawra, RK. etal (1989). Cancer Lett. 48:143-146.
    9. Folch, J. et al (1957). J. Bioi Chem. 226: 497-509.
    10. Frings, C.S. eta/. (1972). CUn. Chem. 18: 673-674.
    11. Goldberg, D.M. and Spooner, RJ. (1983). In: Methods of Enzymatic Analysis (Bergmeyer, H.U.) Volume 1lI, 3rd edn.
    12. Verlag chemie Weinheim, Deerfield Beach, Florida, Basal. Pp. 258-265.
    13. Habig, WH. etal (1974). J. Biol Chern. 249: 7130-7139.
    14. Manahan, J. and Janice, E. (1974) In: Methods of Enzymology. (Bergmeyer, H.U. ed.) Volume XXXI Part A, Academic Press New York, pp. 168-172.
    15. Jacob, H. and Jandl, H.S. (1966). J. BioL Chern. 241: 4243-4250.
    16. Jain, SK (1988). Biochern. Biophys. Acta. 937:205-210.
    17. Johnson, J.H. and Crinder, B.P. (1989). National Academy of Sciences, USA, 8620,7857.
    18. Kataria, M. and Somvanshi, R (1998). IndianJ. Vet. Res. 7:58-62.
    19. Kataria, M. etal (2000).lndianJ. Toxieo!. 7: 95-101.
    20. Klebenoff,S.J. (1965). EndocrinoL 76: 301-311.
    21. Maida, Y. (1977). Jap. J. Vet. Sci. 39: 187-189.
    22. Mc Evan, A.D. et aI. (1970). Cfin. Chern. Acta. 29: 155-163.
    23. Nanji, A.A. et aI. (1995). J. lipid Res. 36: 736-744.
    24. Oser, B.L. (1971). In: Hawks Physiological Chemistry, 14th edn. Tata Mc Graw Hill. Co. Ltd. New Delhi. Pp. 1083.
    25. Paglia, D.E.and Valentine, WN. (1967). J. Lab. Cfin. Moo. 70: 158-169.
    26. PamuKcu, A.M. and Bryan, G.T. (1976). Arat University Veterinary Fakurt Dergissi. 3: 77.
    27. Parpart, AK et aI. (1947). J. Clin. Invest. 26: 636.
    28. Prince and Loose (1969). Biochemical Method in Red Cell Genetics. Academic Press. Pp 127-129.
    29. Raghuramulu, N. etaI. (1983). In: A Manual of Laboratory Technique. National Institute of Nutrition. Indian Council of Medical Research, Hyderabad. pp. 226.
    30. Richterich, R (1969). Clinical Chemistry. Theory and Practice. S. Karger, Baser. Academic Press. New York. Pp. 336-337.
    31. Snedecor, G.W and Cocharan, WG. (1967). Statistical Methods. 61h edition The Iowa State University Press Ames, Iowa, USA.
    32. Somvanshi, Rand Gounalan, S. (1997). Indian J. Vet. Path. 21: 32-35.
    33. Sunderman, F.W. and Nomoto, S. (1970). C!in. Chern. 16: 903-910.
    34. Taccone, W et a/. (1991). Scandinavian J. cOn. Lab. Invest. 51:25-32.
    35. Vaid, J. et aL (1981). livestock Adviser 6: 55-56.
    36. Yohtalou, T. (1973). Biochern. Biophys. Res. Cornrnun. 55: 630-635.
    37. Yohtalou, T. (1975). Anal Biochem. 63:410-414.
    38. Zlatkis, A. eta/. (1953). J. Lab. Cfin. Moo. 41:486-492.
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      volume 36 issue 1 (january to june 2002) : 17 - 21

      BLOOD BIOCHEMICAL ALTERATIONS OF HILL HEIFER CALVES ON FEEDING POLYSTICHUM (POL YSTICHUM SQUARROSUMj FERN*

      S
      Sanjeev K. Bhure
      M
      M. Kataria
      R
      R. Somvanshi
      1Indian Veterinary Research Institute, Izatnagar - 243 122, India

      • Submitted|

      • First Online |

      • doi

      Cite article:- Bhure K. Sanjeev, Kataria M., Somvanshi R. (2025). BLOOD BIOCHEMICAL ALTERATIONS OF HILL HEIFER CALVES ON FEEDING POLYSTICHUM (POL YSTICHUM SQUARROSUMj FERN*. Indian Journal of Animal Research. 36(1): 17 - 21. doi: .

      ABSTRACT

      Biochemical alterations in serum, plasma, erythrocytes and polymorphonuclear (PMN) cells of hill heifer calves fed at 30% level of Polystichum squarrosum fern powder for 24 months were studied. Insignificant effect on serum LDH, arginase, and significant decrease in total immunoglobulin, significant increase in plasma fibrinogen and antitrypsin was observed in fern fed calves. Significant decrease in erythrocyte haemoglobin, lipid peroxidation, increase in catalase, reduced glutathione, glutathione-s-transferase and insignificant effect on glutathione reductase was found. Membrane bound enzymes viz. acetyl cholinesterase and different adenosine triphosphatases of erythrocytes were increased in fern fed calves than control. No effect was observed on erythrocytes osmotic fragility, arginase and membrane composition viz. cholesterol, phospholipid, total lipid, total protein and their ratio. Glutathione peroxidase and myeloperoxidase activity in PMN cells were decreased. Results showed that feeding of fern caused suppression of humoral immunity, tissue damage and possibly initiation of tumor formation. The toxic effects were due to effect on oxidant defence, glutathione detoxification system and membrane permeability of erythrocytes.

      KEYWORDS

        REFERENCES

        1. Alvin, C.C. eta/. (1999). Proc. Soc. Expt. Biol Moo. 222: 274-282.
        2. Bartlett, G.R. (1959). J. BioL Chern. 234: 466-468.
        3. Bergmeyer, H.U. (1983). In: Methods of Enzymatic Analysis, vol. lII, 3rd edn.. Verlag chemie Weinheim, Deerfield Beach, Florida, Basal, pp 273. .
        4. Bergmeyer, H.U. (1984a). In: Methods of Enzymatic Analysis, Vol. IV, 3rd edn., Verlag chemie Weinheim, Deerfield Beach, Florida, Basal. Pp 57.
        5. Bergmeyer, H.U. (1984b). Arginase. Methods of Enzymatic Analysis. V611Il, 3rd end., Verlag cherriie Weinheim, Deerfield Beach, Florida, Basal, pp. 285.
        6. Carlson, G.P. and Kaneko, J.J. (1973). Proc. Soc. Expt. Biol Moo. 242:853.
        7. Darmstadt, E. Merk. (1974). In: Clinical Laboratory. Medico Chemical Investigation Methods 11th edn. Pp 180..
        8. Dawra, RK. etal (1989). Cancer Lett. 48:143-146.
        9. Folch, J. et al (1957). J. Bioi Chem. 226: 497-509.
        10. Frings, C.S. eta/. (1972). CUn. Chem. 18: 673-674.
        11. Goldberg, D.M. and Spooner, RJ. (1983). In: Methods of Enzymatic Analysis (Bergmeyer, H.U.) Volume 1lI, 3rd edn.
        12. Verlag chemie Weinheim, Deerfield Beach, Florida, Basal. Pp. 258-265.
        13. Habig, WH. etal (1974). J. Biol Chern. 249: 7130-7139.
        14. Manahan, J. and Janice, E. (1974) In: Methods of Enzymology. (Bergmeyer, H.U. ed.) Volume XXXI Part A, Academic Press New York, pp. 168-172.
        15. Jacob, H. and Jandl, H.S. (1966). J. BioL Chern. 241: 4243-4250.
        16. Jain, SK (1988). Biochern. Biophys. Acta. 937:205-210.
        17. Johnson, J.H. and Crinder, B.P. (1989). National Academy of Sciences, USA, 8620,7857.
        18. Kataria, M. and Somvanshi, R (1998). IndianJ. Vet. Res. 7:58-62.
        19. Kataria, M. etal (2000).lndianJ. Toxieo!. 7: 95-101.
        20. Klebenoff,S.J. (1965). EndocrinoL 76: 301-311.
        21. Maida, Y. (1977). Jap. J. Vet. Sci. 39: 187-189.
        22. Mc Evan, A.D. et aI. (1970). Cfin. Chern. Acta. 29: 155-163.
        23. Nanji, A.A. et aI. (1995). J. lipid Res. 36: 736-744.
        24. Oser, B.L. (1971). In: Hawks Physiological Chemistry, 14th edn. Tata Mc Graw Hill. Co. Ltd. New Delhi. Pp. 1083.
        25. Paglia, D.E.and Valentine, WN. (1967). J. Lab. Cfin. Moo. 70: 158-169.
        26. PamuKcu, A.M. and Bryan, G.T. (1976). Arat University Veterinary Fakurt Dergissi. 3: 77.
        27. Parpart, AK et aI. (1947). J. Clin. Invest. 26: 636.
        28. Prince and Loose (1969). Biochemical Method in Red Cell Genetics. Academic Press. Pp 127-129.
        29. Raghuramulu, N. etaI. (1983). In: A Manual of Laboratory Technique. National Institute of Nutrition. Indian Council of Medical Research, Hyderabad. pp. 226.
        30. Richterich, R (1969). Clinical Chemistry. Theory and Practice. S. Karger, Baser. Academic Press. New York. Pp. 336-337.
        31. Snedecor, G.W and Cocharan, WG. (1967). Statistical Methods. 61h edition The Iowa State University Press Ames, Iowa, USA.
        32. Somvanshi, Rand Gounalan, S. (1997). Indian J. Vet. Path. 21: 32-35.
        33. Sunderman, F.W. and Nomoto, S. (1970). C!in. Chern. 16: 903-910.
        34. Taccone, W et a/. (1991). Scandinavian J. cOn. Lab. Invest. 51:25-32.
        35. Vaid, J. et aL (1981). livestock Adviser 6: 55-56.
        36. Yohtalou, T. (1973). Biochern. Biophys. Res. Cornrnun. 55: 630-635.
        37. Yohtalou, T. (1975). Anal Biochem. 63:410-414.
        38. Zlatkis, A. eta/. (1953). J. Lab. Cfin. Moo. 41:486-492.
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