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M. R. Saseendranath

Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA

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USE OF CYSTIC FOLUCUI.AR FLUID FOR BUFFALO OOCYTE MATURATION IN VITRO

S. Nandi, P.S.P. Gupta, B.M. Ravindranatha, P.V. Sarma

Affiliations

¹National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore - 560 030, India

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Cite article:- Nandi S., Gupta P.S.P., Ravindranatha B.M., Sarma P.V. (2025). USE OF CYSTIC FOLUCUI.AR FLUID FOR BUFFALO OOCYTE MATURATION IN VITRO. Indian Journal of Animal Research. 37(1): 40 - 43. doi: .

ABSTRACT

Studies were conducted to examine the effect of substitution of serum with cystic follicular fluid in
the IVM media and also to test the efficacy of cystic follicular fluid (buCFF) at 50 and 100 per cent
levels for in vitro maturation of buffalo oocytes. Resutts indicated that buffalo cystic follicular fluid
supplemented with PMSG possess the ability for induction of maturation of buffalo oocytes and could be successfully tried as buffalo oocyte maturation medium. Buffalo cystic follicular fluid as a whole naturation medium· also has the ability to induce the cumulus expansion which may be cost effective for the IVM of buffalo oocytes.

KEYWORDS

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