Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 38 issue 2 (july to december 2004) : 112 - 116


A.K. Pandey, H.P. Gupta
1Department of Animal Reproduction, Gynaecology and Obstetrics, CoUelge of Veterinary and Animal Sciences, Pantnagar - 263 145, India
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Cite article:- Pandey A.K., Gupta H.P. (2024). EVALUATION OFVARIOUS DILUTORS BY ACROSOME REACTION TESr. Indian Journal of Animal Research. 38(2): 112 - 116. doi: .
An experiment was conducted to study the freezability of unwashed and washed buffalo spermatozoa in egg yolk tris (EYT) dilutor modified by addition of 0, 3, 6 and 10% fetal calf serum (EYT-So, EYT-S1, EYT-S2 and EYT-S3, respectively). Acrosome reaction test, in addition to sperm motility, livability and total sperm abnormalities, was performed at 0 hr (after dilution), PF (pre-freezing/after equilibration) and PT (post thaw) stages to evaluate the dilutors for maximum recovery of post-thaw fertilizing ability, motility and livability. Washing significantly (P < 0.01) affected acrosome reaction percentage, sperm motility, livability and total abnormalities. Dilutors were non-significant on all parameters, whereas acrosome reacting spermatozoa were higher in EYT-S3 dilutor for unwahed and in EYT-S2 dilutor for washed samples. PT motility and livability were higher in EYT-S2 dilutor as compared to control for both unwashed and washed samples. Total sperm abnormalities were within normallirnits in all dilutors at all stages.
    1. Amman, R.P. (1989). J. Andrology, 10: 89-98.
    2. Bavister, B.D. and Yanagimachi, R. (1977). BioI. Reprod., 16: 228-237.
    3. Brown, O.v. and Senger, P.L. (1980). BioI. Reprod., 23: 271-275.
    4. Correa, J.R. etal. (1997). Theriogenology, 48: 721-731.
    5. Gupta, H.P. and Saxena, V.B. (1996). 13th National Convention of Indian Society for the Study of Animal Reproduction and Nationai ,symposium on Animal Biotechnology. G.B.P.U.A and T., Pantnagar (India).
    6. Gupta, H.P. and Saxena, V.B. (1999). Indian Vet. J., 76: 633-635.
    7. Hazarika, H. (1986). M.v.Sc. Thesis. G B Pant University of Agric. and Techno!., Pantnagar (India).
    8. Kishor, S. (1997). M.v.Sc. Thesis. G B Pant University of Agric. and Techno!., Pantnagar (India).
    9. Kumar, S. (2000). Ph.D. Thesis. G B Pant U"iversity of Agric. and Techno!., Pantnagar (India).
    10. Mauerer, J.P. (1986). In: Animal Cell Culture (Freshney, R.L. Eel.). IRL Press, Oxford.
    11. Parrish, J.J. etal. (1985). BioI. Reprod., 32(Suppl. 1): 211.
    12. Revell, S.G. et al. (1997). Proc. Joint Meeting of the Society for the Study of Fertility. British Fertility Society and
    13. British Andrology Society, Abstract Series No. 19, July 1997, Abstract No. 92, p. 38.
    14. Senger, P.L. and Saacke, R.G. (1976). J. Reprod. FertiJ., 47: 215-219.
    15. Sengupta, B.P. et al. (1976). Curro Sci., 45: 2-8.
    16. Shabd, R. (1998). M.v.Sc. Thesis. G B Pant University of Agric. and Technol., Pantnagar, (India).
    17. Snedecor, G.W. and Cochran, WG. (1967). Statistical Methods. 6th Edn. Oxford and IBH Publishing Co., New Delhi.
    18. Tomar, N.S. (1997). Artificial Insemination and Reproduction of Cattle and Buffaloes. 4th Edn. Saroj Prakashan, Allahabad.
    19. Vasanth, J.K. (1978). Seminar on Buffalo Reproduction and Artificial Insemination. FAO/SIDNAnimai Reproduction and Health, 13: 304-314.
    20. Verma, M.C. eta/. (1975).lndianJ. Anim. Se1., 45: 970-971.
    21. Whitfield, C.H. and Parkinson, T.J. (1992). Theriogenology, 38: 11-20.
    22. Whitfield, C.H. and Parkinson T.J. (1995). Theriogenology, 44: 413-422

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