Indian Journal of Animal Research, volume 38 issue 2 (july to december 2004) : 112 - 116

EVALUATION OFVARIOUS DILUTORS BY ACROSOME REACTION TESr

A.K. Pandey, H.P. Gupta

Affiliations

¹Department of Animal Reproduction, Gynaecology and Obstetrics, CoUelge of Veterinary and Animal Sciences, Pantnagar - 263 145, India

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Cite article:- Pandey A.K., Gupta H.P. (2025). EVALUATION OFVARIOUS DILUTORS BY ACROSOME REACTION TESr. Indian Journal of Animal Research. 38(2): 112 - 116. doi: .

ABSTRACT

An experiment was conducted to study the freezability of unwashed and washed buffalo spermatozoa in egg yolk tris (EYT) dilutor modified by addition of 0, 3, 6 and 10% fetal calf serum (EYT-So, EYT-S1, EYT-S2 and EYT-S3, respectively). Acrosome reaction test, in addition to sperm motility, livability and total sperm abnormalities, was performed at 0 hr (after dilution), PF (pre-freezing/after equilibration) and PT (post thaw) stages to evaluate the dilutors for maximum recovery of post-thaw fertilizing ability, motility and livability. Washing significantly (P < 0.01) affected acrosome reaction percentage, sperm motility, livability and total abnormalities. Dilutors were non-significant on all parameters, whereas acrosome reacting spermatozoa were higher in EYT-S3 dilutor for unwahed and in EYT-S2 dilutor for washed samples. PT motility and livability were higher in EYT-S2 dilutor as compared to control for both unwashed and washed samples. Total sperm abnormalities were within normallirnits in all dilutors at all stages.

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EVALUATION OFVARIOUS DILUTORS BY ACROSOME REACTION TESr

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