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KINETICS OF ALKALINE PHOSPHATASE ISOLATED FROM RAT LIVER, KIDNEY AND CERTAIN BRAIN REGIONS

N.H. Mohan1, S.K. Maurya, A.K Gangwar, R.S. Srivastava2

Affiliations

¹Department of Veterinary Physiology, College of Veterinary Science and A.H., NDUAT, Kumarganj, Faizabad (UP) - 224 229, India

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Cite article:- Mohan1 N.H., Maurya S.K., Gangwar A.K, Srivastava2 R.S. (2025). KINETICS OF ALKALINE PHOSPHATASE ISOLATED FROM RAT LIVER, KIDNEY AND CERTAIN BRAIN REGIONS. Indian Journal of Animal Research. 39(2): 123 - 126. doi: .

ABSTRACT

In the present study, the kinetics of alkaline phosphatase (ALP) isoforms partially purified
from rat liver, kidney, hypothalamus and cerebro-cortical region of brain is reported. The MichaelisMenten
constant (Km,micromoles) of the enzyme for the substrate p-nitrophenyl phosphate
were 2.67,2.50 2.85 and 3.33 for the enzymes-obtainecJ-from liver, kidney, hypothalamus and
cortical region respectively. The corresponding V..... values (nanomoles/milligram protein/minute)
were 1.03, 1.43, 1.33 and 2.22 respectively. The fraction of catalytic sites filled in the enzyme
by the substrate was also calculated. For any substrate concentration, the fraction of catalytic
sites filled was highest for renal ALP and lowest for cortical one. The variation in the properties
of these tissue specific or unspecific enzymes may be due to differential expression of ALP
genes or post-translational modifications. Since there was no significant difference between
these values it was concluded that the kinetics of ALP for p-nitrophenyl phosphate substrate
alone is not sufficient to distinguish these clinically important isoforms

KEYWORDS

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