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Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA

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DETERMINATION OF GESTATION PERIOD OF MARE WITH MAXIMUM eCG CONCENTRATION BY BIOASSAY

Meenakshi Virmani, A.K. Gupta*, S.K. Garg

Affiliations

¹Department of Veterinary Physiology, College of Veterinary Sciences, CCSHAU, Hisar - 125 004, India

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Cite article:- Virmani Meenakshi, Gupta* A.K., Garg S.K. (2025). DETERMINATION OF GESTATION PERIOD OF MARE WITH MAXIMUM eCG CONCENTRATION BY BIOASSAY. Indian Journal of Animal Research. 40(1): 25 - 29. doi: .

ABSTRACT

The concentration of eCG in pregnant mare serum at different days of gestation has been
determined by bioassay (A-Z test). The studies revealed that 0.2 ml volume of pregnant mare
serum (PMS) is good enough for inoculation in immature mice to produce sexual maturity. It has
also been depicted that the concentration of equine chorionic gonadotrophin (eCG) in serum of
pregnant mares is nil till 30th day of gestation. The eCG level rises in serum by 45th day of
gestation, reaches a peak of 62.5 IU/ml serum by 90th day and declines thereafter to a
concentration of 15.0 IU/ml by 135th day of gestation. The eCG activity is maximum during
60-90 days of gestation in PMS.

KEYWORDS

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