Diagnosis of Mycobacterium bovis is important from the public health perspective since control
measures, treatment are very significant, particularly in wild animals. To diagnose M. bovis infection in wild animals polymerase chain reaction had been used, using the primer sequence of pnc A 8 and pncA 11 that are specific for M. bovis pnc A gene. A 744 bp PCR product was generated out of 3 samples that consisted of blood, caseated nodule, and lung tissues collected from hyena, bear and lion maintained at Arignar Anna Zoological Park, Chennai, Tamil Nadu and were identified as M. bovis. A positive control of M. bovis DNA procured from Tuberculosis Research Centre (TRC), Chennai, Tamil Nadu was used and a negative control of a healthy bovine blood was used. The present study demonstrates the use of PCR as a sensitive diagnostic gadget for diagnosing bovine tuberculosis (BTB) among wild animals.