Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 42 issue 4 (december 2008) : 242-247

SPERM MEMBRANE/SEMINAL PLASMA PROTEINS REFLECTS SEMEN QUALITY IN CROSS-BRED CATTLE BULLS

S. Ranjna Cheema, B.K. Babbar*
1Department of Animal Breeding and Genetics, Guru Angad Dev Veternary and Animal Science University, Ludhiana-141 004, India

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Cite article:- Cheema Ranjna S., Babbar* B.K. (2024). SPERM MEMBRANE/SEMINAL PLASMA PROTEINS REFLECTS SEMEN QUALITY IN CROSS-BRED CATTLE BULLS. Indian Journal of Animal Research. 42(4): 242-247. doi: .

ABSTRACT

The sperm membrane proteins (SMP) and seminal plasma proteins (SPP) were analyzed and
characterized in freshly ejaculated semen of 14 crossbred cattle bulls. The protein content and
profile were correlated to percent swollen spermatozoa in Hypo-osmotic swelling (HOS) buffer and
freezability of semen. Higher amount of extraction of sperm membrane proteins and low rate of
freezability and HOS positive spermatozoa in bull # 5,6,12 and 13 indicated low sperm membrane
integrity in these bulls. The presence of 35 kDa and 220 kDa proteins in the SME of bulls with higher
rate of semen freezability and HOS positive spermatozoa can be correlated to the good quality/
fertility of semen. The presence of >240 kDa, 130 kDa and 220 kDa poteins in SP of bulls with
lower-, higher- and non freezability of semen respectively can also be correlated to the rate of
fertility of bulls.

KEYWORDS

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