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Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Indian Journal of Animal Research, volume 47 issue 6 (december 2013) : 472-478

DETECTION AND MOLECULAR CLONING OF GROWTH DIFFERENTIATION FACTOR-9 TRANSCRIPTS IN IN VITRO MATURED BUFFALO OOCYTE COMPLEXES

H.N. Malik*, S.K. Dash1, S.K. Mohapatra2, T. Nanda
1Animal Biotechnology Center, CCS Haryana Agricultrual University, Hisar-125 004, India

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Cite article:- Malik* H.N., Dash1 S.K., Mohapatra2 S.K., Nanda T. (2025). DETECTION AND MOLECULAR CLONING OF GROWTH DIFFERENTIATION FACTOR-9 TRANSCRIPTS IN IN VITRO MATURED BUFFALO OOCYTE COMPLEXES. Indian Journal of Animal Research. 47(6): 472-478. doi: .

ABSTRACT

The present study was carried out on buffalo ovarian follicles. From these follicles cumulus oocyte complexes were aspirated and in vitro matured in TCM-199 supplemented 10% v/v fetal bovine serum, 1µg/ml estradiol, 10µg/ml LH, 5µg/ml FSH and 1% v/v gentamycin. In vitro matured cumulus oocyte complexes were investigated for the presence of GDF -9 gene transcript using RT-PCR analysis. After the RT-PCR analysis the amplified product was purified and cloned. The cloned product was further sequenced and analyzed to study the phylogenetic relationship of GDF-9 gene of buffalo with other species. Upon RT-PCR analysis it was revealed that GDF-9 transcripts were expressed exclusively in in vitro matured cumulus oocyte complexes. Nucleotide sequence analysis of GDF-9 gene of Bubalus bubalis revealed that it is 99% similar to that of Bos taurus and clustered together with Bos taurus and Rattus norvegicus shared a separate cluster.

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