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Current IssueEditorial BoardOnline First ArticlesArchive

volume 47 issue 6 (december 2013) : 472-478

DETECTION AND MOLECULAR CLONING OF GROWTH DIFFERENTIATION FACTOR-9 TRANSCRIPTS IN IN VITRO MATURED BUFFALO OOCYTE COMPLEXES

H
H.N. Malik*
S
S.K. Dash1
S
S.K. Mohapatra2
T
T. Nanda
1Animal Biotechnology Center, CCS Haryana Agricultrual University, Hisar-125 004, India

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Cite article:- Malik* H.N., Dash1 S.K., Mohapatra2 S.K., Nanda T. (2025). DETECTION AND MOLECULAR CLONING OF GROWTH DIFFERENTIATION FACTOR-9 TRANSCRIPTS IN IN VITRO MATURED BUFFALO OOCYTE COMPLEXES. Indian Journal of Animal Research. 47(6): 472-478. doi: .

ABSTRACT

The present study was carried out on buffalo ovarian follicles. From these follicles cumulus oocyte complexes were aspirated and in vitro matured in TCM-199 supplemented 10% v/v fetal bovine serum, 1µg/ml estradiol, 10µg/ml LH, 5µg/ml FSH and 1% v/v gentamycin. In vitro matured cumulus oocyte complexes were investigated for the presence of GDF -9 gene transcript using RT-PCR analysis. After the RT-PCR analysis the amplified product was purified and cloned. The cloned product was further sequenced and analyzed to study the phylogenetic relationship of GDF-9 gene of buffalo with other species. Upon RT-PCR analysis it was revealed that GDF-9 transcripts were expressed exclusively in in vitro matured cumulus oocyte complexes. Nucleotide sequence analysis of GDF-9 gene of Bubalus bubalis revealed that it is 99% similar to that of Bos taurus and clustered together with Bos taurus and Rattus norvegicus shared a separate cluster.

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Indian Journal of Animal Research
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