Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 47 issue 5 (october 2013) : 456-459


K.P. Shyam, S.K. Gupta, Ajit Singh, S.S. Chaudhary, Jay Prakash Gupta
1Department of Veterinary Parasitology and Microbiology College of Veterinary Sciences, LLR University of Veterinary & Animal Sciences, Hisar- 125 004, India
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Cite article:- Shyam K.P., Gupta S.K., Singh Ajit, Chaudhary S.S., Gupta Prakash Jay (2023). DETECTION OF TRYPANOSOMA EVANSI IN WHOLE BLOOD OF DOMESTIC ANIMALS BY DNA AMPLIFICATION METHOD. Indian Journal of Animal Research. 47(5): 456-459. doi: .
Trypanosoma evansi, a blood protozoan parasite causing ‘surra’ in domestic animals is widely prevalent in different parts and is of significant economic importance in livestock production. Parasitaemia is often intermittent and is not always possible to demonstrate the parasites in blood and hence diagnosis of trypanosomosis is often difficult. The present investigation has been carried out with the aim of detecting T. evansi in cattle, buffaloes and equines in the state of Haryana by parasitological (WBF), and DNA-detecting (TE-PCR) tests. Out of 205 field blood samples tested, only 2% were positive for T. evansi by WBF while PCR using synthetic oligonulceotide primers (21 mer sense and 22 mer antisense) targeted to a repetitive nuclear DNA sequence of T. evansi detected 60.49% samples positive. Study revealed TE-PCR to be highly sensitive to the conventional parasitological method and could be used for early of diagnosis of trypanosomosis in domestic animals.
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