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volume 47 issue 4 (august 2013) : 360-363

DGAT1 POLYMORPHISM K232A IN SAHIWAL (INDIAN ZEBU) AND FRIESWAL (HOLSTEIN FRIESIAN X SAHIWAL CROSSBRED) CATTLE

I
Indrajit Ganguly1*
S
Sushil Kumar
G
G. K. Gaur1
U
Umesh Singh
A
Arun Kumar2
S
Sunil Kumar
S
Sandeep Mann
1Molecular Genetics Laboratory, Project Directorate on Cattle, Meerut Cantt-250 001, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Ganguly1* Indrajit, Kumar Sushil, Gaur1 K. G., Singh Umesh, Kumar2 Arun, Kumar Sunil, Mann Sandeep (2025). DGAT1 POLYMORPHISM K232A IN SAHIWAL (INDIAN ZEBU) AND FRIESWAL (HOLSTEIN FRIESIAN X SAHIWAL CROSSBRED) CATTLE. Indian Journal of Animal Research. 47(4): 360-363. doi: .

ABSTRACT

The positional candidate gene DGAT1, within QTL of BTA14, harboring a lysine to alanine substitution (K232A) is significantly associated with milk production traits. The present investigation was undertaken to estimate the frequency of the DGAT1 K232A polymorphism in Indian zebu breed, Sahiwal (B. indicus; N=51) and crossbred, Frieswal (HF X Sahiwal; N=126) cattle using PCR-RFLP technique. Detection of allelic variation at nucleotide positions 10433–10434 of the DGAT1 gene was performed by restriction digestion of a 413 bp PCR product with CfrI. The frequency of A allele in Frieswal and Sahiwal cattle was 0.36 and 0.04, respectively. Although all the three genotypes were observed in Frieswal cattle, however, no animal of AA genotype was found in Sahiwal cattle and only four animals were of heterozygous genotype (AK). The sequences, specific to K and A allele, were submitted to GenBank (JF345253 and JF345254). Because of very high frequency to almost fixed nature of K allele in Sahiwal and other well defined Indian cattle breeds, DGAT1 K232A polymorphism may not be a suitable candidate for selection purpose. Nonetheless, the results could be used to guide association studies between this locus and milk production traits especially in Frieswal.

REFERENCES

  1. Cases S, Smith SJ, Zheng YW, Myers HM, Lear SR, Sande E,Novak S, Collins C, Welch CB, Lusis AJ, et al. (1998) Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc. Natl. Acad.Sci. USA., 95:13018-13023.
  2. Coppieters, W., Riquet, J., Arranz, J. J., Berzi, P., Cambisano, N., Grisart, B., Karim, L., Marcq, F., Moreau, L., Nezer, C., Simon, P., Vanmanshoven, P., Wagenaar, D. and Georges, M. (1998). A QTL with major effect on milk yield and composition maps to bovine chromosome 14. Mamm. Genome, 9:540-544.
  3. Grisart, B., Coppieters, W, Farnir, F., Karim, L., Ford, C., Berzi, P., Cambisano, N., Mni, M., Reid, S., Simon, P., Spelman, R., Georges, M. and Snell, R. (2002). Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition Genome Res., 12:222-231.
  4. Kaupe, B., Brandt, H., Prinzenberg, E.M. and Erhardt, G. (2007). Joint analysis of the influence of CYP11B1 and DGAT1 genetic variation on milk production, somatic cell score, conformation, reproduction, and productive lifespan in German Holstein cattle. J. Anim. Sci., 85: 11-21.
  5. Kaupe, B., Winter, A., Fries, R. and Erhardt, G. (2004). DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds. Anim. Genet., 71:182-187.
  6. Nowacka-Woszuk, J., Noskowiak, A., Strabel, T., Jankowski, T. and Œwitoñski, M. (2008). An effect of the DGAT1 gene polymorphism on breeding value of Polish Holstein-Friesian sires. Anim. Sci. Paper Report, Institute of Genetics and Animal Breeding, Jastrzêbiec, Poland 26:17-23.
  7. Schennink, A., Stoop, W.M., Visker, M.H., Heck, J.M., Bovenhuis, H., van der Poel, j.j., van Valenberg, H.j. and van Arendonk J.A. (2007). DGAT1 underlies large genetic variation in milk-fat composition of dairy cows. Anim. Genet., 38: 467-473.
  8. Szyda, J. and Komisarek, J. (2007). Statistical modeling of candidate gene effects on milk production traits in dairy cattle. J. Dairy Sci., 90:2971-2979.
  9. Tantia, M.S., Vijh, R.K., Mishra, B.P., Mishra, B., Kumar, S.T.B. and Sodhi, M. (2006). DGAT1 and ABCG2 polymorphism in Indian cattle (Bos indicus) and buffalo (Bubalus bubalis) breeds. BMC Vet. Res., 2:32.
  10. Winter, A., Krämer, W., Werner, F.A.O., Kollers, S., Kata, S., Durstewitz, G., Buitkamp, J., Womack, J.E., Thaller, G. and Fries, R. (2002). Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl- CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proc. Natl. Acad. Sci. USA., 99:9300-9305.
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Indian Journal of Animal Research
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    volume 47 issue 4 (august 2013) : 360-363

    DGAT1 POLYMORPHISM K232A IN SAHIWAL (INDIAN ZEBU) AND FRIESWAL (HOLSTEIN FRIESIAN X SAHIWAL CROSSBRED) CATTLE

    I
    Indrajit Ganguly1*
    S
    Sushil Kumar
    G
    G. K. Gaur1
    U
    Umesh Singh
    A
    Arun Kumar2
    S
    Sunil Kumar
    S
    Sandeep Mann
    1Molecular Genetics Laboratory, Project Directorate on Cattle, Meerut Cantt-250 001, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Ganguly1* Indrajit, Kumar Sushil, Gaur1 K. G., Singh Umesh, Kumar2 Arun, Kumar Sunil, Mann Sandeep (2025). DGAT1 POLYMORPHISM K232A IN SAHIWAL (INDIAN ZEBU) AND FRIESWAL (HOLSTEIN FRIESIAN X SAHIWAL CROSSBRED) CATTLE. Indian Journal of Animal Research. 47(4): 360-363. doi: .

    ABSTRACT

    The positional candidate gene DGAT1, within QTL of BTA14, harboring a lysine to alanine substitution (K232A) is significantly associated with milk production traits. The present investigation was undertaken to estimate the frequency of the DGAT1 K232A polymorphism in Indian zebu breed, Sahiwal (B. indicus; N=51) and crossbred, Frieswal (HF X Sahiwal; N=126) cattle using PCR-RFLP technique. Detection of allelic variation at nucleotide positions 10433–10434 of the DGAT1 gene was performed by restriction digestion of a 413 bp PCR product with CfrI. The frequency of A allele in Frieswal and Sahiwal cattle was 0.36 and 0.04, respectively. Although all the three genotypes were observed in Frieswal cattle, however, no animal of AA genotype was found in Sahiwal cattle and only four animals were of heterozygous genotype (AK). The sequences, specific to K and A allele, were submitted to GenBank (JF345253 and JF345254). Because of very high frequency to almost fixed nature of K allele in Sahiwal and other well defined Indian cattle breeds, DGAT1 K232A polymorphism may not be a suitable candidate for selection purpose. Nonetheless, the results could be used to guide association studies between this locus and milk production traits especially in Frieswal.

    REFERENCES

    1. Cases S, Smith SJ, Zheng YW, Myers HM, Lear SR, Sande E,Novak S, Collins C, Welch CB, Lusis AJ, et al. (1998) Identification of a gene encoding an acyl CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis. Proc. Natl. Acad.Sci. USA., 95:13018-13023.
    2. Coppieters, W., Riquet, J., Arranz, J. J., Berzi, P., Cambisano, N., Grisart, B., Karim, L., Marcq, F., Moreau, L., Nezer, C., Simon, P., Vanmanshoven, P., Wagenaar, D. and Georges, M. (1998). A QTL with major effect on milk yield and composition maps to bovine chromosome 14. Mamm. Genome, 9:540-544.
    3. Grisart, B., Coppieters, W, Farnir, F., Karim, L., Ford, C., Berzi, P., Cambisano, N., Mni, M., Reid, S., Simon, P., Spelman, R., Georges, M. and Snell, R. (2002). Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition Genome Res., 12:222-231.
    4. Kaupe, B., Brandt, H., Prinzenberg, E.M. and Erhardt, G. (2007). Joint analysis of the influence of CYP11B1 and DGAT1 genetic variation on milk production, somatic cell score, conformation, reproduction, and productive lifespan in German Holstein cattle. J. Anim. Sci., 85: 11-21.
    5. Kaupe, B., Winter, A., Fries, R. and Erhardt, G. (2004). DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds. Anim. Genet., 71:182-187.
    6. Nowacka-Woszuk, J., Noskowiak, A., Strabel, T., Jankowski, T. and Œwitoñski, M. (2008). An effect of the DGAT1 gene polymorphism on breeding value of Polish Holstein-Friesian sires. Anim. Sci. Paper Report, Institute of Genetics and Animal Breeding, Jastrzêbiec, Poland 26:17-23.
    7. Schennink, A., Stoop, W.M., Visker, M.H., Heck, J.M., Bovenhuis, H., van der Poel, j.j., van Valenberg, H.j. and van Arendonk J.A. (2007). DGAT1 underlies large genetic variation in milk-fat composition of dairy cows. Anim. Genet., 38: 467-473.
    8. Szyda, J. and Komisarek, J. (2007). Statistical modeling of candidate gene effects on milk production traits in dairy cattle. J. Dairy Sci., 90:2971-2979.
    9. Tantia, M.S., Vijh, R.K., Mishra, B.P., Mishra, B., Kumar, S.T.B. and Sodhi, M. (2006). DGAT1 and ABCG2 polymorphism in Indian cattle (Bos indicus) and buffalo (Bubalus bubalis) breeds. BMC Vet. Res., 2:32.
    10. Winter, A., Krämer, W., Werner, F.A.O., Kollers, S., Kata, S., Durstewitz, G., Buitkamp, J., Womack, J.E., Thaller, G. and Fries, R. (2002). Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl- CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proc. Natl. Acad. Sci. USA., 99:9300-9305.
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