Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

volume 47 issue 4 (august 2013) : 331-334

LYMPHOCYTIC NITRIC OXIDE SECRETION DURING DIFFERENT REPRODUCTIVE PHASES IN CATTLE

M
M.K. Rose
1Department of Veterinary Physiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125 004, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Rose M.K. (2025). LYMPHOCYTIC NITRIC OXIDE SECRETION DURING DIFFERENT REPRODUCTIVE PHASES IN CATTLE. Indian Journal of Animal Research. 47(4): 331-334. doi: .

ABSTRACT

Nitric oxide (NO) is a biological mediator involved in regulation of various physiological processes including reproduction. NO is produced by many different cells of the body including lymphocytes. In this study, the lymphocytic NO secretion was studied in cattle during different phases of reproduction. Blood samples were collected from Holstein Friesian cows during different stages of estrous cycle like follicular phase and luteal phase and also during various stages of pregnancy like early-pregnancy (d 61-83), mid-pregnancy (d 100-168) and late-pregnancy (d 239-246). Six animals at age of 3-7 years were used in each group. Lymphocytes were separated by density gradient centrifugation technique, and cultured for 72 hours. Then culture supernatant was used for measurement of NO by colourimetric assay based on the Griess reaction. Plasma levels of estradiol and progesterone were also measured by enzyme-immuno assay (EIA) to ascertain the stage of estrous cycle. Lymphocytic NO secretion during mid pregnancy (d 100-168) (35.36±1.18 µM nitrite) was significantly higher than follicular phase (26.60±3.71 µM nitrite, P

REFERENCES

  1. Ali, M.; Buhimschi, I.; Chwalisz, K. and Garfield, R.E. (1997). Changes in expression of the nitric oxide synthease isoforms in rat uteus and cervix during pregnancy and parturition. Mol. Hum. Reprod. 3: 995-1003.
  2. Bellefontaine, N.; Hanchate, N.K.; Parkash, J.; Campagne, C.; de Seranno, S.; Clasadonte, J.; d’Anglemont de Tassigny, X. and Prevot, V. (2011) Nitric oxide as key mediator of neuron-to-neuron and endothelia-to-glia communication involved in the neuroendocrine control of reproduction. Neuroendocrinol. 93 (2): 74-89.
  3. Biswas, S.; Kabir, S.N. and Pal, A.K. (1998) The role of nitric oxide in the process of implantation in rats. J. Reprod. Fert. 114: 157-161.
  4. Bredt, D.S. and Snyder, S.H. (1994) Nitric oxide: A physiologic messenger molecule. Annu. Rev. Biochem. 63: 175-195.
  5. Dixit, V. D. and Parvizi, N. (2001) Pregnancy stimulates secretion of adrenocroticotropin and nitric oxide from peripheral bovine lymphocytes. Bol. Reprod. 64: 242-248.
  6. Dixit, V. D. and Parvizi, N. (2001b) Nitric oxide and the control of reproduction. Anim. Reprod. Sci. 65: 1-16.
  7. Glatz-Vinke, B. (1998) Lymphocytic hormone secretion: secretion and regulation of ACTH in pigs and GH and ACTH in cattle. Ph.D. Thesis, Vet. Med. School, Hannover, Germany.
  8. Goerner, C. (1995) Untersuchungen zur immuneendokrinen Intraction bein Schwein. Wachstumshormonsekretion bei Immunzellen. Ph.D. Thesis, Vet. Med. School, Hannover, Germany.
  9. Lee, S.H.; Acosta, T.J.; Yoshioka, S. and Okuda, K. (2009) Prostaglandin F2 alpha regulates the nitric oxide generating system in bovine luteal endothelial cells. J. Reprod. Dev. 55:418-424.
  10. Luo, Q.; Chen, X.J.; Ding, G.L.; Dong, M.Y. and Huang, H.F. (2010) Downregulative effects of nitric oxide on oocyte fertilization and embryo development: possible roles of nitric oxide in the pathogenesis of endometriosis-associated infertility. Cell Physiol. Biochem. 26 : 1023-1028.
  11. Motran, C.C.; Diaz, F. L.; Gruppi, A.; Slavin, D.; Chatton, B. and Bocco, J.L. (2002) Human pregnancy specific glycoprotein 1a (PSG1a) induces alternative activation in human and mouse monocytes and suppresses the accessory cell-dependent T cell proliferation. J. Leukoc. Biol. 72 : 512-521.
  12. Pancarci, S.M.; Gungor, O.; Atakisi, O.; Cigremis, Y.; Ari, U.C. and Bollwein H. (2011) Changes in follicular blood flow and nitric oxide levels in follicular fluid during follicular deviation in cows. Anim. Reprod. Sci. 123 : 149-156.
  13. Shukovski, L.; and Tsafriri, A. (1994) The involvement of nitric oxide in the ovulatory process in the rat. Endocrinol. 135: 2287-2290.
  14. Sirotkin, A.V. and Grossmann, R. (2003) Role of tyrosine kinase- and MAP kinase-dependent intracellular mechanisms in control of ovarian functions in the domestic fowl (Gallus domesticus) and in mediating effects of IGF-II. J. Reprod. Dev., 49: 99-106.
  15. Sladek, S.M. and Magness, R.R. and Conrad, K.P. (1997) Nitric oxide and pregnancy. Am. J. Physiol. 272 (2): R441-R463.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    volume 47 issue 4 (august 2013) : 331-334

    LYMPHOCYTIC NITRIC OXIDE SECRETION DURING DIFFERENT REPRODUCTIVE PHASES IN CATTLE

    M
    M.K. Rose
    1Department of Veterinary Physiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125 004, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Rose M.K. (2025). LYMPHOCYTIC NITRIC OXIDE SECRETION DURING DIFFERENT REPRODUCTIVE PHASES IN CATTLE. Indian Journal of Animal Research. 47(4): 331-334. doi: .

    ABSTRACT

    Nitric oxide (NO) is a biological mediator involved in regulation of various physiological processes including reproduction. NO is produced by many different cells of the body including lymphocytes. In this study, the lymphocytic NO secretion was studied in cattle during different phases of reproduction. Blood samples were collected from Holstein Friesian cows during different stages of estrous cycle like follicular phase and luteal phase and also during various stages of pregnancy like early-pregnancy (d 61-83), mid-pregnancy (d 100-168) and late-pregnancy (d 239-246). Six animals at age of 3-7 years were used in each group. Lymphocytes were separated by density gradient centrifugation technique, and cultured for 72 hours. Then culture supernatant was used for measurement of NO by colourimetric assay based on the Griess reaction. Plasma levels of estradiol and progesterone were also measured by enzyme-immuno assay (EIA) to ascertain the stage of estrous cycle. Lymphocytic NO secretion during mid pregnancy (d 100-168) (35.36±1.18 µM nitrite) was significantly higher than follicular phase (26.60±3.71 µM nitrite, P

    REFERENCES

    1. Ali, M.; Buhimschi, I.; Chwalisz, K. and Garfield, R.E. (1997). Changes in expression of the nitric oxide synthease isoforms in rat uteus and cervix during pregnancy and parturition. Mol. Hum. Reprod. 3: 995-1003.
    2. Bellefontaine, N.; Hanchate, N.K.; Parkash, J.; Campagne, C.; de Seranno, S.; Clasadonte, J.; d’Anglemont de Tassigny, X. and Prevot, V. (2011) Nitric oxide as key mediator of neuron-to-neuron and endothelia-to-glia communication involved in the neuroendocrine control of reproduction. Neuroendocrinol. 93 (2): 74-89.
    3. Biswas, S.; Kabir, S.N. and Pal, A.K. (1998) The role of nitric oxide in the process of implantation in rats. J. Reprod. Fert. 114: 157-161.
    4. Bredt, D.S. and Snyder, S.H. (1994) Nitric oxide: A physiologic messenger molecule. Annu. Rev. Biochem. 63: 175-195.
    5. Dixit, V. D. and Parvizi, N. (2001) Pregnancy stimulates secretion of adrenocroticotropin and nitric oxide from peripheral bovine lymphocytes. Bol. Reprod. 64: 242-248.
    6. Dixit, V. D. and Parvizi, N. (2001b) Nitric oxide and the control of reproduction. Anim. Reprod. Sci. 65: 1-16.
    7. Glatz-Vinke, B. (1998) Lymphocytic hormone secretion: secretion and regulation of ACTH in pigs and GH and ACTH in cattle. Ph.D. Thesis, Vet. Med. School, Hannover, Germany.
    8. Goerner, C. (1995) Untersuchungen zur immuneendokrinen Intraction bein Schwein. Wachstumshormonsekretion bei Immunzellen. Ph.D. Thesis, Vet. Med. School, Hannover, Germany.
    9. Lee, S.H.; Acosta, T.J.; Yoshioka, S. and Okuda, K. (2009) Prostaglandin F2 alpha regulates the nitric oxide generating system in bovine luteal endothelial cells. J. Reprod. Dev. 55:418-424.
    10. Luo, Q.; Chen, X.J.; Ding, G.L.; Dong, M.Y. and Huang, H.F. (2010) Downregulative effects of nitric oxide on oocyte fertilization and embryo development: possible roles of nitric oxide in the pathogenesis of endometriosis-associated infertility. Cell Physiol. Biochem. 26 : 1023-1028.
    11. Motran, C.C.; Diaz, F. L.; Gruppi, A.; Slavin, D.; Chatton, B. and Bocco, J.L. (2002) Human pregnancy specific glycoprotein 1a (PSG1a) induces alternative activation in human and mouse monocytes and suppresses the accessory cell-dependent T cell proliferation. J. Leukoc. Biol. 72 : 512-521.
    12. Pancarci, S.M.; Gungor, O.; Atakisi, O.; Cigremis, Y.; Ari, U.C. and Bollwein H. (2011) Changes in follicular blood flow and nitric oxide levels in follicular fluid during follicular deviation in cows. Anim. Reprod. Sci. 123 : 149-156.
    13. Shukovski, L.; and Tsafriri, A. (1994) The involvement of nitric oxide in the ovulatory process in the rat. Endocrinol. 135: 2287-2290.
    14. Sirotkin, A.V. and Grossmann, R. (2003) Role of tyrosine kinase- and MAP kinase-dependent intracellular mechanisms in control of ovarian functions in the domestic fowl (Gallus domesticus) and in mediating effects of IGF-II. J. Reprod. Dev., 49: 99-106.
    15. Sladek, S.M. and Magness, R.R. and Conrad, K.P. (1997) Nitric oxide and pregnancy. Am. J. Physiol. 272 (2): R441-R463.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)