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Current IssueEditorial BoardOnline First ArticlesArchive

volume 47 issue 4 (august 2013) : 315-320

COMPUTER ASSISTED SPERM ANALYSIS OF FRESH AND FROZEN-THAWED HF X KANKREJ (F1) BULLS SEMEN AND THEIR INTERRELATIONSHIP

J
J.b. Patel1
A
A.j. Dhami*
1College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand-388 001, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Patel1 J.b., Dhami* A.j. (2025). COMPUTER ASSISTED SPERM ANALYSIS OF FRESH AND FROZEN-THAWED HF X KANKREJ (F1) BULLS SEMEN AND THEIR INTERRELATIONSHIP. Indian Journal of Animal Research. 47(4): 315-320. doi: .

ABSTRACT

A total of 30 good quality semen ejaculates from 5 mature bulls of HF x Kankrej (F1) breed were studied for sperm motility and velocity parameters of fresh and frozen-thawed spermatozoa using computer assisted sperm analyzer (CASA). The ejaculates immediately after collection in AV were assessed by CASA for fresh sperm motility and velocity parameters. A part of each ejaculate cryopreserved at -196°C in LN2 using standard Tris-citric acid-fructose-yolk-glycerol extender was thawed and assessed for post-thaw analysis of motility and velocity traits on CASA using via-dent stain.  The mean values of motile and progressively motile spermatozoa observed in fresh semen (74.73±1.71 and 51.57±2.61 %) were highly significantly (Ptozoa were significantly and positively interrelated. Thus, CASA appeared to be the fast, accurate and precise means of qualitative evaluation of the fresh and frozen-thawed bull semen.

REFERENCES

  1. Anel L., de Paz P., Alvarez M., Chamorro C.A., Boixo J.C., Manso A., Gonzalez M., Kaabi M. and Anel E. (2003). Field and in vitro assay of three methods for freezing ram semen. Theriogenology, 60: 1293-1308.
  2. Belorkar P.M., Dhami A.J., Derashri H.J. and Kodagali S.B. (1990). Studies on seminal and mensurational characteristics of spermatozoa and their interrelationships with freezability and fertility in crossbred bulls. PKV Res. J., 14: 165-173.
  3. Christensen P., Boelling D., Pedersen K.M., Korsgaard I.R. and Jensen J. (2005). Relationship between sperm viability as determined by flow cytometry and non-return rate of dairy bulls. J. Androl., 26: 98-106.
  4. David G., Serres C. and Jouannet P. (1981). Kinematics of human spermatozoa. Gamete Res., 4: 83-95.
  5. Doardo J., Hidalgo M., Munoz A. and Rodriguez I. (2009). Assessment of goat semen freezability according to the spermatozoa characteristics from fresh and frozen samples. Anim. Reprod. Sci., 112: 150-157.
  6. Flores E., Taberner E., Rivera M.M., Pena A., Rgau T., Miro J. and Rodriguez-Gil J.E. (2008). Effects of freezing/thawing on motile sperm subpopulations of boar and donkey ejaculates. Theriogenology, 70: 936-945.
  7. Hashida N.H., Nor Ashikin M.N.K., Khaironizam M.Z. and Abdullah R.B. (2013). Effects of freezing on sperm velocity in four different age groups of Jermasia buck. Indian J. Anim. Res., 47: 54-57.
  8. Kathiravan P., Kalatharan J., Edwin M.J. and Veerapandian C. (2008). Computer automated motion analysis of crossbred bull spermatozoa and its relationship with in vitro fertility in zona-free hamster oocytes: Anim. Reprod. Sci., 104: 9-17.
  9. Karthikeya G (2003). Computer automated motility and morphometric analysis of bull and buck spermatozoa. MVSc Thesis, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India.
  10. Knuth U.A., Neuwinger J. and Nieschlag E. (1989). Bias of routine semen analysis by uncontrolled changes is laboratory environment – detection by long term sampling of monthly means for quality control. Int. J. Androl., 12: 373- 383.
  11. Mandal D.K., Nagpaul P.K. and Gupta A.K. (2003). Motion characteristics of Murrah buffalo bull spermatozoa in various seasons and its relationship with functional integrity of the plasmallema. Theriogenology, 60: 349-358.
  12. Martinez-Pastor F., Garcia-Macias V., Alvarez M., Herrez P., Anel L. and De Paz P. (2005). Sperm subpopulations in Iberian red deer epididymal sperm and their changes through the cryopreservation process. Biol. Reprod., 72: 316-327.
  13. Mortimer D., Keel B.A. and Webster B.W. (1990). Objective analysis of sperm motility and kinematics. In: Handbook of the Laboratory Diagnosis and Treatment of Infertility. 1st edn., CRC Press, Boca Raton, Fla., pp. 97–133.
  14. Moses D.F., de las Heras M.A., Valcarcel A., Perez L. and Baldassare H. (1995). Use of computerized motility analyzer for the evaluation of frozen-thawed ram spermatozoa. Andrologia, 27: 25-29.
  15. Muino R., Pena A.I., Rodrigez A., Tamargo C. and Hidalgo C.O. (2009). Effects of cryopreservation on the motile sperm subpopulation in semen from Asturiana de los Valles bulls. Theriogenology, 72: 860-868.
  16. Patel K.V., Dhami A.J. and Kodagali S.B. (1989). Seminal characteristics and their interrelationships in K x J and K x HF crossbred bulls. Indian Vet. J., 66: 737-743.
  17. Patel N.P., Patel S.H. and Devanand C.P. (2010). Comparative studies on the assessment of the bovine sperm motility by conventional method and CASA System (HTM IVOS). Proc. XXVI Annual Convention of ISSAR and International Symposium, GBPUA & T, Pantnagar, India, 10-12 Nov., p. 11.
  18. Patil S.B., Jayaprakash D. and Shivaji S. (1998). Cryopreservation of semen of tigers and lions: computerized analysis of the motility parameters of the spermatozoa. Curr. Sci., 75: 930-935.
  19. Ramachandran N., Verma G.S., Gupta A.K., Raina V.S., Mohanty T.K. and Singh M.K. (2006). In vitro assessment of seminal traits and fertility parameters in fresh semen of Sahiwal bulls. Indian J. Anim. Reprod., 27: 100-103.
  20. Ramachandran N., Verma G.S., Gupta A.K., Raina V.S., Mohanty T.K. and Singh M.K. (2007). Prediction of fertility based on frozen-thawed seminal traits in Sahiwal bulls. Indian J. Anim. Sci., 77: 884-887.
  21. Raval R.J. and Dhami A.J (2006). Physico-biochemical attributes of semen and their interrelationships in triple crossbred (HF x J x K) bulls. Int. J. Cow Sci., 2: 34-40.
  22. Snedecor G.W. and Cochran W.G. (1980). Statistical Methods. 14th edn. Oxford and IBH Publishing House, New Delhi, India.
  23. Viswanath R. and Shannon P. (1997). Do sperm cells age? A review of the physiological change in sperm during storage at ambient temperature. Reprod. Fert. Dev., 9: 321-331.
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    volume 47 issue 4 (august 2013) : 315-320

    COMPUTER ASSISTED SPERM ANALYSIS OF FRESH AND FROZEN-THAWED HF X KANKREJ (F1) BULLS SEMEN AND THEIR INTERRELATIONSHIP

    J
    J.b. Patel1
    A
    A.j. Dhami*
    1College of Veterinary Science and Animal Husbandry Anand Agricultural University, Anand-388 001, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Patel1 J.b., Dhami* A.j. (2025). COMPUTER ASSISTED SPERM ANALYSIS OF FRESH AND FROZEN-THAWED HF X KANKREJ (F1) BULLS SEMEN AND THEIR INTERRELATIONSHIP. Indian Journal of Animal Research. 47(4): 315-320. doi: .

    ABSTRACT

    A total of 30 good quality semen ejaculates from 5 mature bulls of HF x Kankrej (F1) breed were studied for sperm motility and velocity parameters of fresh and frozen-thawed spermatozoa using computer assisted sperm analyzer (CASA). The ejaculates immediately after collection in AV were assessed by CASA for fresh sperm motility and velocity parameters. A part of each ejaculate cryopreserved at -196°C in LN2 using standard Tris-citric acid-fructose-yolk-glycerol extender was thawed and assessed for post-thaw analysis of motility and velocity traits on CASA using via-dent stain.  The mean values of motile and progressively motile spermatozoa observed in fresh semen (74.73±1.71 and 51.57±2.61 %) were highly significantly (Ptozoa were significantly and positively interrelated. Thus, CASA appeared to be the fast, accurate and precise means of qualitative evaluation of the fresh and frozen-thawed bull semen.

    REFERENCES

    1. Anel L., de Paz P., Alvarez M., Chamorro C.A., Boixo J.C., Manso A., Gonzalez M., Kaabi M. and Anel E. (2003). Field and in vitro assay of three methods for freezing ram semen. Theriogenology, 60: 1293-1308.
    2. Belorkar P.M., Dhami A.J., Derashri H.J. and Kodagali S.B. (1990). Studies on seminal and mensurational characteristics of spermatozoa and their interrelationships with freezability and fertility in crossbred bulls. PKV Res. J., 14: 165-173.
    3. Christensen P., Boelling D., Pedersen K.M., Korsgaard I.R. and Jensen J. (2005). Relationship between sperm viability as determined by flow cytometry and non-return rate of dairy bulls. J. Androl., 26: 98-106.
    4. David G., Serres C. and Jouannet P. (1981). Kinematics of human spermatozoa. Gamete Res., 4: 83-95.
    5. Doardo J., Hidalgo M., Munoz A. and Rodriguez I. (2009). Assessment of goat semen freezability according to the spermatozoa characteristics from fresh and frozen samples. Anim. Reprod. Sci., 112: 150-157.
    6. Flores E., Taberner E., Rivera M.M., Pena A., Rgau T., Miro J. and Rodriguez-Gil J.E. (2008). Effects of freezing/thawing on motile sperm subpopulations of boar and donkey ejaculates. Theriogenology, 70: 936-945.
    7. Hashida N.H., Nor Ashikin M.N.K., Khaironizam M.Z. and Abdullah R.B. (2013). Effects of freezing on sperm velocity in four different age groups of Jermasia buck. Indian J. Anim. Res., 47: 54-57.
    8. Kathiravan P., Kalatharan J., Edwin M.J. and Veerapandian C. (2008). Computer automated motion analysis of crossbred bull spermatozoa and its relationship with in vitro fertility in zona-free hamster oocytes: Anim. Reprod. Sci., 104: 9-17.
    9. Karthikeya G (2003). Computer automated motility and morphometric analysis of bull and buck spermatozoa. MVSc Thesis, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India.
    10. Knuth U.A., Neuwinger J. and Nieschlag E. (1989). Bias of routine semen analysis by uncontrolled changes is laboratory environment – detection by long term sampling of monthly means for quality control. Int. J. Androl., 12: 373- 383.
    11. Mandal D.K., Nagpaul P.K. and Gupta A.K. (2003). Motion characteristics of Murrah buffalo bull spermatozoa in various seasons and its relationship with functional integrity of the plasmallema. Theriogenology, 60: 349-358.
    12. Martinez-Pastor F., Garcia-Macias V., Alvarez M., Herrez P., Anel L. and De Paz P. (2005). Sperm subpopulations in Iberian red deer epididymal sperm and their changes through the cryopreservation process. Biol. Reprod., 72: 316-327.
    13. Mortimer D., Keel B.A. and Webster B.W. (1990). Objective analysis of sperm motility and kinematics. In: Handbook of the Laboratory Diagnosis and Treatment of Infertility. 1st edn., CRC Press, Boca Raton, Fla., pp. 97–133.
    14. Moses D.F., de las Heras M.A., Valcarcel A., Perez L. and Baldassare H. (1995). Use of computerized motility analyzer for the evaluation of frozen-thawed ram spermatozoa. Andrologia, 27: 25-29.
    15. Muino R., Pena A.I., Rodrigez A., Tamargo C. and Hidalgo C.O. (2009). Effects of cryopreservation on the motile sperm subpopulation in semen from Asturiana de los Valles bulls. Theriogenology, 72: 860-868.
    16. Patel K.V., Dhami A.J. and Kodagali S.B. (1989). Seminal characteristics and their interrelationships in K x J and K x HF crossbred bulls. Indian Vet. J., 66: 737-743.
    17. Patel N.P., Patel S.H. and Devanand C.P. (2010). Comparative studies on the assessment of the bovine sperm motility by conventional method and CASA System (HTM IVOS). Proc. XXVI Annual Convention of ISSAR and International Symposium, GBPUA & T, Pantnagar, India, 10-12 Nov., p. 11.
    18. Patil S.B., Jayaprakash D. and Shivaji S. (1998). Cryopreservation of semen of tigers and lions: computerized analysis of the motility parameters of the spermatozoa. Curr. Sci., 75: 930-935.
    19. Ramachandran N., Verma G.S., Gupta A.K., Raina V.S., Mohanty T.K. and Singh M.K. (2006). In vitro assessment of seminal traits and fertility parameters in fresh semen of Sahiwal bulls. Indian J. Anim. Reprod., 27: 100-103.
    20. Ramachandran N., Verma G.S., Gupta A.K., Raina V.S., Mohanty T.K. and Singh M.K. (2007). Prediction of fertility based on frozen-thawed seminal traits in Sahiwal bulls. Indian J. Anim. Sci., 77: 884-887.
    21. Raval R.J. and Dhami A.J (2006). Physico-biochemical attributes of semen and their interrelationships in triple crossbred (HF x J x K) bulls. Int. J. Cow Sci., 2: 34-40.
    22. Snedecor G.W. and Cochran W.G. (1980). Statistical Methods. 14th edn. Oxford and IBH Publishing House, New Delhi, India.
    23. Viswanath R. and Shannon P. (1997). Do sperm cells age? A review of the physiological change in sperm during storage at ambient temperature. Reprod. Fert. Dev., 9: 321-331.
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