Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 47 issue 3 (june 2013) : 265-267

DETERMINATION OF ALLELES OF THE OVINE PRNP GENE USING PCR-SINGLE STRAND CONFORMATIONAL POLYMORPHISM ANALYSIS IN MALPURA SHEEP

Sharda Choudhary*1, Neelam Gupta2
1National Bureau of Animal Genetic Resources, Karnal-132 001, India

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Cite article:- Choudhary*1 Sharda, Gupta2 Neelam (2024). DETERMINATION OF ALLELES OF THE OVINE PRNP GENE USING PCR-SINGLE STRAND CONFORMATIONAL POLYMORPHISM ANALYSIS IN MALPURA SHEEP. Indian Journal of Animal Research. 47(3): 265-267. doi: .

ABSTRACT

SSCP polymorphism was detected of ovine PRNP coding region exon-3 in 52 blood samples from Malpura sheep breed (Ovis aries) of semi-arid region of India. Three PCR-SSCP amplicons with different nucleotide base pairs were screened. In primer set I covering 19-373 nucleotide showed 6 types of haplotype, primer set 2 covering nucleotide region from 354-711 showed 9 types of haplotype (A-I) and primer set 3 nucleotide region from 692-1001 showed 9 types of haplotypes. The data will help for the detection of SNP markers related to disease resistance or susceptibility in India.

REFERENCES

  1. Bassam BJ G, Catano A and Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80-83.
  2. Choudhary S, Gupta N and Gupta S C. (2012). Identification of SNPs in PRNP gene in Karnah sheep breed of India. Indian J. Anim. Sci. 82: 609–614.
  3. Dawson M, Hoinville U, Hosie BD and Hunter N (1998) Guid­ance on the use of PrP genotyping as an aid to the control of clinical scrapie. Vet Rec 142:623-625.
  4. DeSilva U, Guo X, Kufer DM, Fernando SC, Pillai ATV, Najar FZ, So S, Fitch GQ and Roe BA (2003) Allelic variation of ovine prion protein gene (PRNP) in Oklahoma sheep. Cytogenet. Genome Res. 102:89-94.
  5. Goldmann W, Martin T, Foster J, Hughes S, Smith G, Hughes K, Dawson M and Hunter N (1996) Novel polymor­phisms in the caprine PrP gene: A codon 142 muta­tion associated with scrapie incubation period. J Gen Virol. 77:2885-2891.
  6. Hunter N, Foster JD, Goldmann W, Stear MJ and Hope J and Bostock C (1996) Natural scrapie in a closed flock of Che­viot sheep occurs only in specific PrP genotypes. Arch Virol. 141:809-824.
  7. Hunter N, Goldmann W, Marshall E and O’Neill G (2000) Sheep and goats: natural and experimental TSEs and fac­tors influencing incidence of disease. Arch Virol. 181-188.
  8. Mead S, Stumpf MPH, Whitfield J, Beck JA, Poulter M, Campbell T, Uphill J, Goldstein D, Alpers M, Fisher EMC and Collinge J (2003) Balancing selection at the prion protein gene consistent with prehistoric ku­rulike epidemics. Science. 300:640-643.
  9. Prusiner, S.B. (1996). Molecular biology and pathogenesis of prion diseases. Trends Biochem. Sci. 21(12): 482-487.
  10. Sambrook J, Fritsch EF and Maniatis T (1989) In Molecular Cloning: A Laboratory Manual 2nd ed, Cold Spring Harbour, Cold Spring Laboratory Press, NY PP 1.21.
  11. Zhou H, Hicfford JGH, and Fang Q (2005) Technical Note: determination of alleles of the ovine PrNP gene using PCR- Single strand conformational polymorphism analysis. J. Anim. Sci. 83: 745-749.
  12. Zlotnik I and Katiyar RD (1961) The occurrence of scrapie disease in sheep of the remote Himalayan foothills. Vet Rec. 73:543-4.
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