volume 47 issue 3 (june 2013) : 265-267
DETERMINATION OF ALLELES OF THE OVINE PRNP GENE USING PCR-SINGLE STRAND CONFORMATIONAL POLYMORPHISM ANALYSIS IN MALPURA SHEEP
1National Bureau of Animal Genetic Resources,
Karnal-132 001, India
Published In
Indian Journal of Animal Research
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volume 47 issue 3 (june 2013) : 265-267
DETERMINATION OF ALLELES OF THE OVINE PRNP GENE USING PCR-SINGLE STRAND CONFORMATIONAL POLYMORPHISM ANALYSIS IN MALPURA SHEEP
1National Bureau of Animal Genetic Resources,
Karnal-132 001, India
ABSTRACT
SSCP polymorphism was detected of ovine PRNP coding region exon-3 in 52 blood samples from Malpura sheep breed (Ovis aries) of semi-arid region of India. Three PCR-SSCP amplicons with different nucleotide base pairs were screened. In primer set I covering 19-373 nucleotide showed 6 types of haplotype, primer set 2 covering nucleotide region from 354-711 showed 9 types of haplotype (A-I) and primer set 3 nucleotide region from 692-1001 showed 9 types of haplotypes. The data will help for the detection of SNP markers related to disease resistance or susceptibility in India.
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In this Article
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Published In
Indian Journal of Animal Research