volume 44 issue 4 (december 2010) : 280 - 284
BACTERIOLOGY OF SUB CLINICAL MASTITIS AND ANTIBIOGRAM OF ISOLATES RECOVERED FROM CROSS BRED COWS
1Dept. of Dairy Science, Veterinary College & Research Institute,
Namakkal-637 002, India.
ABSTRACT
A total of 178 milk samples from apparently healthy lactating crossbred cows (98
Jersey cross and 80 Holstein Friesian cross) were screened for sub-clinical mastitis. The
physical examination of sub clinical mastitic milk revealed no changes in colour, consistency
and odour. The chemical examination revealed increased pH, somatic cell count and chloride
content and positive scores on modified california mastitis test. Out of 98 Jersey crosses
and 80 Holstein Friesian crosses which were subjected to examination 30 Jersey crosses(30.61%)
and Holstein Friesian crosses 27(33.75%) were found to be affected with subclinical mastitis
with a cumulative incidence of 32.02% in cross bred cows. The isolated pathogens from
sub-clinical cases and their relative frequencies were: Staphylococcus sp. (38.59%),
Streptococcus sp. (35.08%), Escherichia coli (12.28%), Corynebacterium pyogens (8.77%)
and Bacillus sp. (5.26%). The in vitro antibiogram revealed Ceftriaxone(92.66%),
Enrofloxacin(89.83%), Kanamycin (88.13%), Ciprofloxacin (82.3%) were most effective,
Gentamicin (63.16%) and Chloramphenicol (55.93%) were moderately effective and
Tetracycline (32.20%), Ampicillin(30.50%) and Erythromycin (28.81%) were the least effective
drugs.
Jersey cross and 80 Holstein Friesian cross) were screened for sub-clinical mastitis. The
physical examination of sub clinical mastitic milk revealed no changes in colour, consistency
and odour. The chemical examination revealed increased pH, somatic cell count and chloride
content and positive scores on modified california mastitis test. Out of 98 Jersey crosses
and 80 Holstein Friesian crosses which were subjected to examination 30 Jersey crosses(30.61%)
and Holstein Friesian crosses 27(33.75%) were found to be affected with subclinical mastitis
with a cumulative incidence of 32.02% in cross bred cows. The isolated pathogens from
sub-clinical cases and their relative frequencies were: Staphylococcus sp. (38.59%),
Streptococcus sp. (35.08%), Escherichia coli (12.28%), Corynebacterium pyogens (8.77%)
and Bacillus sp. (5.26%). The in vitro antibiogram revealed Ceftriaxone(92.66%),
Enrofloxacin(89.83%), Kanamycin (88.13%), Ciprofloxacin (82.3%) were most effective,
Gentamicin (63.16%) and Chloramphenicol (55.93%) were moderately effective and
Tetracycline (32.20%), Ampicillin(30.50%) and Erythromycin (28.81%) were the least effective
drugs.
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Indian Journal of Animal Research
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