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Current IssueEditorial BoardOnline First ArticlesArchive

volume 44 issue 4 (december 2010) : 265 - 269

CHARACTERIZATION OF PASTEURELLA MULTOCIDA ISOLATED FROM PNEUMONIC PIGS OF ASSAM.

M
M.P. Hazarika
N
N.N. Barman
S
S. George
R
R.K. Sharma
1Department of Microbiology, College of Veterinary Science. Assam Agricultural University, Khannapara, Guwahati- 781 022, India.

  • Submitted|

  • First Online |

  • doi

Cite article:- Hazarika M.P., Barman N.N., George S., Sharma R.K. (2025). CHARACTERIZATION OF PASTEURELLA MULTOCIDA ISOLATED FROM PNEUMONIC PIGS OF ASSAM.. Indian Journal of Animal Research. 44(4): 265 - 269. doi: .

ABSTRACT

Characterization of pig isolates of Pasteurella multocida has been carried out on the basis of PCR
analysis, mouse bio-assay and electrophoretic migration pattern. Out of 20 swine isolates of P. multocida
two isolates (LKD and DP) caused 100 per cent mortality in the inoculated mice. Comparing with
that of P52 strain, mean death time of LKD strain was lower. The molecular weight of the sonicated
extracts of different swine isolates were not homogenous and ranged from 91.2 kDa to 16.6 kDa. While
in case of P52 reference strain molecular weights ranged from 83.2 kDa to 18.2 kDa. There were 18
bands in pig isolates and 17 protein bands in P52 strain. Study revealed that the pig isolate (LKD) was
more pathogenic than that of P52 strain and differed in number and migration of protein bands.

REFERENCES

  1. Chandrasekaran, S. and Yeap, P. C. (1982). Br. Vet. J. 138 : 332.
  2. Cruickshank, R., Duguid, J. P., Marmion, B. P. and Swain, R.H.A. (1975). Medical Micrbiology, 12th ed., Vol. II,
  3. Churchill Living stone, London, U.K.
  4. Curtis, P. E. (1985). Pasteurella multocida. In : Isolation and Identification of Microorganisms of Medical and Veterinary
  5. Importance. [(C. H. Collins and J. M. Grange (Eds)], Academic Press, London. p. 43.
  6. Djordjenic, S. P., Eamens, G.J., Ha, H., Walker, M. J. and Chin, J. C. (1998). J Med Microbiol 47 : 679.
  7. Dwivedi, P. N. and Sodhi, S.S. (1988). Livestock Adviser. 13 (10) :48.
  8. Dziva,F., Christensen ,H., Olsen ,J.E., and Mohan,K. (2001).Vet. Microbiol. 82(4):361-372.
  9. Gamage, L.N.A., Wijewardana, T.G.,Bastiansz H.L.G. and Vipulasiri, A.A. (1995). Vet J 42 :15-19.
  10. Joshi, V. B., Borkakoti, P. and Baxi, K. K. (1990). Indian J Comp. Microbiol. Immunol. Infect. Dis. 11 (3&4) : 149.
  11. Kumar, P. N. and Uppal. P. K. (1986). Indian J Comp. Microbiol. Immunol. Infect. Dis. 7 (2&3) : 112.
  12. Kalorey D.R., Yuvaraj S, Vanjari S.S. , Gunjal P.S., Dhanawade N.B. , Barbuddhe S.B. and Bhandarkar A.G.(2007).
  13. Comp. Immunol, Microbiol. Infect. Dis..31: 459.
  14. Kumar,A.A., Harbola,P.C, Rimler,R.B. and Kumar,P. (1996). Indian J Comp Microbiol Immunol Infect Dis 17 : 120.
  15. Kumar,A.A., Shivachandra,S.B., Biswas,A., Singh,V.P. and Srivastava,S.K. (2004). Vet Res Commun 28: 657.
  16. Laemmli, U. K. (1970). Nature. 227 : 680-685.
  17. Monoharan, S., Jayaprakasan, V., Pillai, R. M. and Sulochana, S. (1997). Indian Vet. J. 74 : 1.
  18. Muller, H. E. and Krasenmann, C. (1974). Zntl. Bakteriol. Parasitknde Infekt. Hyg. Abt. Orig. 229A : 391.
  19. Muruyama, T., Kanzaki, M., Usami, T. and Mizutani, H. (1987). Ann. Rep. Tokyo Metr. Res. Lab P. H. 38 : 42.
  20. Singh, R. and Goel, M. C. (2002). Indian J. Anim. Sci. 72 (8) : 625.
  21. Sunder, J. and Kumar, A. A. (1999). Indian J Anim Sci. 69 (10) : 753.
  22. Sunder, J. and Kumar, A. A. (2000). Indian J. Anim Sci. 70 (3) : 235.
  23. Verma N.D. (1988). Vet Rec 123:63.
  24. Townsend, K.M. , Frost A.J., Lee C.W., Papadimitriou J.M., and Dawkins, H.J. (1998). J Clin Microbiol 36:1096.
  25. Townsend K.M., Boyce J.D., Chung J.Y., Frost A.J., & Adler B.(2001).J. Clin.Microbiol. 39:924-929
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    volume 44 issue 4 (december 2010) : 265 - 269

    CHARACTERIZATION OF PASTEURELLA MULTOCIDA ISOLATED FROM PNEUMONIC PIGS OF ASSAM.

    M
    M.P. Hazarika
    N
    N.N. Barman
    S
    S. George
    R
    R.K. Sharma
    1Department of Microbiology, College of Veterinary Science. Assam Agricultural University, Khannapara, Guwahati- 781 022, India.

    • Submitted|

    • First Online |

    • doi

    Cite article:- Hazarika M.P., Barman N.N., George S., Sharma R.K. (2025). CHARACTERIZATION OF PASTEURELLA MULTOCIDA ISOLATED FROM PNEUMONIC PIGS OF ASSAM.. Indian Journal of Animal Research. 44(4): 265 - 269. doi: .

    ABSTRACT

    Characterization of pig isolates of Pasteurella multocida has been carried out on the basis of PCR
    analysis, mouse bio-assay and electrophoretic migration pattern. Out of 20 swine isolates of P. multocida
    two isolates (LKD and DP) caused 100 per cent mortality in the inoculated mice. Comparing with
    that of P52 strain, mean death time of LKD strain was lower. The molecular weight of the sonicated
    extracts of different swine isolates were not homogenous and ranged from 91.2 kDa to 16.6 kDa. While
    in case of P52 reference strain molecular weights ranged from 83.2 kDa to 18.2 kDa. There were 18
    bands in pig isolates and 17 protein bands in P52 strain. Study revealed that the pig isolate (LKD) was
    more pathogenic than that of P52 strain and differed in number and migration of protein bands.

    REFERENCES

    1. Chandrasekaran, S. and Yeap, P. C. (1982). Br. Vet. J. 138 : 332.
    2. Cruickshank, R., Duguid, J. P., Marmion, B. P. and Swain, R.H.A. (1975). Medical Micrbiology, 12th ed., Vol. II,
    3. Churchill Living stone, London, U.K.
    4. Curtis, P. E. (1985). Pasteurella multocida. In : Isolation and Identification of Microorganisms of Medical and Veterinary
    5. Importance. [(C. H. Collins and J. M. Grange (Eds)], Academic Press, London. p. 43.
    6. Djordjenic, S. P., Eamens, G.J., Ha, H., Walker, M. J. and Chin, J. C. (1998). J Med Microbiol 47 : 679.
    7. Dwivedi, P. N. and Sodhi, S.S. (1988). Livestock Adviser. 13 (10) :48.
    8. Dziva,F., Christensen ,H., Olsen ,J.E., and Mohan,K. (2001).Vet. Microbiol. 82(4):361-372.
    9. Gamage, L.N.A., Wijewardana, T.G.,Bastiansz H.L.G. and Vipulasiri, A.A. (1995). Vet J 42 :15-19.
    10. Joshi, V. B., Borkakoti, P. and Baxi, K. K. (1990). Indian J Comp. Microbiol. Immunol. Infect. Dis. 11 (3&4) : 149.
    11. Kumar, P. N. and Uppal. P. K. (1986). Indian J Comp. Microbiol. Immunol. Infect. Dis. 7 (2&3) : 112.
    12. Kalorey D.R., Yuvaraj S, Vanjari S.S. , Gunjal P.S., Dhanawade N.B. , Barbuddhe S.B. and Bhandarkar A.G.(2007).
    13. Comp. Immunol, Microbiol. Infect. Dis..31: 459.
    14. Kumar,A.A., Harbola,P.C, Rimler,R.B. and Kumar,P. (1996). Indian J Comp Microbiol Immunol Infect Dis 17 : 120.
    15. Kumar,A.A., Shivachandra,S.B., Biswas,A., Singh,V.P. and Srivastava,S.K. (2004). Vet Res Commun 28: 657.
    16. Laemmli, U. K. (1970). Nature. 227 : 680-685.
    17. Monoharan, S., Jayaprakasan, V., Pillai, R. M. and Sulochana, S. (1997). Indian Vet. J. 74 : 1.
    18. Muller, H. E. and Krasenmann, C. (1974). Zntl. Bakteriol. Parasitknde Infekt. Hyg. Abt. Orig. 229A : 391.
    19. Muruyama, T., Kanzaki, M., Usami, T. and Mizutani, H. (1987). Ann. Rep. Tokyo Metr. Res. Lab P. H. 38 : 42.
    20. Singh, R. and Goel, M. C. (2002). Indian J. Anim. Sci. 72 (8) : 625.
    21. Sunder, J. and Kumar, A. A. (1999). Indian J Anim Sci. 69 (10) : 753.
    22. Sunder, J. and Kumar, A. A. (2000). Indian J. Anim Sci. 70 (3) : 235.
    23. Verma N.D. (1988). Vet Rec 123:63.
    24. Townsend, K.M. , Frost A.J., Lee C.W., Papadimitriou J.M., and Dawkins, H.J. (1998). J Clin Microbiol 36:1096.
    25. Townsend K.M., Boyce J.D., Chung J.Y., Frost A.J., & Adler B.(2001).J. Clin.Microbiol. 39:924-929
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