CHARACTERIZATION OF PASTEURELLA MULTOCIDA ISOLATED FROM PNEUMONIC PIGS OF ASSAM.

Article Id: ARCC1258 | Page : 265 - 269
Citation :- CHARACTERIZATION OF PASTEURELLA MULTOCIDA ISOLATED FROM PNEUMONIC PIGS OF ASSAM..Indian Journal Of Animal Research.2010.(44):265 - 269
M.P. Hazarika, N.N. Barman, S. George and R.K. Sharma
Address : Department of Microbiology, College of Veterinary Science. Assam Agricultural University, Khannapara, Guwahati- 781 022, India.

Abstract

Characterization of pig isolates of Pasteurella multocida has been carried out on the basis of PCR
analysis, mouse bio-assay and electrophoretic migration pattern. Out of 20 swine isolates of P. multocida
two isolates (LKD and DP) caused 100 per cent mortality in the inoculated mice. Comparing with
that of P52 strain, mean death time of LKD strain was lower. The molecular weight of the sonicated
extracts of different swine isolates were not homogenous and ranged from 91.2 kDa to 16.6 kDa. While
in case of P52 reference strain molecular weights ranged from 83.2 kDa to 18.2 kDa. There were 18
bands in pig isolates and 17 protein bands in P52 strain. Study revealed that the pig isolate (LKD) was
more pathogenic than that of P52 strain and differed in number and migration of protein bands.

Keywords

Pasteurella multocida Pneumonic pigs Swine.

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