Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 47 issue 2 (april 2013) : 164-167

GENETIC VARIABILITY OF BOLA-DRB3.2 GENE IN KENKATHA BREED OF CATTLE ASSESSED BY PCR-SSCP

Rakesh Ranjan*, C.D. Bhong1, K.N. Chavan2, S.N.S. Parmar, C.G. Joshi3
1Animal Biotechnology Centre, MPPCVV, Jabalpur 482 004, India
  • Submitted|

  • First Online |

  • doi

Cite article:- Ranjan* Rakesh, Bhong1 C.D., Chavan2 K.N., Parmar S.N.S., Joshi3 C.G. (2024). GENETIC VARIABILITY OF BOLA-DRB3.2 GENE IN KENKATHA BREED OF CATTLE ASSESSED BY PCR-SSCP. Indian Journal of Animal Research. 47(2): 164-167. doi: .
Present study was conducted to investigate BoLA (Bovine leucocyte antigen) gene polymorphism in indigenous draft Kenkatha (n=52) breed of cattle at DRB3.2 loci by PCR-SSCP (Single strand conformational polymorphism). This BoLA region encodes cell surface glycoprotein, which binds to foreign antigens of T lymphocytes that play central role in the induction and regulation of adaptive immunity. A  PCR-SSCP of 284bp amplicon of BoLA-DRB3.2 gene revealed 13 SSCP patterns. This suggests that exon 2 region of BoLA-DRB3 is highly polymorphic in nature. This may be due to natural selection on a BoLA allele pool, leading to the development of resistance to local infectious diseases.
  1. Beever, J.E, George, P. D, Fernando, R.L, Stormont, C.J. and Lewin, H.A (1990) Associations between genetic markers and growth and carcass traits in a paternal half-sib family of Angus cattle, J. Anim. Sci, 68:337-344
  2. Behl, J.D, Verma, N.K, Behl, R, Mukesh, M and Ahlawat, S.P. S (2007) Characterization of genetic polymorphism of the bovine lymphocyte antigen DRB3.2 locus in Kankrej cattle (Bos indicus), J Dairy Sci , 90:2997-3001
  3. Ellis, S.A and Ballingall, K.T (1999) Cattle MHC: evolution in action Immunol. Rev, 167:159-168
  4. Germain, R.N (1994) MHC-dependent antigen processing and peptide presentation: providing ligands for T lymphocyte activation, Cell, 76:287-299
  5. John, S.W, Weitzner, G, Rozen, R, and Scriver, C.R (1991) A rapid procedure for extracting genomic DNA from leukocytes, Nucleic Acids Res, 19: 408
  6. Moon, J.O, Park, Y. S, Lee, K.O, Ahn, B.H (1997) DNA polymorphism analysis of BoLA DRB3 gene between Hanwoo and Holstein in Korea, Kor. J. Genet, 19:343
  7. Nivsarkar, A.E, Vij, P. K. and Tantia, M.S (2000) Animal genetic resources of India: Cattle and Buffalo. ICAR, India
  8. Ranjan, R, Bhong, C.D, Chavan, K.N, Parmar, S.N.S and Joshi, C.G (2010) Polymorphism Study of Locus DRB3.2 Malvi breed of cattle, J. Bombay Vet. College 18(1):15-17
  9. Takeshima, S, Nakai, Y, Ohta, M and Aida, Y (2002) Characterization of DRB3 alleles in the MHC of Japanese shorthorn cattle by polymerase chain reaction-sequence based typing, J. Dairy Sci, 85:1630-1632
  10. Sambrook, J and Russell, D.W (2001) Molecular Cloning: A Laboratory Manual, Cold Spring Harbour Laboratory, Cold Spring Harbour, New York
  11. http://www.projects.roslin.ac.uk/ bola/reports.html
  12. (http://mplivestock.com/events.html).

Editorial Board

View all (0)