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Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Indian Journal of Animal Research, volume 47 issue 1 (february 2013) : 66-69

PHYLOGENETIC ANALYSIS OF EXON II OF MELATONIN RECEPTOR 1A (MTNR1A) GENE IN MERINO CROSS EWES IN KASHMIR

T. Ahmad*, T.A.S. Ganai, A.K. Pandey1, A. Magotra2
1Division of Animal Genetics and Breeding, SKUAST-K, Srinagar-190 006, India

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Cite article:- Ahmad* T., Ganai T.A.S., Pandey1 A.K., Magotra2 A. (2025). PHYLOGENETIC ANALYSIS OF EXON II OF MELATONIN RECEPTOR 1A (MTNR1A) GENE IN MERINO CROSS EWES IN KASHMIR. Indian Journal of Animal Research. 47(1): 66-69. doi: .

ABSTRACT

The seasonality of sheep reproduction is a major limiting factor in round the year lamb production under temperate climate. The objective of this study was to analyse allelic variants of exon II of melatonin receptor (MTNR1A) gene in Merino cross ewes by phylogenetic analysis. The genotypes of the MTNR1A were determined by PCR-RFLP. Genomic DNA was extracted from 49 Merino cross ewes, PCR reaction was carried out and PCR products were digested by the Mnl1 restriction enzyme and placed into M/M, M/m or m/m genotypes. Sequencing of the representative samples showing different RFLP patterens were got done both in forward as well as reverse direction. The obtained nucleotide sequences of different alleles of exon II fragment of the gene were analysed to generate phylogenetic relationship, the homology (percentage similarity) and the relative distance (divergence) with sheep (Acc. No’s U14109, AF045219), goat (Acc. No AF419334), cattle (Acc. No EU716174), buffalo (Acc. No GU817415) and human (Acc. No AB029933). The results confirmed that the diversity in the amplified fragment is in consonance with the phylogenty of species. This study also provided evidence that Merino cross have variability in MTNR1A locus. Therefore, this locus can be used as a marker for the reproduction trait in selection programs.

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