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The Effect of Growth Conditions on Mycelial Run of Oyster Mushrooms spp. (Pleurotus spp.): Implication for Agricultural Practices

DOI: 10.18805/BKAP470    | Article Id: BKAP470 | Page : 137-143
Citation :- The Effect of Growth Conditions on Mycelial Run of Oyster Mushrooms spp. (Pleurotus spp.): Implication for Agricultural Practices.Bhartiya Krishi Anusandhan Patrika.2022.(37):137-143
Kartik Charan Lenka, Bandana Padhan, Naina Pradhan, Truptimayee Mantry, Romalin Sahu, S. Venkatlaxmi miki.bandana@gmail.com
Address : Department of Biotechnology, School of Life Science and Biotechnology, Adamas University, Kolkata-700 126, West Bengal, India.
Submitted Date : 21-02-2022
Accepted Date : 12-05-2022

Abstract

Background: The Pleurotus genus is one of the most investigated white-rot fungus due to its excellent ligninolytic characteristics. It is a tasty mushroom that also has a number of biological impacts due to the presence of essential bioactive compounds. Many usual fermentation parameters affect lignocellulolytic enzymes in basidiomycete fungi, such as medium composition, carbon-to-nitrogen ratio, pH, temperature, air composition etc. The survival and multiplication of Pleurotus mushrooms is dependent on a number of factors, which may function independently or interactive effect on the growth of mushroom. Grasp the difficulty of treating Pleurotus species mushrooms necessitates a fundamental understanding of their physical, chemical and biological characteristics.
Methods:  In this study, the effects of culture media, temperature, carbon sources, nitrogen sources, grain sources and agricultural waste as lignocellulosic substrate on the mycelium growth of five species of Pleurotus species for spawn production were studied. Several trials were designed to evaluate factors affecting mycelium growth of five oyster mushroom species. The experiments were conducted during October to February in the year 2019 to 2021. The experiments were carried out in three replications per treatment.
Result: The results of the experiment indicated that potato dextrose agar (PDA) and Malt extract agar (MEA) were the most suitable media for the mycelium growth of all oyster mushrooms. The optimal temperature for mycelium growth of all the oyster mushroom species was obtained at 25±2oC. Mycelium growth of all the studied oyster mushroom was improved by carbon sources such as dextrose and glucose. The nitrogen source such as ammonium chloride and ammonium sulfate also gave the greatest values in mycelium colony diameter of mushroom. Wheat was found to be favourable for mycelium growth of all the oyster mushroom species. In addition, paddy straw and sugarcane residue were selected as suitable lignocellulosic substrate sources for mycelium growth of all the studied oyster mushrooms.

Keywords

Biological efficiency Mycelium diameter Nutritional condition Oyster mushroom Pleurotus spp

References

  1. Atri, N.S., Upadhyay, R.C., Kumari, B. (2012). Comparative account of vitamin C content in Termitophilous and Lepiotoid mushrooms of Northwest India. African Journal of Basic and Applied Science. 4(4): 124-127.
  2. Bellettini, M. B., Fiorda, F.A., Maieves, H.A., Teixeira, G.L., Avila, S., Hornung, P.S., Ju´nior A.M., Ribani, R.H. (2019). Factors affecting mushroom Pleurotus spp. Saudi Journal of Biological Science. 26: 633-646.
  3. Cheng, Z., Wu, Q., Huang, J.B., Hu, C.G., Wang, Z.L. (2013). Effects of carbon sources, nitrogen sources and minerals on mycelia growth of Cryphonectria parasitica. African Journal of Agricultural Research. 8: 4390-5.
  4. Choi, I.Y., Joung, G.T., Ryu, J., Choi, J.S., Choi, Y.G. (2003). Physiological characteristics of green mold (Trichoderma spp.) isolated from oyster mushroom (Pleurotus spp.). Mycobiology. 31: 139-44.
  5. Chorváthová, V., Bobek, P., Ginterand, E., Klavanová, J. (1993). Effect of the oyster fungus on glycemia and cholesterolemia in rats with insulin-dependent diabetes. Physiological Research. 42: 175-9.
  6. Dehariya, P., Vyas, D., Kashaw, S.K. (2013). Mushroom nutraceuticals on different substrates. International Journal of Pharmacy and Pharmaceutical Sciences. 5: 88-90.
  7. Dung, L.B. (2003). Mushrooms in Tay Nguyen. Ha Noi: Science and Technique.
  8. Egwim, E.C., Ellen, R.C., Egwuche, R.U. (2011). Proximate composition, phytochemical screening and antioxidant activity of ten selected edible mushrooms. American Journal of Food and Nutrition. 1(2): 89-94.
  9. Eira, A.F. (2003). Cultivo do Cogumelo Medicinal. Editora AprendaFa´cil, Vic¸osa (in Portuguese). 
  10. Fu, R., Yin, C., Liu, Y., Ding, L., Zhu, J., Zheng, A., Li, P. (2013). The influence of nutrient and environmental factors on mycelium growth and conidium of false smut Villosiclava virens. African Journal of Microbial Research. 7: 825-33.
  11. Hoa, H.T., Wang, C. (2015). The effects of temperature and nutritional conditions on mycelium growth of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology. 43: 14-23.
  12. ICAR-Agricultural Technology Aplication Research Institute (ATARI) Indian Council of Agricultural Research JNKVV, Jabalpur- 482004 (M.P.). Training Manual on Tropical Mushroom Production and Value Addition. Pp 28.
  13. Janardhanan, K., Vadivel, V. and Pugalenti (2003). Biodiversity in Indian under Exploited/tribal Pulses. In: Improvement Strategies for Leguminosae Biotechnology, [P.K. Jaiwal and R.P. Singh (Eds.)]. Kluwer Academic Publishers. Printed in Great Britain. pp: 353-405.
  14. Kashangura, C. (2008). Optimisation of the growth conditions and genetic characterization of Pleurotus species [dissertation]. Harare: Department of Biological Sciences, Faculty of Science, University of Zimbabwe.
  15. Kües, U., Liu Y.  (2000). Fruiting body production in Basidiomycetes. Applied Microbiology and Biotechnology. 54: 141-52.
  16. Kuforiji, O.O., Fasidi, I.O. (2007). Factor affecting the yield of fruit body and sclerotia in Pleurotus tuber-regium. Advance Food Science. 29: 211-215. 
  17. Kurtzman, R.H., Zadražil, F. (1989). Physiological and Taxonomical Considerations for Cultivation of Pleurotus Mushrooms. In: Tropical Mushrooms Biological Nature and Cultivation Methods. [Chang, S.T., Quimio, T.H., editors]. Hong Kong: Chinese University Press. p. 299-348.
  18. Lee, J.Y. (1993). Coloured Korean Mushrooms. Seoul: Academy Press.
  19. Li, L., Ng, T.B., Song, M., Yuan, F., Liu, Z.K., Wang, C.L., Jiang Y., Fu, M., Liu, F.A. (2007). Polysaccharide-peptide complex from abalone mushroom (Pleurotusa balonus) fruiting bodies increases activities and gene expression of antioxidant enzymes and reduces lipid peroxidation in senescence-accelerated mice. Applied Microbiology and Biotechnology. 75: 863-869.
  20. Mane, V.P., Patil, S.S., Syed, A.A., Baig, M.M. (2007). Bioconversion of low quality lignocellulosic agricultural waste into edible protein by Pleurotussajor-caju (Fr.) Singer. Journal of Zhejiang University Science B. 8: 745-751.
  21. Mao, X.B., Eksriwong, T., Chauvatcharin, S., Zhong J.J. (2005). Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris. Process Biochemistry. 40: 1667-1672.
  22. Miles, P.G., Chang, S.T. (1997). Mushroom Biology: Concise Basics and Current Developments. Singapore: World Scientific, p. 40-4.
  23. Mshandete, A.M., Mgonja, J.R. (2009). Submerged liquid fermentation of some Tanzanian Basidiomycetes for the production of mycelial biomass, exopolysaccharides and mycelium protein using wastes peels media. Journal of Agriculture Biological Science. 4: 1-13.
  24. Munsur, M.A., Miah, A., Rahman, M.H., Rahman, M.M., Yahia, A.S. (2012). Effect of varieties and media on mycelial growth and substrate on spawn production of oyster mushroom. Bangladesh Research Publications Journal. 7: 361-366.
  25. Neelam, S., Chennupati, S., Singh, S. (2013). Comparative studies on growth parameters and physio-chemical analysis of Pleurotus ostreatus and Pleurotus florida. Asian Journal of Plant Science Research. 3: 163-169.
  26. Purkayastha, R.P.A. (1985). Chandra, Manual of Indian Edible Mushrooms, (Today and Tomorrow’s Publication, New Delhi). 267-270.
  27. Pushpa, H., Purushothama, K.B. (2010). Nutritional analysis of wild and cultivated edible medicinal mushrooms. World Journal of Dairy Food Science. 5(2): 140-144.
  28. Sánchez, C. (2010). Cultivation of Pleurotus ostreatus and other edible mushrooms. Applied Microbiology and Biotechnology. 85: 1321-1337.
  29. Tinoco, R., Pickard, M.A., Vazquet-Duhalt, R. (2011). Kinetic differences of purified laccases from six Pleurotus ostreatus strains. Letter in Applied Microbiology. 32: 331-335.
  30. Tsujiyama, S., Ueno, H. (2013). Performance of wood-rotting fungibased enzymes on enzymic saccharification of rice straw. Journal of Science Food Agriculture. 93: 2841-2848.
  31. Wasser, S.P., Weis, A.L. (1999). Therapeutic effects of substances occurring in higher Basidiomycetes mushrooms: A modern perspective. Critical Review in Immunology. 19: 65-96.

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