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Current IssueEditorial BoardOnline First ArticlesArchive

Short Communication

volume 36 issue 3 (september 2021) : 270-272,   Doi: 10.18805/BKAP327

Advanced Strategies in Bulked Segregant Analysis and its Applications

H
Harshavardan J. Hilli
1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141 027, Punjab, India.

  • Submitted07-07-2021|

  • Accepted13-07-2021|

  • First Online 28-09-2021|

  • doi 10.18805/BKAP327

Cite article:- Hilli J. Harshavardan (2021). Advanced Strategies in Bulked Segregant Analysis and its Applications. Bhartiya Krishi Anusandhan Patrika. 36(3): 270-272. doi: 10.18805/BKAP327.

ABSTRACT

Bulked segregant analysis (BSA) is a technique used to identify genetic markers associated with a mutant phenotype and is a quick method for identifying markers in particular genome regions. The paper focussed on Advanced methods which escape the requirement of genotyping all the individuals of the mapping population and generation of high-density linkage maps for mapping of the gene for the trait of interest. With the emergence of re-sequencing techniques, quick mapping of genes has become possible with reduced time and cost by using advanced methodologies like MutMap, MutMap+, MutMap-Gap, QTL-Seq, RNAseq BSA, NGS BSA and QTG seq. The procedure for various advanced BSA strategies has been described. 

REFERENCES


  1. Abe, A., Kosugi, S., Yoshida, K., Natsume, S., Takagi, H., Kanzaki, H., Matsumura, H., Yoshida, K., Mitsuoka, C., Tamiru, M. and Innan, H., (2012). Genome sequencing reveals agronomically important loci in rice using MutMap. Nature Biotechnology. 30(2): 174.

  2. Fekih, R., Takagi, H., Tamiru, M., Abe, A., Natsume, S., Yaegashi, H., Sharma, S., Sharma, S., Kanzaki, H., Matsumura, H. and Saitoh, H., (2013). MutMap+: Genetic mapping and mutant identification without crossing in rice. PloS One. 8(7): 180.

  3. Kishore, U., Kumar, K., Sevanthi, A.M. and Gaikwad, K., (2018). MutMap: A versatile tool for identification of mutant loci and mapping of genes. Indian Journal of Plant Physiology. 23(4): 612-621.

  4. Pandey, M.K., Khan, A.W., Singh, V.K., Vishwakarma, M.K., Shasidhar, Y., Kumar, V., Garg, V., Bhat, R.S., Chitikineni, A., Janila, P. and Guo, B. (2017). QTL seq approach identified genomic regions and diagnostic markers for rust and late leaf spot resistance in groundnut (Arachis hypogaea L.). Plant Biotechnology Journal. 15(8): 927-941.

  5. Takagi, H., Tamiru, M., Abe, A., Yoshida, K., Uemura, A., Yaegashi, H., Obara, T., Oikawa, K., Utsushi, H., Kanzaki, E. and Mitsuoka, C., (2015). MutMap accelerates breeding of a salt-tolerant rice cultivar. Nature Biotechnology. 33(5): 445-449.

  6. Zhang, H., Wang, X., Pan, Q., Li, P., Liu, Y., Lu, X., Zhong, W., Li, M., Han, L., Li, J. and Wang, P., (2019). QTG-Seq accelerates QTL fine mapping through QTL partitioning and whole- genome sequencing of bulked segregant samples. Molecular Plant. 12(3): 426-437.
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    Short Communication

    volume 36 issue 3 (september 2021) : 270-272,   Doi: 10.18805/BKAP327

    Advanced Strategies in Bulked Segregant Analysis and its Applications

    H
    Harshavardan J. Hilli
    1Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141 027, Punjab, India.

    • Submitted07-07-2021|

    • Accepted13-07-2021|

    • First Online 28-09-2021|

    • doi 10.18805/BKAP327

    Cite article:- Hilli J. Harshavardan (2021). Advanced Strategies in Bulked Segregant Analysis and its Applications. Bhartiya Krishi Anusandhan Patrika. 36(3): 270-272. doi: 10.18805/BKAP327.

    ABSTRACT

    Bulked segregant analysis (BSA) is a technique used to identify genetic markers associated with a mutant phenotype and is a quick method for identifying markers in particular genome regions. The paper focussed on Advanced methods which escape the requirement of genotyping all the individuals of the mapping population and generation of high-density linkage maps for mapping of the gene for the trait of interest. With the emergence of re-sequencing techniques, quick mapping of genes has become possible with reduced time and cost by using advanced methodologies like MutMap, MutMap+, MutMap-Gap, QTL-Seq, RNAseq BSA, NGS BSA and QTG seq. The procedure for various advanced BSA strategies has been described. 

    REFERENCES


    1. Abe, A., Kosugi, S., Yoshida, K., Natsume, S., Takagi, H., Kanzaki, H., Matsumura, H., Yoshida, K., Mitsuoka, C., Tamiru, M. and Innan, H., (2012). Genome sequencing reveals agronomically important loci in rice using MutMap. Nature Biotechnology. 30(2): 174.

    2. Fekih, R., Takagi, H., Tamiru, M., Abe, A., Natsume, S., Yaegashi, H., Sharma, S., Sharma, S., Kanzaki, H., Matsumura, H. and Saitoh, H., (2013). MutMap+: Genetic mapping and mutant identification without crossing in rice. PloS One. 8(7): 180.

    3. Kishore, U., Kumar, K., Sevanthi, A.M. and Gaikwad, K., (2018). MutMap: A versatile tool for identification of mutant loci and mapping of genes. Indian Journal of Plant Physiology. 23(4): 612-621.

    4. Pandey, M.K., Khan, A.W., Singh, V.K., Vishwakarma, M.K., Shasidhar, Y., Kumar, V., Garg, V., Bhat, R.S., Chitikineni, A., Janila, P. and Guo, B. (2017). QTL seq approach identified genomic regions and diagnostic markers for rust and late leaf spot resistance in groundnut (Arachis hypogaea L.). Plant Biotechnology Journal. 15(8): 927-941.

    5. Takagi, H., Tamiru, M., Abe, A., Yoshida, K., Uemura, A., Yaegashi, H., Obara, T., Oikawa, K., Utsushi, H., Kanzaki, E. and Mitsuoka, C., (2015). MutMap accelerates breeding of a salt-tolerant rice cultivar. Nature Biotechnology. 33(5): 445-449.

    6. Zhang, H., Wang, X., Pan, Q., Li, P., Liu, Y., Lu, X., Zhong, W., Li, M., Han, L., Li, J. and Wang, P., (2019). QTG-Seq accelerates QTL fine mapping through QTL partitioning and whole- genome sequencing of bulked segregant samples. Molecular Plant. 12(3): 426-437.
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