Short Communication

Bhartiya Krishi Anusandhan Patrika, volume 36 issue 3 (september 2021) : 270-272

Advanced Strategies in Bulked Segregant Analysis and its Applications

Harshavardan J. Hilli

Affiliations

¹Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141 027, Punjab, India.

Submitted07-07-2021|
Accepted13-07-2021|
First Online 28-09-2021|
doi 10.18805/BKAP327

Cite article:- Hilli J. Harshavardan (2021). Advanced Strategies in Bulked Segregant Analysis and its Applications. Bhartiya Krishi Anusandhan Patrika. 36(3): 270-272. doi: 10.18805/BKAP327.

ABSTRACT

Bulked segregant analysis (BSA) is a technique used to identify genetic markers associated with a mutant phenotype and is a quick method for identifying markers in particular genome regions. The paper focussed on Advanced methods which escape the requirement of genotyping all the individuals of the mapping population and generation of high-density linkage maps for mapping of the gene for the trait of interest. With the emergence of re-sequencing techniques, quick mapping of genes has become possible with reduced time and cost by using advanced methodologies like MutMap, MutMap+, MutMap-Gap, QTL-Seq, RNAseq BSA, NGS BSA and QTG seq. The procedure for various advanced BSA strategies has been described.

KEYWORDS

REFERENCES

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Zhang, H., Wang, X., Pan, Q., Li, P., Liu, Y., Lu, X., Zhong, W., Li, M., Han, L., Li, J. and Wang, P., (2019). QTG-Seq accelerates QTL fine mapping through QTL partitioning and whole- genome sequencing of bulked segregant samples. Molecular Plant. 12(3): 426-437.

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Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Short Communication

Advanced Strategies in Bulked Segregant Analysis and its Applications

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