Loading...

Advanced Strategies in Bulked Segregant Analysis and its Applications

DOI: 10.18805/BKAP327    | Article Id: BKAP327 | Page : 270-272
Citation :- Advanced Strategies in Bulked Segregant Analysis and its Applications.Bhartiya Krishi Anusandhan Patrika.2021.(36):270-272
Harshavardan J. Hilli harshajh1995@gmail.com
Address : Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana-141 027, Punjab, India.
Submitted Date : 7-07-2021
Accepted Date : 13-07-2021

Abstract

Bulked segregant analysis (BSA) is a technique used to identify genetic markers associated with a mutant phenotype and is a quick method for identifying markers in particular genome regions. The paper focussed on Advanced methods which escape the requirement of genotyping all the individuals of the mapping population and generation of high-density linkage maps for mapping of the gene for the trait of interest. With the emergence of re-sequencing techniques, quick mapping of genes has become possible with reduced time and cost by using advanced methodologies like MutMap, MutMap+, MutMap-Gap, QTL-Seq, RNAseq BSA, NGS BSA and QTG seq. The procedure for various advanced BSA strategies has been described. 

Keywords

BSA Mutmap Mutmap+ Mutmap-gap QTG seq QTL seq

References

  1. Abe, A., Kosugi, S., Yoshida, K., Natsume, S., Takagi, H., Kanzaki, H., Matsumura, H., Yoshida, K., Mitsuoka, C., Tamiru, M. and Innan, H., (2012). Genome sequencing reveals agronomically important loci in rice using MutMap. Nature Biotechnology. 30(2): 174.
  2. Fekih, R., Takagi, H., Tamiru, M., Abe, A., Natsume, S., Yaegashi, H., Sharma, S., Sharma, S., Kanzaki, H., Matsumura, H. and Saitoh, H., (2013). MutMap+: Genetic mapping and mutant identification without crossing in rice. PloS One. 8(7): 180.
  3. Kishore, U., Kumar, K., Sevanthi, A.M. and Gaikwad, K., (2018). MutMap: A versatile tool for identification of mutant loci and mapping of genes. Indian Journal of Plant Physiology. 23(4): 612-621.
  4. Pandey, M.K., Khan, A.W., Singh, V.K., Vishwakarma, M.K., Shasidhar, Y., Kumar, V., Garg, V., Bhat, R.S., Chitikineni, A., Janila, P. and Guo, B. (2017). QTL seq approach identified genomic regions and diagnostic markers for rust and late leaf spot resistance in groundnut (Arachis hypogaea L.). Plant Biotechnology Journal. 15(8): 927-941.
  5. Takagi, H., Tamiru, M., Abe, A., Yoshida, K., Uemura, A., Yaegashi, H., Obara, T., Oikawa, K., Utsushi, H., Kanzaki, E. and Mitsuoka, C., (2015). MutMap accelerates breeding of a salt-tolerant rice cultivar. Nature Biotechnology. 33(5): 445-449.
  6. Zhang, H., Wang, X., Pan, Q., Li, P., Liu, Y., Lu, X., Zhong, W., Li, M., Han, L., Li, J. and Wang, P., (2019). QTG-Seq accelerates QTL fine mapping through QTL partitioning and whole- genome sequencing of bulked segregant samples. Molecular Plant. 12(3): 426-437.

Global Footprints