Utilization of fish scales waste for extracting chitin and chitosan 

Tarun Kumar Varun, Swaraj Senani, Narender Kumar, Ritika Gupta, Mayank Gautam, Mokshata Gupta
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1<p>ICAR-National Institute of Animal Nutrition and Physiology, Bangalore (Karnataka) &nbsp;India&nbsp;</p>
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Cite article:- Varun Kumar Tarun, Senani Swaraj, Kumar Narender, Gupta Ritika, Gautam Mayank, Gupta Mokshata (NaN). Utilization of fish scales waste for extractingchitin and chitosan . Bhartiya Krishi Anusandhan Patrika. 32(2): 139-142. doi: undefined.

In the present study, fish scales waste was processed to produce chitin and chitosan. Chemical extraction was followed for chitin and chitosan production. In this method, deproteination and demineralization was done by using NaOH and HCl, respectively. After these two steps formed compound was chitin which was further processed to form chitosan following deacetylation step. Compositional analysis of fish scale waste was done by standard methods which reveal that protein content was high. Mineral composition analyzed by Inductively Coupled Plasma Optical Emission (ICP-OES) confirms the presence of high amount of macro minerals (%) and micro minerals (ppm) such as calcium, phosphorous and copper, iron i.e. 13.9±0.21, 5.77±0.05 and 129.30±8.05, 83.39±15.48, respectively. Chitin and chitosan yield (%) from fish scale waste was found to be 1.1 ± 0.004 and 0.85 ± 0.01 which shows that fish scale waste is a good source of chitin and chitosan.

  1. Anonymous (2014). Hand Book of Fisheries Statistics, DAHDF, Ministry of Agriculture, Government of India, New Delhi. 

  2. Abdulkarim, A., Isa, M.T., Abdulsalam, S., Muhammad, A.J. and Ameh, A.O. (2013). Extraction and characterisation of chitin and chitosan from mussel shell. Civ. Environ. Res. 3(2): 108-114.

  3. Anraku, M., Fujii, T., Furutani, N., Kadowaki, D., Maruyama, T., Otagiri, M., Gebicki, J.M. and Tomida, H. (2009). Antioxidant effects of a dietary supplement: Reduction of indices of oxidative stress in normal subjects by water-soluble chitosan. Food and Chemical Toxicology. 47(1):104-109.

  4. AOAC. (1990). Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Washington, DC, USA.

  5. Benhabiles, M.S., Salah, R., Lounici, H., Drouiche, N.,Goosen, M.F.A. and Mameri, N. (2012). Antibacterial activity of chitin, chitosan and its oligomers prepared from shrimp shell waste. Food hydr. 29(1): 48-56.

  6. Chen, K.J., Chiu, Y.L., Chen, Y.M., Ho, Y.C. and Sung, H.W. (2011). Intracellularly monitoring/imaging the release of doxorubicin from pH-responsive nanoparticles using Förster resonance energy transfer. Biomaterials. 32: 2586-2592.

  7. Cho, Y.I., No, H.K. and Meyers, S.P. (1998). Physicochemical characteristics and functional properties of various commercial chitin and chitosan products. J. Agric. Food Chem. 46: 3839– 3843.

  8. Costa, E.M., Silva, S., Pina, C., Tavaria, F.K. and Pintado, M.M. (2012). Evaluation and insights into chitosan anti microbial activity against anaerobic oral pathogens. Anaerobe. 18(3): 305-309.

  9. Fernandes, J.C., Tavaria, F.K., Soares, J.C., Ramos, O.S., Monterio, M.J., Pintado, M.E. and Malcata, F.X. (2008). Antimicrobial effects of chitosans and chitooligosaccharides, upon Staphylococcus aureus and Escherichia coli, in food model systems. Food Microbiol. 25(7): 922-928.

  10. Ghaly, A.E., Ramakrishnan, V.V., Brooks, M.S., Budge, S.M. and Dave, D. (2013). Fish processing wastes as a potential source of proteins, amino acids and oils: A critical review. J Microb Biochem Technol. 5: 107-129. 

  11. Hussain, I., Singh, T. and Chittenden, C. (2012) Preparation of chitosan oligomers and characterization: Their antifungal activities and decay resistance. Holzforschung. 66(1): 119-125.

  12. Mathur, N.K. and Narang, C.K. (1990). Chitin and chitosan versatile polysaccharides from marine animals. J Chem Educ. 67:938.

  13. Muslim, T., Rahman, M.H., Begum, H.A. and Rahman, M.A. (2013). Chitosan and carboxymethyl chitosan from fish scales of Labeo rohita. Dhaka University Journal of Science. 61(1):145-148.

  14. Varun, T.K., Senani, S., Jayapal, N., Chikkerur, J., Roy, S., Tekulapally, V.B., Gautam, M. and Kumar, N. (2017). Extraction of chitosan and its oligomers from shrimp shell waste, their characterization and antimicrobial effect. Veterinary World. 10(2): 170-175.

  15. Mahboob, S. (2015). Isolation and characterization of collagen from fish waste material- skin, scales and fins of Catla catla and Cirrhinusmrigala. J Food Sci Technol. 52(7):4296–4305.


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