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Research Article

Bhartiya Krishi Anusandhan Patrika, volume 36 issue 3 (september 2021) : 215-223

​Selecting the Best Combination of Adjuvant and the Delivery Vehicle for Optimizing the Th1 and Th2 Biased Responses of NDV Live Vaccine in Indigenous Aseel Chicken

Manmeet Kaur, C.S. Mukhopadhyay, Ramneek, R.S. Sethi, Dipak Deka, P.P. Dubey
1College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ferozepur Road, Ludhiana-141 001, Punjab, India.

  • Submitted17-06-2021|

  • Accepted17-08-2021|

  • First Online 26-08-2021|

  • doi 10.18805/BKAP304

Cite article:- Kaur Manmeet, Mukhopadhyay C.S., Ramneek, Sethi R.S., Deka Dipak, Dubey P.P. (2021). ​Selecting the Best Combination of Adjuvant and the Delivery Vehicle for Optimizing the Th1 and Th2 Biased Responses of NDV Live Vaccine in Indigenous Aseel Chicken. Bhartiya Krishi Anusandhan Patrika. 36(3): 215-223. doi: 10.18805/BKAP304.

ABSTRACT

Background: This study aimed at the evaluation of the efficacy of TLR ligands (viz. Poly I:C, Lipopolysaccharide and CpG:ODN) as vaccine adjuvants in combination with a suitable carrier (oil-in-water emulsion, normal saline solution, or alum) by studying the expression profile of cytokines belonging to Th1 and Th2 biased immune systems. 
Methods: Whole blood was collected from 108 birds that were divided into 9 different experimental groups: 3 TLR ligands each with 3 delivery vehicles. Th1 (IFN-gamma) and Th2 (IL4) biased cytokines were detected by qPCR followed by ELISA. Both the results showed the highest level of IL4 (Th1 biased) with LPS and the highest level of IFNg (Th1 biased) with CpG-ODN. Uneven TLR expression was detected from day 3 onwards till day 21 but ligands and delivery vehicles administered along with NDV vaccine did not show significant influence on the immune response.
Result: It is evident that the TLR-3, 4 and 9 expressions increase following exposure to the respective ligands. Hence, TLR ligands can be used as reliable adjuvants in NDV vaccine. The expression of the Th2 biased cytokine IL4 mRNA and Th1 biased cytokine Interferon-gamma was found to be the highest on day 3 in the LPS+NSS group and on day 21 in CpG-ODN+Alum group, respectively. The findings of the real-time PCR were supported to some by the ELISA. 

REFERENCES


  1. Abdul-Careem, M.F., Hunter, D.B., Lambourne, M.D., Barta, J. and Sharif, S. (2007). Ontogeny of cytokine gene expression in the chicken spleen. Poultry Science. 86(7): 1351-1355. 

  2. Abu-Baker, N. and Hussein, M. (2016). Synthesis, characterization and immunological properties of LPS-based vaccines composed of O-Polysaccharides conjugated with recombinant  exoprotein a from Pseudomonas aeruginosa. Advances in Microbiology. 6: 332-342.

  3. Aini, H.H., Omar, A.R., Hair-Bejo, M., Aini, I. (2008). Comparison of Sybr Green I, ELISA and conventional agarose gel- based PCR in the detection of infectious bursal disease virus. Microbiol Res. 163(5): 556-63. doi: 10.1016/j.micres. 2006.08.003.

  4. Alexander, D.J. (1997). Newcastle disease and other avian Paramyxoviridae infections. In: Diseases of Poultry, [Calnek, B.W., Barnes, H.J., Beard, C.W., McDougald, L.R. and Saif, Y.M. (Eds.)] 10th Edn. Ames: Iowa State University Press. pp 541-70.

  5. Alexopoulou, L., Holt, A.C., Medzhitov, R. and Flavell, R.A. (2001). Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature. 413(6857): 732–738. 

  6. Beutler, B., Eidenschenk, C., Crozat, K., et al. (2007) Genetic analysis of resistance to viral infection. Nat. Rev. Immunol. 7: 753-766. 

  7. Defang, G.N., Martin, N.J., Burgess, T.H., Millar, E.V., Pecenka, L.A., Danko, J.R., Arnold, J.C., Kochel, T.J. and Luke, T.C. (2012). Comparative analysis of hemagglutination inhibition titers generated using temporally matched serum and plasma samples. PLoS ONE. 7(12): e48229. 

  8. Elston, T., Rodan, H., Flemming, D., Ford, R. B., Hustead, D. R., Richards, J. R., Rosen, D. K., Scherk-Nixon, M. A. and Scott, P. W. (1998). Report of the American Association of Feline Practitioners and Academy of Feline Medicine Advisory Panel on Feline Vaccines. Journal of American Veterinary Medical Association. 212: 227-241.

  9. Giambrone J.J. (1985). Laboratory evaluation of Newcastle disease vaccination programs for broiler chickens. Avian Dis. 29(2): 479-87.

  10. Han, Y., Bo, Z.J., Xu, M.Y., Sun, N. and Liu, D.H. (2014). The Protective Role of TLR3 and TLR9 Ligands in Human Pharyngeal Epithelial Cells Infected with Influenza A Virus. Korean Journal of Physiology and Pharmacology: Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology. 18(3): 225-231.

  11. Hsieh, C.S., Macatonia, S.E., Tripp, C.S., Wolf, S.F., O’Garra, A. and Murphy, K.M. (1993). Development of Th1 CD4+ T cells through IL-12 produced by Listeria-induced macrophages. Science (New York, N.Y.). 260(5107): 547-549.

  12. Khalifeh, M.S., Amawi, M.M., Abu-Basha, E.A. and Yonis, I.B. (2009). Assessment of humoral and cellular-mediated immune response in chickens treated with tilmicosin, florfenicol, or enrofloxacin at the time of Newcastle disease vaccination. Poultry Science. 88(10): 2118-2124. 

  13. Lee, J.M., Kim, J.G., Kim, T.H., Lee, D.S., Kim, J.H., Cho, S.K., Riu, K.Z., Lee, D.S. and Lee, S.H. (2010). Nonactin hinders intracellular glycosylation in virus-infected baby hamster kidney cells. Molecular Medicine Reports. 3(1): 115-119. 

  14. Livak, K.J. and Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 25(4): 402-8. doi: 10.1006/meth.2001.1262. 

  15. Ranjith-Kumar, C.T., Duffy, K.E., Jordan, J.L., Eaton-Bassiri, A., Vaughan, R., Hoose, S.A., Lamb, R.J., Sarisky, R.T. and Kao, C.C. (2008). Single-stranded oligonucleotides can inhibit cytokine production induced by human toll-like receptor 3. Molecular and Cellular Biology. 28(14): 4507- 4519.

  16. Smbrook, J. and Russell, D.W. (2012). Molecular Cloning: A laboratory manual. Edition 4. ISBN 978-1-936113-42-2.

  17. Smith, H.W. (1956). The use of thelive vaccine in experimental Salmonella gallinarum infection in chickens with observations on their interference effect. The Journal of Hygiene. 54(3): 419-432.

  18. Sokol, H., Pigneur, B., Watterlot, L., Lakhdari, O., Bermúdez-Humarán, L.G., Gratadoux, J.J., Blugeon, S., Bridonneau, C. et al (2008). Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. Proceedings of the National Academy of Sciences of the United States of America.  105(43): 16731-16736.

  19. Su, J., Li, S., Hu, X., Yu, X., Wang, Y. et al. (2011). Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus. PLoS One. 6: e18106.

  20. Sundick, R.S. and Gill-Dixon, C. (1997). A cloned chicken lymphokine homologous to both mammalian IL-2 and IL-15. Journal of Immunology (Baltimore, Md. : 1950). 159(2): 720-725.

  21. Takeda, K., Kaisho, T. and Akira, S. (2003). Toll-like receptors. Annual Review of Immunology. 21: 335-376.
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