Bhartiya Krishi Anusandhan Patrika, volume 37 issue 2 (june 2022) : 151-156

The Impact of Emblica officinalis on Altered Biochemical Markers and Oxidative Stress Indices After Sub-acute Enrofloxacin Treatment in Albino Rats

Prateek Mishra, Vidhi Gautam, R.K. Sharma, Sachin Jain, Anushri Tiwari, Vijay Gond
1Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.
  • Submitted06-03-2022|

  • Accepted03-06-2022|

  • First Online 20-06-2022|

  • doi 10.18805/B-4896

Cite article:- Mishra Prateek, Gautam Vidhi, Sharma R.K., Jain Sachin, Tiwari Anushri, Gond Vijay (2022). The Impact of Emblica officinalis on Altered Biochemical Markers and Oxidative Stress Indices After Sub-acute Enrofloxacin Treatment in Albino Rats. Bhartiya Krishi Anusandhan Patrika. 37(2): 151-156. doi: 10.18805/B-4896.
Background: The current study was conducted at the Department of Veterinary Pharmacology and Toxicology to assess the impact of Emblica officinalis on oxidative stress produced by sub-acute enrofloxacin exposure in albino rats.
Methods: Albino rats weighing 200-300 gram were given enrofloxacin and Emblica officinalis orally through gavage needle and blood samples were taken via capillary tube on the 28th day of the experiment and analysed using laboratory procedures.
Result: Sub-acute treatment of Enrofloxacin at doses of 5 mg/kg b.wt. and 10 mg/kg b.wt. generated oxidative stress in rats, as evidenced by substantial changes in oxidative parameters and affected liver and kidney functions, as seen by changes in biochemical markers of liver and kidney function. Emblica officinalis aqueous extract (200 mg/kg b. wt.) effectively reduces oxidative stress and changes in hepatic and renal function caused by enrofloxacin.

  1. Aebi, H.E. (1983). Catalase, In: Methods of Enzymatic Analysis, [H.U. Bergmeyer, J. Berg- meyer and M. GraBI, Eds.], 3rd Edition, Verlag Chemie, Weinheim, b. pp. 273-286.   

  2. Alia, M., Mateos, R., Ramos, S., Lecumberri, E., Bravo, L. and Goya L. (2006). Influence of quercetin on growth and antioxidant defense system of a human hepatoma cell line (HepG2). Europian Journal of Nutrure. 45: 19-28.

  3. Anila, L. and Vijayalakshmi, N.R. (2003). Antioxidant action of flavonoids from Mangifera indica and Emblica officinalis in hypercholesterolemic rats. Journal of Food Chemistry. 83(4): 569-574.

  4. Archar, R.K. and Riley, J. (1981). Standardized method of bleeding rats. Laboratory Animals. 15: 25-28.          

  5. Bast, A., Maenon, G.R. and Doelman, G.J. (1991). Antioxidant activity of plants herb. American Journal of Medicine. 91: 251-254.

  6. Bhandari, P.R. and Kamdod, M.A. (2012). Emblica officinalis (Amla): A review of potential therapeutic applications. International Journal of Green Pharmacy. 6(4): 257-269.

  7. Bhattacharya, A., Chattergee, A., Ghosal, S. and Bhattacharya, S.K. (1999). Antioxidant activity of active tannoids principles of Emblica officinalis (amla). Indian Journal of Experimental Biology. 37: 676 80.

  8. Bhattacharya, S.K., Bhattacharya, A., Sairam, K. and Ghosal, S. (2002). Effect of bioactive tannoids principles of Emblica officinalis on ischemia reperfusion- induced oxidative stress in rat heart. Journal of Phytomedicine. 9: 171-174.

  9. Carreras, I., Castellari, M., Valero, A., Antonio, J. and Sarraga, C. (2005). Influence of enrofloxacin administration on the proteolytic and antioxidant enzyme activities of raw and cooked turkey products. Journal of Food Science and Agriculture. 85: 2407-2412.

  10. Davoren, P. and Mainstone, K. (1993). Norfloxacin induced hepatitis. Medical Journal of Australia. 159(6): 423-426.

  11. Elbe, H., Dogan, Z., Taslidere, E., Cetin, A. and Turkoz, Y. (2015). Beneficial effect of quercetin on renal injury and oxidative stress induced by ciprofloxacin in rats. Journal of Human and Experimental Toxicology. 35(3): 276-281.

  12. Elkholy, H., Elkomy, A., Awaidat, S. and Elmajdoub, A.A. (2009). Tissue and egg residues and adverse effect of two oral enrofloxacin preparations; Baytril and Enrotryl. Journal of Global Veterinari. 3(5): 363-368. 

  13. Gulati, R.K., Agarwal, S. and Sharma, S.S. (1995). Hepatoprotective studies on Phyllanthus emblica Linn and quercetin. Indian Journal of Experimental Biolog. 33(4): 261-268. 

  14. Guo, J., Zhang, D. and Imal, M. (2018). Advance in the research on the mechanism of tonifying kidney herbs that protect the articular cartilage. Journal of Traditional Chinese Medicine. 28: 2515-2518. 

  15. Gurbay, A., Gonthier, B., Singorini, N., Barret, L., Favier, A. and Hincal, F. (2006). Ciprofloxacin induced DNA damage in primary culture of rat astrocyte and protection by Vitamin E. Journal of Neurotoxicoloy. 27(1): 6-10.

  16. Habig, W.H., Pabst, M.J., Jakoby, W.B. (1974) Glutathione-S- transferase: The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistr. 249: 7130-7139.

  17. Ibrahim, I.G. and Yarsan, E. (2011). Enrofloxacin drug induced reactive oxygen species. Journal of Research Opinion in Animal and Veterinary Sciences. 1(8): 489-491.

  18. Jiang, L., Wang, W. and Luo, L. (2014). Blood concentration and histological toxicology of different dosages of florfenicol in GIFT Nile tilapia. Journal of Shanghai Ocean University. 23: 90-94.

  19. Khan, A.M. and Rampal, S. (2014). Effect of repeated oral administration of levofloxacin, enrofloxacin and meloxicam on antioxidant parameters and lipid peroxidation in rabbits. Human and Experimental Toxicology. 36(1): 216-218.

  20. Khopde, S.M., Priyadarsini, K.I., Mohan, M. and Banabaliker, M.M. (2001). Chareccterising the antioxidant activity of amla (Phyllanthus Emblica) extract. Journal of Current Science. 8(1): 185-190.

  21. Kock, M.D., Jessup, D., Clark, R. and Frantic, C. (1987). Effects of capture on biological parameters in free-ranging bighorn sheep (Ovis canadensis): Evaluation of drop-net, drive- net, chemical immobilization and the net-gun. Journal of Wildlife Diseases. 23: 641-651.

  22. Kock, M.D., Jessup, D., Clark, R. and Frantic, C. (1987). Effects of capture on biological parameters in free-ranging bighorn sheep (Ovis canadensis): Evaluation of drop-net, drive- net, chemical immobilization and the net-gun. Journal of Wildlife Diseases. 23: 641-651.

  23. Krishnaveni, M., Swathi, A. and Mirunalini, S. (2010). Therapeutic potential of Phyllanthus emblica (amla): The ayurvedic wonder. Journal of Basic Clinical Physiology and Pharmacology. 21: 93-105.

  24. Marklund, S. and Marklund, G. (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 47: 469-474

  25. Moustafa, A., Baset, I., Mashad, A. and Tantawy, A. (1998). Pathological studies on enrofloxacin and its residues in broiler chickens. Egyptian Journal of Comparative Pathology. 11: 57-66.

  26. Nagraj, R., Deb, U., Bhaskar, S., Prasad, G. and Rao, P.V. (2007). Hepatoprotective efficacy of certain flavonoids against microcystin induced toxicity in mice, Environmental Toxicology. 22: 472-479.

  27. Prins, H.K. and Loos (1969). Biochemical Methods in Red Cell Genetics. Yunis, J.J. Glutathion. Publ. Academic Press. NY. pp.-115-137.

  28. Rajak, S., Banerjee, S.K., Sood, S., Dinda, A.K., Gupta, Y.K., Gupta, S.K. and Maulik, S.K. (2004). Emblica officinalis causes myocardial adaptation andprotects against oxidative stress in ischemic-reperfusion injury in rats. Journal of Phytochemistry. 18: 54-60.

  29. Reddy, V.D., Padmavathi, P., Paramahamsa, M. and Varadacharyulu, N. (2009). Amelioration of alcohol induced oxidative stress by Emblica officinalis (amla) in rats. Indian Journal of Biochemistry and Biophysics. 47: 20 25.

  30. Rehman, S.U. (1984). Lead induced regional lipid peroxidation in brain. Toxicology Letters. 21(3): 333-337.

  31. Sai Ram, M., Neetu, D. and Deepti, P. (2002). Cytoprotective activity of Amla (Emblica officinalis) against chromium (VI) induced oxidative injury in murine macrophages. Journal of Pharmacological Research. 17(4): 430-433. 

  32. Sarban, A., Sezgin, A., Koenig, B., Mithat, Y., Augur, A. and Isikan, E. (2005). Plasma total antioxidant capacity, lipid peroxidation and erythrocyte antioxidant enzyme activities in patients with rheumatoid arthritis and osteoarthritis. Journal of Clinical Biochemistry. 38: 981-986.

  33. Shinwari, M.I. and Khan, P. (1998). Indigenous use of medicinal trees and shrubs of Margalla Hills National Park, Islamabad. Pakistani Journal of Forest. 48: 63-90.

  34. Shukla, D. (2006). Studies on pharmacological action of Boerhaavia diffusa with special reference to its antibacterial activity in rats. M.V.Sc. and A.H. thesis (Pharmacology and Toxicology), Nanaji Deshmukh Veterinary Science University, Jabalpur.

  35. Singh, M., Yadav, R., Yogeshwar, S., Shukla, S. and Sanjay. S. (2014). Efficacy of crude extract of Emblica officinalis (amla) in arsenic-induced oxidative damage and apoptosis in splenocytes of mice. Toxicology Letter. 21(1): 8-17.

  36. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods, 8th Edn., The lowa State College Press, Inc. USA. 950P.

  37. Sureshkumar, V., Sarathchandra, G. and Ramesh, J. (2013). Veterinary pharmacovigilance evaluation on impact of enrofloxacin administration on antioxidant status in broiler chicken. International Journal of Current Microbiological Science. 2: 335-341.

  38. Yazar, E. and Tras, B. (2001). Effect of fluoroquinolone antibiotic on hepatic superoxide dismutase and glutathion peroxidase activites in healthy and expermintelly induced perotenitis mice. Revue De Medecineveterinaire. 15: 200-235.

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