Analysis of E-waste atomic absorption spectroscopy
The trace elements present in the compact discs and batteries in Table (1) showed that the most metals presented in (1 gram) were Cu, Cr, Sr, Br, Ti, Zn and the highest concentrations were Pb, Cd and Ni the last three metals recognized as significant environmental pollutants
(Barnett and Hunter, 1972).
Identification of isolated fungal genera and their ability to pellet formation
Identification of selected isolates fungal colony morphologic investigations of cotton-shaped colonies in PDA plates prepared from selected fungi, Five fungal isolates (
Aspergillus niger,
Aspergillus terreus,
Aspergillus fumigatus,
Penicillium sp. and
Alternaria alternate) were tested to form pellets (Table 2) shows the first and second genera were able to form pellets and after 7 days only
A.
terreus pellets were stay stable after 14 days as showed in (Fig 1). Trace elements biosorption was studded by wet biomass of
Aspergillus terreus pellets, the uptake of trace elements after 48 hrs. of incubation is shown in Fig 2, Electron microscope image (SEM) of
A.
terreus Before preparation. same fungal genera isolated from soil
(Kichu et al., 2020; Temjen et al., 2022).
Effect of pH value on metal biosorption by fungi
In this study, metal ion recovery is strongly influenced by pH. Its changes in the culture media and metal ion adsorption sites have a high effect on cell surface and chemical structure of the metal in water affected by the initial pH on absorption of metal ions, The effect of pH changes on metal ions showed in Fig 3. This study evaluates effect of pH on absorption of metal ions with different values
(Shahabuddin et al., 2022; Narayanasamy et al., 2018). In the experiments, the maximum removal percentage R% of all metals was (89, 91 and 96) observed at pH 5 and R% is significantly (p≤0.05) less than the other pH values.
Effect of different amounts of E-waste powder in solution on the recovery rate in Cadmium, Nickel and lead metals
The effect of different amounts of E-waste powder (0.5,1 and 1.5) g/L on the removal of metals was performed in Fig 4. In all amounts, the effect of E-waste powder in the solution no significant differences (p£0.05) on the removal rate of Cadmium, nickel and lead metals, the effect of metal concentration on the removal of metals by
A.
terreus, the absorption capacity of Cd was about 78-80% and for Ni 969-75% for Pb 90-94%
FT- IR spectra of biosorption by fungal pellets
The bioaccumulation of trace elements is a function of the primary functional groups during the biosorption process by FT-IR spectral analysis. Fig 5 shows the transmittance bands at 646.49,699.90 cm
-1. In biosorption
A.
terreus, transfer bands based on electronic-waste powder density were attained, 3913.22, 2620.04, 1770.37 and 1630.15 cm
-1, which are correlated to C-H-C-H, hydroxylic group and RCO-OH, correspondingly. FT-IR analysis presented that the four main functional groups, which contain amines, hydroxyls, alcohols and amides, play an important role in the bioaccumulation of trace elements during the biosorption experimentations. FT-IR peaks obtained from mycelium fungal biomass isolates were compared with standard interpretation charts for FT-IR peaks.
Isolation identification of fungal genera and its ability to pellet formation
Heavy metal pollution in affluent nations makes them heavily reliant on collecting electronic debris, a conceivable solution is bioremediation and recycling are both possible with microorganisms because of their environmental friendliness and cost-effectiveness. Study results show that filamentous fungi have an excellent ability for heavy metal biosorption under the same conditions
(Parveen et al., 2023; Bahafid et al., 2017). Fungi isolated from polluted soil as previous studies have confirmed that microbial populations can adapt to high levels of heavy metal contamination in heavy metal-polluted environments
(Al-Shammari et al., 2022;
Fan et al., 2018). Because the long-term toxic effects of heavy metal pollution tend to increase the number of native microbial diversity, fungi with a high potential for tolerance to trace elements have been found in these areas, particularly due to their increased adaptability to long-term exposure to pollutants. Some fungi thrive in high (toxic) concentrations of metal ions
(Cardenas Gonzalez et al., 2019).
Acidic pH = 5 was the optimum for removal percent, Therefore, under acidic conditions the cause of competition of H+ ions with heavy metal cations for placement on the surface of fungal hyphae mass, sorption occurs previous studies recorded pH values effect on biosorption of Co (II), 250 mg l
-1 to maximize biosorption, the dehydrated mycelium was incubated at pH 5.5 for one day.
Aspergillus niger,
Penicillium spp. absorbed 93, 77.5 and 70.4% of Co (II),
(Cardenas Gonzalez et al., 2019). Another study found that with
Paecilomyces absorption of cobalt increased with the changing of pH from acidic to neutral. The highest biosorption was verified at pH 7.0 according to the cobalt precipitation
(Li et al., 2014). These findings can be clarified by the fact that there was less competition between hydrogen atoms with positive charge and Co
2+ functional groups.
According to (FT-IR) analysis
A.
terreus used for the bioremediation of trace elements from electronic-waste FT-IR conducted on the trace elements resistant
A.
terreus exposed that 4 main functional groups were noticed on the surface of the fungal hyphae which are amines, hydroxyl, alcohols and amides groups. Bioremediation involves the interaction of trace elements with functional groups on the surface of the fungal cell wall (amines, hydroxyls, alcohols and amides), causing slight shifts or changes in peaks which agreed with previous studies
(Gazem and Nazareth, 2013). FT-IR analysis of the mycelium biomass of fungal species showed that the changes in peak location and concentration were caused by the complexation of functional groups on the surface of the mycelium biomass caused by the hydrolysis of some polysaccharides on the fungal cell wall to shorter saccharides, such as oligosaccharides. A similar finding was reported during the biosorption process of these functional groups with trace elements
(Temjen et al., 2022; Ozsoy et al., 2008), that hydroxyl groups in conjunction with carbonyl groups in mycelial biomass were indicative of carboxylic acid groups. There are amino acids present in the cell wall of the mycelial biomass, which can explain the presence of an amino group and a hydroxyl group with a carbonyl group. The result of this study agreed with results from
Ozsoy et al., (2008), Al-Shammari et al., (2023) All reported that the process of bioaccumulation of trace elements at the fusional groups proceeded by creating a negatively charged surface on the cell wall of fungal cell walls and chitin through the ionization of functional groups, thereby enhancing the binding of heavy metal ions.