Agricultural Science Digest

  • Chief EditorArvind kumar

  • Print ISSN 0253-150X

  • Online ISSN 0976-0547

  • NAAS Rating 4.75

  • SJR 0.156

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Agricultural Science Digest, volume 42 issue 6 (december 2022) : 717-722

Screening of Compounds Secreted by Local Isolates of Phosphate Solubilizing Fungi (PSF) by GC-MS Analysis

N.K. Al-Zubaidi, A.H. Al-Taie
1Department of Plant Protection, College of Agriculture, Wasit University, Iraq.
Cite article:- Al-Zubaidi N.K., Al-Taie A.H. (2022). Screening of Compounds Secreted by Local Isolates of Phosphate Solubilizing Fungi (PSF) by GC-MS Analysis. Agricultural Science Digest. 42(6): 717-722. doi: 10.18805/ag.DF-435.

Background: Microorganisms are an important factor in solubility of nutrition minerals; phosphate solubilizing fungi (PSF) considered one of the main sources of sustainability of agriculture production systems. Some fungi are well known for their natural capability to produce various organic acids, due to the lowering pH in the soil. The study amid to isolate PSF from rhizosphere soil and some plant residue and identify its secrenning compound using GC-MS analysis.
Methods: in laboratory investigation during 2016, isolates of PSF and check their solubilizing activities using selective artificial media PVK and NBRIP in soild and broth form. The secrening of secondary organic compound in broth culture extracted and identify using GC-MS analysis.            
Result: the results inventory six isolates, among these isolates Aspergillus niger AD-A2 and AG-B1 were Distinguished by formed halo-clear zones around their colonies as a result of their phosphate-solubilizing ability in soild PVK and NBRIP media. pH decrese of the final product of PSF isolate in broth medium. Various compounds formed by submerged fermentation of A. niger isolates were 1,3-dioxan-5-ol (96.47%); octanoic acid, 4-chlorobenzyl ester (0.14%); 1,3,5-cycloheptatriene (0.58%); Crotonic acid (2.64%); 2-Propanone, 1,1-diethoxy (0.18%); 2-Butanone, 4-hydroxy (13.11%); 1-Tetrazol-2-ylethanone (86.4%); Propanethioamide (0.49%); Acetic acid, ethyl ester; Acetidin (100%); Methylolacetone (100%) and Trichoderma hamatum (T-113) were identified: Ethane, 1,1-diethoxy (100%) and 2-Butanone, 4-hydroxy-(100%).

  1. Alikhani, H.A., Saleh-Rastin, N. and Autoun, H. (2006). Phosphate solubilization activity of rhizobia native to Iranian soils. Plant and Soil. 287: 35-41.

  2. Al-Taie, A.H. (2014). Effect of Aspergillus spp and Trichoderma hamatum on growth of cucumber Cucumis sativus planting in compost soilless culture. Ph.D Dessitation, College of Agriculture. University of Kufa, Najaf, Iraq.

  3. Al-Taie, A.H., Matrood, A.A. and Al-Asadyi, M. (2016). The influence of some fungi bio-genic on promoting growth and yield of wheat-Var. Ibaa99. International Journal Current Microbiology Apply Science. 5(11): 757-764. 

  4. Al-Taie, A.H. and Alwan, S.L. (2014).Effectiveness of some isolate of Aspergillus and Trichoderma hamatum that isolated from compost plant waste in solubilization of phosphate in solid and broth media. Kufa Journal for Agricultural Sciences. 6(4): 59-81.

  5. Barroso, C.B. and Nahas, E. (2008). Solubilization of iron phosphate in culture medium. Brazilian Agricultural Research. 43(4): 529-535.

  6. Bibi, Y., Nisa, S., Chaudhary, F.M. and Zia, M. (2011). Antibacterial activity of some selected medicinal plants of Pakistan. Biomedical Central Complementary and Alternative Medicine. 11(52): 1-7.

  7. Calvo, A.M., Wilson, R.A., Bok, J.W. and Keller, N.P. (2002). Relationship between secondary metabolism and fungal development. Microbiology and Molecular Biology Reviews. 66: 447-59. 

  8. Edi-Premono, M., Moawad, A.M. and Vleck, P.L. (1996). Effect of phosphate solubilizing Pseudomonas putida on the growth of maize and its survival in the Rhizosphere. Indonesian Journal Crop Science. 11: 13-23. 

  9. El-Azouni, I.M. (2008). Effect of phosphate solubilizing fungi on growth and nutrient uptake of soybean (Glycine max L.) plants. Journal Apply Science Research. 4(6): 592- 598.

  10. Elkot, G.A. and Derbalah, A.S. (2011). Use of culture filtrates of certain microbial isolates for powdery mildew control in squash. Journal of Plant Protection Research. 51(3).

  11. Hamdali, H., Hafidi, M., Virolle, M.J. and Ouhdouch, Y. (2008). Rock phosphate solubilizing actinomycetes: Screening for plant growth-promoting activities. World Journal of Microbiology and Biotechnology. 24: 2565-2575.

  12. Javaid, A. (2009). Arbuscular mycorrhizal mediated nutrition in plants. Journal of Plant Nutrition. 32(10): 1595-1618.  

  13. Khalaf, M.A., El-Zaawely, A.A., Al-Taie, A.H. and Elsheery, N.I. (2018). Antifungal activity of some plant extracts and Trichoderma spp. against cucumber damping off caused by Pythium aphanidermatum.. Journal of Biodiversity and Environmental Sciences. 12(6): 195-203.

  14. Khan, I.H. and Javaid, A. (2021). In vitro screening of Aspergillus spp. for their biocontrol potential against Macrophomina phaseolina. Journal of Plant Pathology. 103(4): 1195- 1205.                                                                                                                        

  15. Li, A. and Punt, P. (2013). Industrial Production of Organic Acids by Fungi. In: Applications of Microbial Engineering. [Gupta V., Schmill M., Mazutti M., Mäki M., Tuohy M. (Eds.)], CRC Press, Boca Raton, pp. 52-74.

  16. Magnuson, J.K. and Lasure, L.L. (2004). Organic Acid Production by Filamentous Fungi. In: Advances in Fungal Biotechnology for Industry, Agriculture and Medicine. Edited by Tkacz, J.S. and Lange, L.: Springer. pp; 307–340.

  17. Mehta, S. and Nautiyal, C.S. (2001). An efficient method for qualitative screening of phosphate solubilizing bacteria. Current Microbiology. 43: 51-56.

  18. Nemec, T., Jernejc, K. and Cimerman, A. (1997). Sterols and fatty acids of different Aspergillus species. FEMS Microbiology Letters. 149: 201-2015. 

  19. Nenwani, V., Doshi, P., Saha, T. and Rajkumar, S. (2010).Isolation and characterization of a fungal isolate for phosphate solubilization and plant growth promoting activity. Journal of Yeast and Fungal Research. 1(1): 009-014

  20. Parekh, J., Jadeja, D. and Chanda, S. (2005). Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turkish Journal of Biology 29: 203-210.

  21. Pikovaskya, R.I. (1948). Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya. 17: 362-370.

  22. Raaijmakers, J.M., Paulitz, T.C., Steinberg, C., Alabouvette, C. and Moenne-Loccoz, Y. (2009). The Rhizosphere: A playground and battlefield for soilborne pathogens and beneficial microorganisms. Plant and Soil. 321: 341-61.

  23. Saber, W.I., Ghanem, K.M. and El-Hersh, M.S. (2009). Rock phosphate solubilization by two isolates of Aspergillus niger and Penicillium sp. and their promotion to mung bean plants. Research Journal of Micrabiology. 4(7): 235-250.

  24. Schalchli, H., Hormazabal, E., Becerra, J., Birkett, M., Alvear, M., Vidal, J. and Quiroz, A. (2011). Antifungal activity of volatile metabolites emitted by mycelial cultures of saprophytic fungi. Chemistry and Ecology. 27: 503-513.

  25. Whitelaw, M.A. (2000). Growth promotion of plants inoculated with phosphate solubilizing fungi. Advances in Agronomy. 69: 99-151.

  26. Whitelaw, M.A., Harden, T.J. and Helyar, K.R. (1999). Phosphate solubilisation in solution culture by the soil fungus Penicillium radicum. Soil Biology Biochemistry. 31: 655-665.

  27. Xiao, C.Q., Zhang, H.X., Fang, Y.J. and Chi, R.A. (2013). Evaluation for rock phosphate solubilization in fermentation and soil- plant system using a stress-tolerant phosphate-solubilizing Aspergillus niger WHAK1. Applied Biochemistry and Biotechnology. 169(1): 123-33. 

  28. Xiao, C.Q., Fang, Y.J. and Chi, R.A. (2015). Phosphate solubilization in vitro by isolated Aspergillus niger and Aspergillus carbonarius. Research on chemical Intermediates. 41(5): 2867-2878.

  29. Yuan, Q.P., Wang, J.D., Zhang, H. and Qian, Z.M. (2005). Effect of temperature shift on production of xylanase by Aspergillus niger. Process Biochemistry. 40: 3255-3257.

  30. Zayed, G. and Motaal, H.A. (2005).Bioactive compost from rice straw enriched with rock phosphate and their effect on the phosphorus nutrition and microbial community in Rhizosphere of cowpea. Bioresource Technology. 96: 929-935.

  31. Zeng, R.S., Luo, S.M., Shi, M.B., Shi, Y.H., Zeng, Q. and Tan, H.F. (2001). Allelopathy of Aspergillus japonicus on crops. Agronomy Journal. 93: 60-64.

Editorial Board

View all (0)