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​The Histopathology of Alimentary Canal of Stinging Catfish Heteropneustes fossilis (Bloch, 1794), Susceptible for Trematodes Incursion

DOI: 10.18805/ag.D-5366    | Article Id: D-5366 | Page : 233-237
Citation :- ​The Histopathology of Alimentary Canal of Stinging Catfish Heteropneustes fossilis (Bloch, 1794), Susceptible for Trematodes Incursion.Agricultural Science Digest.2022.(42):233-237
Pooja Vishwakarma, Ramakant Maurya, Anand M. Saxena poojavishwakarma19900@gmail.com
Address : Department of Zoology, Helminthology Laboratory, University of Lucknow, Lucknow-226 007, Uttar Pradesh, India.
Submitted Date : 1-05-2021
Accepted Date : 15-06-2021

Abstract

Background: Heteropneustes fossilis (Bloch, 1794) is one of the popular freshwater fish known for its food quality and nutritional value. This study was undertaken with an aim to find out histopathological changes in the intestine of freshwater fish Heteropneustes fossilis, host of trematode parasite Masenia vittatusia Agarwal, 1963.
Methods: The intestine of uninfected fish used to determine normal anatomy, while infected intestine to determine pathological changes. The normal histological procedure was followed, which included fixing, rinsing, dehydrating and embedding of tissue. Tissue was cut in 4-6 µm thick sections using a rotary microtome. Haematoxylin and eosin stains were used.
Result: The highest damage was observed in the mucosal layer with ruptured and fused microvilli, hyperplasia of villi, damaged columnar epithelium layer and spread of loose connective tissue into the lumen. Other degenerative modifications included hypertrophy of blood vessels in mucosa and muscularis part, some of them ruptured might be the cause of haemorrhage inside the layers. Other changes include variation in the routine shape of all three layers. The mucosal layer showed aggregation of lymphocytes and mast cells as well.

Keywords

​Heteropneustes fossilis Hypertrophy Hyperplasia lymphocytes Masenia vittatusia Trematode


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