​Regeneration of Stem Cuttings of Pomegranate cv. Bhagwa as Influenced by PGR’s and Planting Time

DOI: 10.18805/ag.D-5280    | Article Id: D-5280 | Page : 32-37
Citation :- ​Regeneration of Stem Cuttings of Pomegranate cv. Bhagwa as Influenced by PGR’s and Planting Time.Agricultural Science Digest.2022.(42):32-37
Gurpreet Kaur, Sukhdev Singh gurpreetkdeol9@gmail.com
Address : Department of Horticulture (Agriculture), Khalsa College, Guru Nanak Dev University, Amritsar-143 001, Punjab, India.
Submitted Date : 30-11-2020
Accepted Date : 29-05-2021


Background: Pomegranate is being cultivated in the tropical and sub-tropical parts of the world for its delicious fruits. It can be propagated from seeds as well as from softwood, semi-hardwood and hardwood cuttings. The application of auxins encourages rooting in stem cutting owing to their ability to active cambium regeneration, cell division and cell multiplication. To meet the demand of pomegranate in the current situation and owing to its importance, the present study was undertaken.
Methods: For regeneration of stem cuttings of pomegranate cv. Bhagwa as influenced by PGR‘s and planting time a study was conducted at the Department of Horticulture, Khalsa College, Amritsar during 2019-2020. The trial was undertaken with ten treatments comprising of IBA, PHB and NAA (500, 750 and 1000 ppm) each by quick dip method along with control planted in the first week of August and January. 
Result: The results of the study revealed that IBA 1000 ppm (T3) proved to be the best in terms of minimum days to first sprouting (11.06), maximum sprouting (91.10%), rooting (83.31%), number of roots per cutting (60.03), root length (11.55 cm), fresh weight of roots (1.08 g) and dry weight of roots (0.55 g). Among planting time the cuttings planted on first week of January  proved to be effective in terms of sprouting (87.83%), survival (84.70%), rooting (78.81%) and number of roots (52.06) respectively.


​Cuttings Planting time Pomegranate Quick dip Rooting Sprouting Survival


  1. Adelson, F.D. (2009). Rooted stem cutting of the olive tree in different times, substrates and doses of IBA. Cienc Agrotec. 33: 79-85.
  2. Chalfun, N.N.J., Pasqual, M., Norberto, P.M., Dutra, L.F. and Alves, J.M.C. (2003). Rooting of fig (Ficus carica L.) cuttings: Cutting time and IBA. Acta Hort. 605.
  3. Chandramouli, H. (2001) Influence of growth regulators on the rooting of different types of cuttings in Bursera penicillata (DC). M.Sc Thesis University of Agri Sci Banglore.
  4. Correa, L.D.R., Paim, D.C., Schwambach, J. and Fett-Neto, A.G. (2005). Carbohydrates as regulatory factors on the rooting of Eucalyptus saligna Smith and Eucalyptus globules Labil. Plant Growth Reg. 45: 63-73.
  5. Deb, P., Bhowmick, N., Ghosh, S.K. and Suresh, C.P. (2009). Effect of different concentrations of NAA and IBA on success and growth of semi hardwood cutting of lemon (Citrus limon). Environ and Eco. 27: 1130-31.
  6. Diwaker and Katiyar, P.N. (2013). Regeneration of kagzi lime (Citrus aurantifolia Swingle) through stem cuttings with the aid of IBA and PHB. Hort flora Res Spe. 2: 271-73.
  7. Galavi, M., Karimian, M.A. and Mousavi, S.R. (2013). Effects of different auxin (IBA) concentrations and planting-beds on rooting grape cuttings. Annual Review and Res Bio. 3: 517-23.
  8. Hartmann, H.T., Kester, D.E., Devies, F.T. and Geneve, R.L. (2007). Plant Propagation principles and practices. Seventh Edition, Prentice Hall of India Pvt. Ltd., New Delhi.
  9. Hartmann, H.T., Kester, D.E., Davis, F.T. and Genev, R.L. (2002). Plant Propagation: principles and practices. Prentice Hall, Englewood Cliffs 880.
  10. Husen, A. (2008). Clonal propagation of Dalbergia sissoo Roxb. and associated metabolic changes during adventitious root primordium development. News Forest. 36: 13-27.
  11. Iqbal, M., Subhan, F., Ghafoor, A. and Jilani, M.S. (1999). Effect of different concentrations of IBA on root initiation and plant survival of apple cuttings. Pakistan J. Bio. Sci. 2: 1314- 16.
  12. Kahramanoglu, I. and Umar, K. (2018). Effects of cutting length, time and growing media on sprouting of dormant semi- hardwood cuttings of pomegranate cv. Wonderful. African J. Agri Res. 13: 2587-90.
  13. Karimi, H.R. and Mirdehghan, S.H. (2013). Correlation between the morphological characters of pomegranate (Punica granatum L.) traits and their implications for breeding. Turkish J. Bot. 37: 355-62.
  14. Kaur, A., Kaur, K. and Kaur, A. (2018). Role of IBA and PHB on success of cuttings of Fig cv. Brown Turkey. Asian J. Sci. Tech. 9: 8237-41.
  15. Kaur, S. and Kaur, A. (2016). Effect of IBA and PHB on rooting of pomegranate (Punica granatum L.) cuttings cv. Ganesh. Bio Forum. 8: 203-06.
  16. Kishorbhai, B.S. (2014). Effect of plant growth regulators on propagation of fig (Ficus carica L.) by hardwood and semi hardwood cuttings. M.Sc. Thesis Navsari Agricultural University.
  17. Krieken, W.M., Breteler, H., Visser, M.H.M and Mavridon, D. (1990). The role of the conversion of IBA into IAA on root regeneration in apple: Introduction of test system. Plant cell report. 12: 203-06.
  18. Kumar, R., Gill, D.S. and Kaushik, R.A. (1995) Effect of Indole butyric acid, p-hydroxybenzoic acid and season on the propagation of lemon cv. Baramasi form cuttings. Haryana J. Hort. Sci. 24: 13-8.
  19. Kumar, R., Gora, J.S., Kumar, R., Singh, A., Kumar, A. and Gajender (2016). Establishment, survival and growth of pomegranate cuttings with different concentrations of Indolebutyric acid and rooting substrates. Eco Env and Cons. 22: 321-27.
  20. Kumar, S., Shukla, H.S. and Kumar, S. (2004). Effect of IBA (Indole butryric Acid) and PHB (p-hydroxy benzoic acid) on the regeneration of sweet lime (Citrus limettioides Tanaka) through stem cuttings. Prog Agri. 4: 54-6.
  21. Mehta, S.K., Singh, K.K. and Harsana, A.S. (2018). Effect of IBA concentration and time of planting on rooting in pomegranate (Punica granatum) cuttings. J. Med. Plant Studies. 6: 250-53.
  22. Narula, S. and Kaur, G. (2018). Influence of growth regulators on the regeneration and survival of plum cv. Kala Amritsari through stem cuttings. Inter J. Agri Innov Res. 7: 320-24.
  23. Narula, S. (2018). Effect of growth regulators on rooting of cuttings in plum cv Kala Amritsari. Scholarly Res J Humanity Sci and Eng Language. 5: 6889-96.
  24. Panwar, R.D., Gupta, A.K., Saini, R.S. and Sharma, J.R. (2001). Effect of auxin on the rooting of cuttings in Bougainvillea var. Mary Palmer. Haryana J. Hort Sci. 30: 215- 16. 
  25. Patil, V.N., Chauhan, P.S., Panchbhai, D.M., Shivankar, R.S. and Tannirwar, A.V. (2000). Effect of different growth regulators on rooting of hardwood cuttings of some commercial grape varieties. J. Soils Crops. 10: 295-97. 
  26. Pirlak, L. (2000). Effect of different cutting times and IBA doses on the rooting rate of hard wood cuttings of cornelian cherry (Cornus mas L.). Atatark Univ Dept of Hort. 10: 122-34.
  27. Polat, A.A. and Caliskan, O. (2009). Effect of Indole butyric acid (IBA) on rooting of cuttings in various pomegranate genotypes. Acta hort (ISHS). 818: 187-92.
  28. Rafael, D. (2005). Rooting of different types of olive (Olea europaea L.) tree cuttings using indole butyric acid. Cienc Agrotec. 29: 562-67.
  29. Ram, R.B., Kumar, P. and Kumar, A. (2005). Effect of IBA and PHB on regeneration of pomegranate (Punica granatum L.) through stem cuttings. New Agriculturist. 16: 113-15.
  30. Reddy, K.V.R., Reddy, C.P. and Goud, P.V. (2008). Effect of auxins on the rooting of fig (Ficus carica L.) hardwood and semi hardwood cuttings. Indian J. Agri Res. 42: 75 -78.
  31. Rymbai, H. and Reddy, G.S. (2010) Effect of IBA, time of layering and rooting media on air-layers and plantlets survival under different growing nursery conditions in guava. Indian J Hort. 67: 99-104. 
  32. Seiar, Y.A. (2017). Effect of growth regulators on rooting of cuttings in pomegranate (Punica granatum L.) cv. Bhagwa. J. Hort Sci. 11: 156-60.
  33. Sharma, N., Anand, R. and Kumar, D. (2009). Standardization of pomegranate (Punica granatum L.) propagation through cuttings. Biological Forum. 1: 75-80.
  34. Sharma, S. (1999). Effect of type of cuttings IBA and time of planting on rooting of cuttings in pomegranate (Punica granatum L.) cv. Ganesh. M.Sc. Thesis GNDU Amritsar.
  35. Shukla, H.S., Tripathi, V.K., Awasthi, R.D. and Tripathi, A.K. (2010). Effect of IBA, PHB and boron on rooting and shoot growth of hardwood stem cuttings of peach. Inter J App Agri Res. 5: 467-73.
  36. Singh, B., Singh, S. and Singh, G. (2011). Influence of planting time and IBA in rooting and growth of pomegranate (Punica granatum L.) cv. Ganesh cuttings. Acta Hort (ISHS). 890: 183-88. 
  37. Singh, D.B., Sharma, B.D. and Bhargava, R. (2003). Effect of boron and GA3 to control fruit cracking in pomegranate (Punica granatum). Current Agri. 27: 125-27.
  38. Singh, K.K. (2017). Vegetative propagation of pomegranate (Punica granatum L.) through cutting A review. Inter J. Curr Microbiol App Sci. 6: 4887-93.
  39. Singh, K.K. (2013). Effect of IBA concentrations on the rooting of pomegranate (Punica granatum L.) cv. Ganesh hardwood cuttings under mist house condition. Plant Archives. 14: 1111-14.
  40. Thota, S., Madhavi, K. and Vani, V.S. (2012). Effect of type of cuttings and IBA concentrations on the propagation of fig. Inter J Tropical Agri. 32: 89-94.
  41. Tripathi, S.N. and Shukla, H.S. (2004). Propagation of pomegranate (Punica granatum L.) cvs. by stem cuttings with indole butyric acid and p-hydroxy benzoic. Indian J. Hort. 61: 362-65.
  42. Upadhyay, S.D. and Badyal, J. (2007). Effect of growth regulators on rooting of pomegranate (Punica granatum L.) cutting. Haryana J Hort Sci. 36: 58-9.
  43. Yagoub, H.A.R.I. (1998). Rooting of pomegranate (Punica granatum L.) cuttings in response to position, season and application in Indole butyric acid (IBA) rooting hormone. M.Sc. Thesis Univ of Sinnar, Sudan.

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