Agricultural Science Digest

Screening of Milk Borne Staphylococcus aureus for Resistance against Beta Lactam Antibiotics

DOI: 10.18805/ag.D-5123    | Article Id: D-5123 | Page : 113-115
Citation :- Screening of Milk Borne Staphylococcus aureus for Resistance against Beta Lactam Antibiotics.Agricultural Science Digest.2021.(41):113-115
B. Anurag, T. Ramasamy, S. Ramesh, K.S. Sriraam, L. Kalaiselvi, S.J. Deepak, R. Gokula Kannan, C.S. Arunaman drrams19@gmail.com
Address : Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, Tamil Nadu, India.
Submitted Date : 2-01-2020
Accepted Date : 9-07-2020
First Online:

Abstract

Background: A study was carried out to screen milk borne Staphylococcus aureus for resistance against Beta lactam antibiotics. 
Methods: A total of 45 milk samples were collected over a period of three months from large animal outpatient unit of Madras Veterinary College Hospital, Chennai. Upon collection of samples, ABST followed by its growth in Mannitol Salt Agar was carried out as part of the phenotypic screening. Genotypic screening for Staphylococcus screening was done with the help of PCR by using nuc  and mec A primers. MIC for ceftriaxone and cloxacillin was carried out with the samples that were found positive for Staphylococcus aureus. The antibiotic sensitivity pattern is presented: Fluoroquinolones (87.5% sensitive), aminoglycosides (72.5% sensitive), Amoxicillin-Clavulanic acid (Amoxyclave) (72.5% sensitive). The MSA positive samples were subjected to molecular identification with the help of PCR. 
Result: The results revealed 10 samples positive for Staphylococcus aureus and 5 among them positive for mecA gene. The MIC results were as follows: MIC50-10.95µg/ml and MIC90- 87.510.95µg/ml for ceftriaxone and MIC50- 43.75 µg/ml and MIC90- 87.5µg/ml for cloxacillin, indicating emergence of resistance. However, further studies are required in a larger sample size that can help us to attain more conclusive results.

Keywords

ABST Ceftriaxone Cloxacillin MIC PCR

References

  1. Andrews, J.M. (2001). Determination of minimum inhibitory concentrations. The Journal of antimicrobial chemotherapy. 48: 5-16.
  2. Awandkar, S.P., Bhikane, A.U. and Kulkarni, M.B. (2013). Antibiotic resistance trends in clinical bovine mastitis. Biolife. 1: 139-143
  3. Balouiri, M., Sadiki, M. and Ibnsouda, S.K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical Analysis. 6: 71-79.
  4. Blair, E.B., Emerson, J.S. and Tull, A.H. (1967). A new medium, salt mannitol plasma agar, for the isolation of Staphylococcus aureus. American. Journal of Clinical Pathology. 47: 30-39.
  5. Brakstad, Odd, G., Kjetill, Aasbakk. and Johan, A., Maeland. (1992) Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. Journal of. Clinical. Microbiology. 30: 1654-1660.
  6. Chapman, G.H. (1945). The significance of sodium chloride in studies of Staphylococci. JBacterio. 50: 201-203.
  7. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing: 20th informational supplement. Wayne, PA: CLSI; 2010.
  8. Kumar, N., Manimaran, A., Sivaram, M., Kumaresan, A., Jayekumar, S., Sreela, S., Moovethan, P. and Rajendran, D. (2017). Influence of clinical mastitis and its treatment outcome on reproductive performance in crossbred cows: A retrospective study. Veterinary World. 10: 485-492.
  9. Pournajaf, Abazar., Abdollah, Ardebili., Leyla, Goudarzi., Mahmoud, Khodabandeh., Tahmineh, Narimani. and Hassan Abbaszadeh. (2014). PCR-based identification of methicillin–resistant Staphylococcus aureus strains and their antibiotic resistance profiles. Asian Pacific journal of Tropical Biomedicine. 4: S293-S297.
  10. Rainard, P., Foucras, G., Fitzgerald, J.R., Watts, J.L. and Middleton J.R. (2017) Knowledge gaps and research priorities in Staphylococcus aureus mastitis control. Transboundary and Emerging Diseases. 65 : 149-165.
  11. Shields, P. and Tsang, A.Y. (2006). Mannitol salt agar plates protocols. Available from the MicrobeLibrary website: http://www. microbelibrary. org/library/laboratory-test/3034-mannitol-salt-agar-plates-protocols.
  12. Verma, H., Rawat, S., Sharma, N., Jaiswal, V. and Singh R. (2018). Prevalence, bacterial etiology antibiotic susceptibility pattern of bovine mastitis in meerut. Journal of Entomology and Zoological Studies. 6: 706-709.
  13. Wiegand, I., Hilpert, K. and Hancock, R.E. (2008). Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nature protocols. 3:163.
  14. Zeryehun, T. and Abera, G. (2017) Prevalence and bacteriological isolates of mastitis in Dairy farms in selected districts of Eastern Harrarghe Zone, Eastern Ethiopia. Journal of Veterinary Medicine. Article ID 6498618. 

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