Agricultural Science Digest

  • Chief EditorArvind kumar

  • Print ISSN 0253-150X

  • Online ISSN 0976-0547

  • NAAS Rating 5.52

  • SJR 0.156

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Agricultural Science Digest, volume 37 issue 2 (june 2017) : 106-111

In-vitro anti-bacterial activity and phytochemical screening of crude extracts of Catharanthus roseus L. (G.) Don.

Jyoti Rani, Manish Kapoor*, Rupinder Kaur
1<p>Department of Botany, Punjabi University, Patiala-147 001, Punjab, India.</p>
Cite article:- Rani Jyoti, Kapoor* Manish, Kaur Rupinder (2017). In-vitro anti-bacterial activity and phytochemical screening of crude extracts of Catharanthus roseus L. (G.) Don. . Agricultural Science Digest. 37(2): 106-111. doi: 10.18805/asd.v37i2.7983.

ABSTRACT

Present study aims to investigate phytochemical analysis as well as in vitro antimicrobial activity of crude aqueous, methanol, ethanol, chloroform and petroleum ether extracts from leaves of C. roseus. Antimicrobial activity of extract was studied against various bacterial strains (Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Staphylococcus aureus and Mycobacterium smegmatis).  Qualitative preliminary phytochemical analysis revealed that alkaloids, phenols, flavonoids, amino acid and cardiac glycosides are present in the extracts. The result for total phenol and flavonoids content was the highest in methanol and the lowest in petroleum ether crude extract.  The study revealed that inhibition significantly depend upon the solvent used for extraction and bacterial strain tested for susceptibility. Aqueous extracts were found less efficient as compared to organic solvent. Methanolic extract were found more effective against tested microbes. 

REFERENCES


  1. Aligiannis N, Kalpoutzakis E, Mitaku S and Chinou IB (2001). Composition and antimicrobial activity of the essential oils two Origanum species. J. Agric. Food. Chem., 49: 4168-4170.

  2. Ayoola GA, Coker HA, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC and Atangbayila TO (2008). Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop. J. Pharmaceul. Res.,7: 1019-1024.

  3. Balaabirami S and Patharajan S (2012). In-vitro antimicrobial and antifungal activity of Catharanthus roseus leaves extract against important pathogenic organisms. Int. J. Pharm. Pharm. Sci., 4: 487-490.

  4. Dubey M and Sushma (2014). Phytochemical status of some selected medicinal plants (Eclipta alba, Catharanthus roseus and Swertia chirata). Asian. J. Plant. Sci. and Res., 4: 28-34.

  5. Gobalakrishnan R, Kulandaivalu M, Bhuvaneswari R, Kandavel D and Kannan, L (2013). Screening of wild plant species for antibacterial activity and phytochemical analysis of Tragia involucrate L. J. Pharm. Anal., 07: 01- 06.

  6. Gomez, K.A., and Gomez, A.A. (1984). Statistical Procedures for Agricultural Research. 2nd ed., John Wiley and Sons, New York.

  7. Govindasamy C and Srinivasan R (2012). In-vitro antibacterial activity and phytochemical analysis of Catharanthus roseus (Linn.) G. Don. Asian. Pac. J. Trop. Biomed., 2: 155-158.

  8. Goyal P, Khanna A, Chauhan A, Chauhan G and Kaushik P (2008). In-vitro evaluation of crude extracts of Catharanthus roseus for potential antibacterial activity. Int. J. Green. Pharm., 2: 176.

  9. Hema TA, Shiny M and Parvathy J (2012). Antimicrobial activity of leaves of Azima tetracantha against clinical pathogens. Int. J. Pharm. Sci., 4: 317-319.

  10. Hossain S, Parvin S, Dutta S, Islam K, Saha MR and Islam E (2014). Evaluation of antimicrobial activities of methanolic extract and fractions of Ficus hispida (Linn.) fruits. Int. J. Phytopharmacology., 5: 390-393.

  11. Igbinosa OO, Igbinosa QC and Aiyegoro OA (2009). Antimicrobial activity and phytochemical screening of stem bark extracts from Jatropha curcas (Linn). Afr. J. Pharm. Pharmacol., 3: 58-62.

  12. Islam MR, Ahamed R, Rahman MO, Akbar MA, Al-Amin M, Alam KD and Lyzu F (2010). In vitro antimicrobial activities of four medicinally important plants in Bangladesh. Eur. J. Sci. Res., 39: 199-206.

  13. Khandewal KR (2008). Practical Pharmacognocy. Nirali Prakashan, Pune, 19th ed edition.

  14. Kokate CK, Purohit AP and Gokhale SB (2001). Carbohydrate and derived Products, drugs containing glycosides, drugs containing tannins, lipids and protein alkaloids. Text book of Pharmacognosy, 7th ed. India: Nitrali Prakashan. 

  15. Kumar M, Nehra K and Duhan JS (2013). Phytochemical analysis and antimicrobial efficacy of leaf extracts of Pithecellobium dulce. Asian. J. Pharm. Clin. Res., 6: 70-76.

  16. Kumar S, Chaudhary S, Kumari R, Sharma V and Kumar A (2012). Development of improved horticultural genotypes characterized by novel over-flowering inflorescence trait in periwinkle Catharanthus roseus. Proceedings of the National Academy of Sciences India Section B: Biological Sciences., 82: 399–404.

  17. Kumar S, Malayaman V and Sindhuja S (2013). Phytochemical screening and antibacterial evaluation of the leaf, flower and seed coat extracts of Cassia alata L. J. Chem. Pharm. Res., 5:740-744.

  18. Mathew BB, Jatawa SK and Tiwari A (2012). Phytochemical analysis of Citrus limonum pulp and peel. Int. J. Pharm. PharmSci., 4: 145-148.

  19. Mir MA, Sawhney SS and Jassal MMS (2013). Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale. Wudpecker. J. Pham. Phamacol., 2: 1-5.

  20. Omwenga EO, Okemo PO, Mbugua PK and Ogol C (2009). Ethnobotanical survey and antimicrobial evaluation of medicinal plants used by the Samburu community (Kenya) for treatment of diarrhoea. Pharmacogn. Mag., 5: 165.

  21. Park YS, Jung ST, Kang SG, Heo BG, Arancibia-Avila P, Toledo F, Drzewiecki J, Namiesnik J and Gorinstein S (2008). Antioxidants and proteins in ethylene- treated kiwifruits. Food. Chem., 107: 640-648.

  22. Rashid T S, Sijam K, Kadir J, Saud H M, Awla H K, Zulperi D and Hata E M (2016). Screening for active compounds in Rhus coriaria L. crude extract that inhibit the growth of Pseudomonas syringae and Ralstonia solanacearum. Indian J. Agric. Res., 50: 15-21.

  23. Rischer H, Oresic M, Seppanen-Laakso T, Katajamaa M, Lammertyn F, Ardiles-Diaz W, Van Montagu MC, Inze D, Oksman-Caldentey KM and Goossens A (2006). Gene to metabolite network for terpenoid indole alkaloid biosynthesis in Catharanthus roseus cells. Proc. Natl. Acad. Sci U.S .A., 103:5614-9.

  24. Sathiya S, Karthikeyan B, Jaleel CA, Azooz MM and Iqbal M (2008). Antibiogram of Catharanthus roseus extracts. Global. J. Mol Sci., 3: 01-07.

  25. Singh S, Kaur R and Sharma SK (2013). Pharmacognostical standardization of the roots of Rumex hastatus D. Don. Asian. J. Pharm. Clin. Res., 6: 126-128.

  26. Singleton VL and Rossi JA (1965). Colourimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J Enol. Vitic., 16: 144-158. 

  27. Susmit K and Archana T (2011). Viewing anti-diabetic assets and analysis of stevioside from Stevia rebaudiana crude extract in STZ-    induced diabetic BALB/CAN. N (IB) mice. J. Pharm. Res., 4: 112-117.

  28. Thakur R, Singh R and Jain N (2012). Evaluation of antibacterial activity of Sphaeranthus indicus L. leaves. J. Pharm. Res., 5: 4382-4388.

  29. Ugochukwu SK, Uche A and Ifeanyi O (2013). Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala G. Baker. Asian. J Plant. Sci and Res., 3:10-13.

  30. Vijayalakshmi K, Immanuel S, Sindhu C and Arumugam P (2014). In-Vitro Investigation of Antidiabetic Potential of Selected Traditional Medicinal Plants. Int. J. Pharmacognosy and Phytochem. Res., 2: 856-861.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)