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Research Article

Agricultural Science Digest, volume 36 issue 4 (december 2016) : 295-298

Biochemical study of root extract of Withania somnifera (L.) plant through HPLC analysis 

Naveen Gaurav1, Arun Kumar1, Aditi Grover1, Deepak Som2, U.K. Chauhan3, A. P. Singh4
1<p>Department of Biotechnology,&nbsp;S G R R P G College, Dehradun- 248 001, U.K.</p>
Cite article:- Gaurav1 Naveen, Kumar1 Arun, Grover1 Aditi, Som2 Deepak, Chauhan3 U.K., Singh4 P. A. (2016). Biochemical study of root extract of Withania somnifera (L.)plant through HPLC analysis . Agricultural Science Digest. 36(4): 295-298. doi: 10.18805/asd.v36i4.6471.

ABSTRACT

Secondary metabolite contents of W. somnifera varied remarkably between seasons and genotypes under ex vitro condition. In vitro studies provide an optimum culture condition for steady and quality production of bioactive chemicals throughout the year without involvement of environmental stresses. Mass production of micro-shoots and plantlets by exploring the organogenic totipotency of shoot tip explants (ex vitro and in vitro grown) considering two elite genotypes (Poshita and Jawahar 22) of W. somnifera, and assessment of their capability in production and accumulation of bioactive metabolites (total alkaloid and withanolides amount were quantified; withanolide A and withaferin A contents were estimated by High Performance Liquid Chromatography-HPLC).

REFERENCES


  1. Amirkhani M., Lambardi M., Mesdaghi M., Mashayekhi K., Ozudogru E.A. (2010). In vitro morphogenesis and plant regeneration from seed- and leaf-derived cali of four Agropyron species. Propagation of Ornamental Plants, 10: 99-106.

  2. Anonymous. (2004). Monograph: Withania somnifera. Altern. Med. Rev. 9: 211-214.

  3. Atta-ur-Rahman., Abbas, S., Jamal, A.S. and Choudhary, M.I. (1993). New withanolides from Withania spp. J. Nat. Prod., 56: 1000-1006.

  4. Atta-ur-Rahman., Jamal, A.S., Choudhary, M.I. and Asif, I. (1991). Two withanolides from Withania somnifera. Phytochem., 30: 3824-3825.

  5. Bandyopadhyay, M., Jha, S., and David T. (2007). Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera. Plant Cell Rep., 26:599–609.

  6. Chaurasiya, N.D., Uniyal, G.C., Lal, P., Misra, L., Sangwan, N.S., Tuli, R. and Sangwan, R.S. (2008). Analysis of withanolides in root and leaf of Withania somnifera by HPLC with photodiode array and evaporative light scattering detection. Phytochem Anal, 19:148–154.

  7. Choudary, M.I., Abbas, S., Jamal, A.S. and Atta-ur-Rahman. (1996). Withania somnifera- A source of exotic withanolides. Heterocycles, 42: 555-563.

  8. Dalavayi, S., Kulkarni, S.M., Rajyalakshmi, I. and Itikala, S. (2006). Determination of Withaferin-A in two Withania Species by RP-HPLC method, Indian Journal of Pharmaceutical Sciences, 68: 253-256.

  9. Kapoor, L.D. (2001). Handbook of Ayurvedic Medicinal Plants; CRC Press: London, UK, 2001; pp. 337-338.

  10. Khajuria, R.K., Suri, K.A., Gupta, R.K., Satti, N.K., Amina, M., Suri, O.P. and Qazi, G.N. (2004). Separation, identification and quantification of selected withanoides in plant extracts of Withania somnifera by HPLC-UV (DAD) – positive ion electrospray ionization – mass spectrometry. J Sep Sci., 27:541–546.

  11. Khan, N., Ragini, G., Shrivastava, N.M. and Shrivastava, S. (2014). Evaluate the Effect of Withania Somnifera Methanolic Extracts as In-Vitro Anti-Glycating Agents, Octa Journal of Biosciences ISSN 2321 – 3663; International peer-    reviewed journal June 2014 Octa. J. Biosci., 2:13-17.

  12. Kirson, I., Glotter, E., Lavie, D. and Abraham, A. (1971), Constituents of Withania somnifera Dun. XII. The withanolides of an Indian chemotype. J. Chem. Soc., 1971,C, 2032-2044.

  13. Lal, P., Misra, L., Sangwan, R.S. and Tuli, R. (2006). New withanolides from fresh berries of Withania somnifera. Z Naturforsch, 61b: 1143–1147.

  14. Mirjalili, M.H., Moyano, E., Bonfill, M., Cusido, R.M. and Palazón J. (2009). Steroidal lactones from Withania somnifera, an ancient plant for novel medicine. Molecules, 2009; 14 (7): 2373–93.

  15. Murashige, T. and Skoog, F. (1962). “A revised medium for rapid growth and bioassay with tobacco tissue culture”, Physiol plant, 48: 473-497.

  16. Rana, S., Dhar, N., Wajid, W.B., Razdan, S., Khan, S., Rekha, S.D., Dutt, P. and Lattoo, S.K. (2012). A 12-    deoxywithastramonolide-rich somaclonal variant in Withania somnifera (L.) Dunal—molecular cytogenetic analysis and significance as a chemotypic resource The Society for In Vitro Biology.

  17. Rastogi, R.P. and Mehrotra, B.N. (1998). Compendium of Indian Medicinal Plants. 2nd Reprint, Central Drug Research Institute, Lucknow and National Institute of Science Communication, Council of Scientific and Industrial Research, New Delhi (1998). 1: 434-436; Vol. 2: 708-710; Vol. 3: 682-684; Vol. 4: 765-766; Vol. 5: 889-891; Vol. 6: 148.

  18. Shashi, B., Govindarajan, R., Rawat, A.K. and Mehrotra, A. (2004). HPLC analysis of Withaferin A in Withania somnifera (L.) dunal. J. Pharmacological Science, 66: 236-238.
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